文中以VC、BHT和EDTA为对照,通过总抗氧化能力、DPPH自由基清除活性、ABTS自由基清除活性、铁离子还原能力和金属离子螯合能力等5种体系对咖啡酸、芦丁、白藜芦醇、根皮素和根皮苷等天然多酚类化合物的抗氧化活性进行了综合评价。结果表明:咖啡酸的总抗氧化能力、DPPH自由基清除能力[IC50(0.023±0.004)mg/mL]和铁离子还原能力均为最高,在浓度为0.1 mg/mL时总抗氧化能力达到(1.873±0.022)mg VC eq/mg;白藜芦醇的ABTS自由基清除能力最强[IC50(0.056±0.006)mg/mL],极显著高于阳性对照BHT和VC(P<0.01);芦丁的金属离子螯合能力最强,白藜芦醇次之,其他多酚类化合物不具备金属离子螯合能力。咖啡酸、芦丁、白藜芦醇、根皮素和根皮苷均具有较强的抗氧化活性,是潜在的天然抗氧化剂。

The antioxidant activity of caffeic acid,rutin,resveratrol,phloretin,and phlorizin were evaluated compared with VC,BHT and EDTA. Five areas were tested: the total antioxidant capacity,DPPH free radicals scavenging activity,ABTS free radicals scavenging activity,ferric reducing ability and ferrous ions chelating activity. The results indicated that caffeic acid showed strong total antioxidant capacity,DPPH radical scavenging activity( IC500. 023 ± 0. 004 mg /mL) and ferric reducing ability,the total antioxidant capacity reached 1. 873 ± 0. 022 mg VCeq /mg at a concentration of 0. 1 mg /mL; Resveratrol showed a strong ABTS radical scavenging activity( IC500. 056 ±0. 006 mg /mL),significantly higher than that of BHT and VC( P < 0.01). Rutin showed the strongest ferrous ions chelating activity,followed by resveratrol. Other phenolic compounds showed little ferrous ions chelating activity. All the above tests showed 5 compounds have the potential natural antioxidants.