为了探索诱导植物乳杆菌生物膜形成的外界环境因素,采用微量板半定量法分别对不同理化因素及几种食品添加剂诱导其生物膜形成情况进行了探索。结果显示,6%的NaCl能够明显诱导植物乳杆菌形成强阳性生物膜;弱酸性的条件(pH 4.8~5.8)、0.2%和0.4%的柠檬酸、H3PO4以及明胶的添加均能够诱导植物乳杆菌形成弱阳性的生物膜。植物乳杆菌生物膜的形成受到某些外界环境因素的调控。

As one kind of important lactic acid bacteria,Lactobacillus plantarum has attracted more and more attentions in the fields of food fermentation,industrial malolactic fermentation and healthcare. Semi-quantitative assay was used to explore the environmental factors which induced the biofilm formation by L. plantarum. Several different physical and chemical factors,and food additives were investigated and the results showed that strong positive biofilm formation by L. plantarum was obviously induced by 6% sodium chloride. Weak positive biofilm formation was induced by the weak acidic( pH 4. 8 ~ 5. 8) conditions,citrate and phosphate at the concentrations of 0. 2% and0. 4%,respectively,and the addition of gelatin. In conclusion,the biofilm formation by L. plantarum was greatly affected by some environmental factors.