以梧州霉豆渣为对象,筛选出1株产生蛋白酶和纤维素酶的菌株,经过反接种验证,该菌能较好地发酵豆渣,形成与霉豆渣相似的质地和风味。以菌株在PDA上的菌落特征、菌丝形态、产孢结构及其孢子形态等的鉴定为基础,结合ITS序列分析,确定该株菌为粗糙脉孢菌。采用菌丝体生长量干重为主要衡量指标进行菌种生理特性研究,确定该菌对数生长期在0~36 h,最适生长温度为28~32℃,最适生长pH值为6。

A Meidouzha production strain was selected from traditional fermentative soybean residue. It could produce protease and cellulase. After vaccination,the strain could fermented soybean residue well,and then develop the similar texture and flavor to the Meidouzha. The strain was identified by their morphs of mycerial,hyphal,spore and ITS sequence. The results showed that the strains were Neurospora crassa. Then the physiological property of the strain was studied based on different pH value,temperature,time and weight of the mycelium to define optimum phase of growth of the strain. The range of its log growth phase was 0 ~ 36 h. The range of optimum growth temperature was 28 ~ 32℃. The optimum pH was 6.