研究大孔吸附树脂对苹果多酚的纯化工艺。从6种大孔树脂中筛选出吸附性能和解吸性能良好的XAD7HP型大孔吸附树脂。在单因素试验基础上,设计正交实验,对大孔树脂纯化苹果多酚工艺进行优化。结果表明:最佳纯化条件为上样浓度1.46 mg/mL、上样pH值5.50、上样速度1.25 mL/min,洗脱剂种类为丙酮、洗脱剂浓度为90%、洗脱速度为1 mL/min。在此条件下,纯化后纯度高达80.1%,比纯化前提高了10倍多,且回收率高达90%。纯化后苹果多酚进行高效液相色谱分析,大部分杂质峰不再出现,检测出5种多酚成分。

Purification process of Macroporous resin for apple polyphenols was studied. XAD7HP macroporous resin showed the best adsorption and desorption capacity among six kinds of macroporous resins. On the basis of single factor optimization,an orthogonal experiment was designed to optimize the purification process. Results showed that the optimal purification conditions were: sample concentration 1. 46 mg /ml,sample pH 5. 50,the flow rate of sample1. 25 ml /min,eluant type 90% acetone,eluent flow rate 1 ml /min. Under the above conditions,purity was up to80. 1%,10 times more than before. The recovery rate was as high as 90%. Purified Apple polyphenols were analyzed by High Performance Liquid Chromatography. Five kinds of polyphenols were detected and most of the impurity peaks were disappeared. This method can effectively purify the apple polyphenols and provide reference for large-scale industrial production of apple polyphenols.