为优化NaCl溶液提取卵黄高磷蛋白的工艺条件,提高提取率,以卵黄高磷蛋白的得率为指标,选用Box-Behnken设计,采用4因素3水平的响应面分析法,探讨了洗涤次数、液料比、提取时间、NaCl浓度对卵黄高磷蛋白得率的影响。结果表明:各因素对卵黄高磷蛋白得率的影响的主次顺序为,洗涤次数>液料比>提取时间>NaCl浓度,卵黄高磷蛋白得率的理论极值为5.47%。卵黄高磷蛋白的最佳提取工艺参数为:NaCl浓度12%,提取时间5 h/次,洗涤次数5次,液料比(g∶mL)12∶1;在此条件下,卵黄高磷蛋白的得率为5.27%,提取率为88.16%。

To optimize the processing of phosvitin extraction from the delipidated duck egg yolk protein powder and increase the extraction rate,the response surface methodology with four factors at three levels was adopted. By measuring the yield of phosvitin,the effect of sodium chloride concentration,ratio of liquid to solid,extraction time,and the washing time on the yield of phosvitin was investigated. The experimental results showed: the order of various factors affecting the yield of phosvitin was: washing time > ratio of liquid to solid > extraction time > sodium chloride concentration. The theoretical maximum yield of phosvitin was 5. 47%. the optimum conditions,which were suitable for extracting phosvitin by using sodium chloride solution,were as follows: sodium chloride concentration of 12%,extraction time 5h,ratio of liquid to solid of 12: 1,5 times of washing. Under the above optimal extraction conditions,the yield of phosvitin was 5. 27% and the extraction rate was 88. 16%.