为提高新疆冰川细菌Serratia sp.A29-2的低温蛋白酶产酶活性,在单因素试验的基础上,通过正交试验和响应面试验,对其发酵培养基和发酵条件进行了优化。研究表明:其最佳培养基组成为蛋白胨15.0 g/L、酵母粉5.0 g/L、麦芽糖10.0 g/L、CaCl20.5 g/L。最优发酵条件是:温度22℃,pH值7.0,时间60 h,NaCl质量分数1.0%。以优化培养基为基础,在最适发酵条件下,菌株Serratia sp.A29-2产低温蛋白酶酶活可达54.25 U/mL,是初始酶活的1.93倍。

To improve the low temperature protease activity of Serratia sp. A29-2 isolated from Xinjiang glaciers, the fermentation medium and fermentation conditions of Serratia sp. A29-2 were optimized respectively through orthogonal experiment and response surface test on the basis of single factor test. The results showed that the optimum culture media contained peptone 15. 0g / L,yeast extract 5. 0g / L,maltose 10 g / L,CaCl20. 5g / L. The optimum fermentation conditions were temperature 23℃,pH 7. 0,fermentation time 60h,mass fraction of NaCl 1. 0%. Under the optimum conditions,the low temperature protease activity produced by Serratia sp. A29-2 reached 54. 25U / mL,which was 1. 93 times higher than that under the condition before optimization.