研究了不同提取剂和提取条件对红曲霉玉米粉豆渣发酵液中Monacolin K的超声提取效果。实验以双波长紫外分光光度法检测发酵液中的Monacolin K浓度为指标,在单因素实验的基础上,通过正交实验及分析,确定超声提取工艺条件。实验结果表明:最佳提取剂为75%的乙醇,提取时间40 min,提取温度60℃,料水比(菌丝湿重∶75%乙醇)为1∶8(g∶mL),超声功率250 W。在此条件下,Monacolin K的提取率达到93.6μg/mL。

The effect of extractants and extraction conditions on the ultrasonic extraction rates of Monacolin K in fermentation broth of Monascus were studied. The optimal conditions were determined by orthogonal test and the Monacolin K in fermentation broth detected by dual wavelength ultraviolet spectrophotometry was chosen as the estimated index. The results indicated that the best extractant was ethanol and the optimal conditions were as follows: ultrasonic power was 250W,material liquid ratio( mycelial wet weight / g ∶ 75% ethanol / mL) was 1 ∶ 8,extracting time was 40min,extracting temperature was 60 ℃ and ethanol concentration was 75%. Under these conditions,the extraction ratio of Monacolin K was 93. 6 μg / mL. The extraction process is a viable method for extracting Monacolin K from fermentation broth of Monascus.