以610 nm透光率、蛋白去除率、多糖去除率、过滤速度等为指标,从8种不同类型的絮凝剂中筛选出适合的絮凝剂,通过HPLC分析比较了2种絮凝剂对发酵液中小分子代谢产物提取的影响,并探讨了所筛选絮凝剂的使用条件。比较了4种固液分离方法对细菌发酵液的分离效果,进而确定了适合海洋细菌发酵液快速预处理的工艺。结果表明:Al2(SO4)3耦合膜超滤法絮凝速度快、蛋白多糖去除率高、滤液澄清度好,适用于大部分海洋细菌发酵液的预处理。

The optimum flocculation were screened from the different kinds of flocculations through comparison of610nm light-transmittance,the filtration rate,the removal ratio of protein and polysaccharose. Using Al2( SO4)3and poly-AlCl3 as flocculation,their effects on the extraction of the secondary metabolites from marine bacterias were investigated by HPLC( high-performance-liquid-chromatograhy). The effects of four kinds of solid-liquid separation ways on the fermentation borth of marine bacteria were compared. Results showed that the coupling method of flocculation and membrane ultrafiltration was adaptable to highly active and rapid pretreatment of different types of marine bacteria.