为探讨发酵肉制品中生物胺的产生、累积机理,采用高效液相法分析了新疆熏马肠样品产生物胺优势菌株克雷伯氏菌(Klebsiella sp.)、阴沟肠杆菌(Enterobacter cloacae strain)和成团泛菌(Pantoea agglomerans strain)产生物胺能力。并选取克雷伯氏菌、阴沟肠杆菌、成团泛菌作为群体感应现象研究对象,利用微生物传感菌Agrobacterium tumefaciens A136及Agrobacterium tumefaciens KYC55平行线法检测AHLs。检测表明,3株产生物胺菌能够发生群体感应现象。通过β-半乳糖苷酶活性检测法测定AHLs活性试验、HPLC法对产生物胺菌株AHLs定性分析,结果表明,β-半乳糖苷酶活性越高,产生物胺菌株分泌的AHLs越多;Klebsiella sp.、Enterobacter cloacae strain均能分泌:N-butanoyl-L-homoserine lactone(C4-HSL)、N-hexanoyl-DL-homoserine homoserine lactone(C6-HSL)、N-octanoyl-DL-homoserine lactone(C8-HSL)类型的信号分子。

This work attempts to examine biogenic amine of Klebsiella sp.,Enterobacter cloacae strain,Pantoea agglomerans strain by high performance liquid chromatography. The screened strains were isolated from Xinjiang Smoked horse intestine samples. Quorum sensing is a cell-to-cell signaling system that functions by means of diffusible autoinducer( AI) signal molecules that allow the regulation of cellular processes in a manner that depends on the density of the microbial population. QS is a common development group behavior,which bacteria use signaling molecules produced by monitoring their own population density changes,regulation of gene expression,collaborative adapt to the environment. The parallel line experiment of Agrobacterium tumefaciens A136 and Agrobacterium tumefaciens KYC55,the results show that the biogenic amines strains can occurred blue change by X-gal. According to autoinducer activity test found that if the β-galactosidase activity of quorum sensing strains is higher,the more the bacteria secrete AHLs.AHLs were different secreted in different period of quorum sensing bacterial. Analysis of AHLs types of these strains by HPLC. Klebsiella sp. and Enterobacter cloacae strain can secrete signaling moleculesC4-HSL,C6-HSL,C8-HSL. Analysis of quorum sensing of biogenic amine strains,in order to establish basic theory about biogenic amine. It provides a good theoretical basis for the industrial production of fermented meat and security.