光滑球拟酵母是重要的丙酮酸工业生产菌株,主要利用葡萄糖作为碳源生产丙酮酸。该研究以产量大且价格便宜的甘油部分替代葡萄糖并控制碳源组成为80 g/L葡萄糖和20 g/L甘油,摇瓶条件下发酵52 h,丙酮酸产量达到27.6 g/L,在7 L发酵罐上最终丙酮酸产量为61.7 g/L,与碳源为100 g/L葡萄糖的对照条件相比,几乎没有任何变化。由于碳源是培养基的主要成分,用甘油替代葡萄糖可以降低丙酮酸生产的原料成本,减轻由于大量生物柴油合成造成的甘油产能过剩问题。

Torulopsis glabrata is an important strain for pyruvate production on industrial scale. It mainly utilized glucose as carbon source in previous reports. In the present study,the inexpensive bulk raw material glycerol was used to partially replace the glucose as carbon source. When the medium was supplemented with 80 g /L glucose and20 g /L glycerol,the pyruvate production was 27. 6 g /L in flask culture and 61. 7 g /L in a 7 L fermentor,respectively. Compared to the case without glycerol addition,no obvious change was detected on pyruvate production. Since carbon source was the main component of the medium,it decreased the cost of raw materials during pyruvate accumulation and reduced the problem of excessive production of glycerol by partially replacement of glucose.