采用与健康人体类似条件的体外胆固醇氧化模型,研究2种葡萄酒对自由基引发的胆固醇氧化的抑制能力,并分析了葡萄酒中主要抗氧化物质的含量。实验结果表明,2种葡萄酒对胆固醇的氧化具有明显的抑制作用,桂葡萄1号葡萄酒的抑制能力高于刺葡萄酒;在葡萄酒与胆固醇氧化模型的体积比为1∶50,1∶100,1∶200时,反应24 h桂葡1号葡萄酒对胆固醇的抑制率分别为98.4%、97.2%、80.9%,刺葡萄酒的抑制率分别为96.6%、92.9%、72.8%;桂葡1号葡萄酒和刺葡萄酒中的游离花色苷、原花青素的含量分别达到759.9、403.1和712.6、465.0 mg/L,白黎芦醇的含量为5.62和4.25 mg/L;富含多酚类抗氧化物质是南方山葡萄酒抗胆固醇氧化的物质基础。

Capabilities in inhibiting cholesterol oxidation of southern wild grape wines caused by free radical in vitro were studied using a cholesterol oxidation emulsion system similar to healthy body similar conditions,and the content of the main antioxidants in wines were analyzed. The results showed that Gui-Pu No. 1 and brier grape wines could inhibit cholesterol oxidation obviously. The inhibition ability of Gui-Pu No. 1 wine was higher than brier grape wine. When wines were added into the cholesterol oxidation emulsion system at a ratio of 1∶ 50,1∶ 100,1∶ 200( v /v),the inhibition ratio of Gui-pu No. 1 after 24 h reaction were 98. 4%,97. 2%,80. 9%,respectively,and those of the brier grape wine were 96. 6%,92. 9%,72. 8%. The contents of free anthocyanins and proanthocyanidin in Gui-Pu No. 1 and brier grape wine reached 759. 9,403. 1mg /L and 712. 6,465. 0 mg /L respectively,and the contents of resveratrol were 5. 62 mg /L and 4. 25 mg /L. Rich polyphenol antioxidants in southern wild grape wines were the material basis to inhibit cholesterol oxidation.