以正己烷为溶剂,大豆卵磷脂(phosphatidylcholine,PC)为原料,利用Lipozyme RM IM催化乙醇解反应制备溶血卵磷脂(LPC),通过单因素实验确定了适宜的反应条件:温度40℃,加酶量15%(以PC质量计),溶剂比1∶2(PC:正己烷,g∶mL),底物比1∶3(PC∶醇中羟基,mol∶mol),加水量1∶80(PC:水,g∶μL);在此条件下,反应16 h达到平衡,LPC转化率达93%。在此基础上,考察其他醇对卵磷脂乙醇解的影响,发现正丁醇和叔丁醇对PC乙醇解无明显地促进或抑制作用,1,2-丙二醇和丙三醇对PC乙醇解表现出抑制作用。

Lysophosphatidylcholine( LPC) was prepared through alcoholysis of soybean phosphatidylcholine( PC) catalyzed by Lipozyme RM IM in n-hexane. Based on single factor experiments,the optimal ethanolysis conditions were obtained as followed: reaction temperature 40℃,lipase dosage 15%( in mass of PC),solvent ratio 1∶ 2( PC∶ n-hexane,m/v),substrate ratio 1∶3( PC∶ hydroxyl of alcohol,mol/mol),the amount of water 1∶80( PC∶H2O,g /uL). Under these conditions,the conversion of LPC was 93% after 16 h. Based on these optimal conditions,the effect of other alcohols on the ethanolysis of PC was studied. n-butanol and tert-butanol showed no significant changes on ethanolysis,while 1,2-propylene glycol and glycerol showed significant inhibiting on ethanolysis.