研究海地瓜胶原蛋白的水法提取条件,以提高胶原蛋白得率。以海地瓜为原料,以粗提液中胶原蛋白提取率为衡量指标,选取提取温度、液料比、pH值、提取时间作为主要影响因素,在单因素试验基础上运用响应面分析法(RSM)得到拟合回归方程,确定海地瓜胶原蛋白最佳提取工艺。实验结果表明:海地瓜胶原蛋白水提法的最佳条件为,温度86℃,液料比(mL∶g)13∶1,pH 7.6,提取时间21h。在该条件下,胶原蛋白提取率模型预测值为37.26%,实测验证值36.68%,与预测值相符。

Acaudinaleucoproctaas was used in the study as raw material and the conditions of its extracting were optimized through single factor experiment and response surface methodology(RSM).The optimal conditions of Acaudinaleucoprocta collagen extraction were obtained based on the analysis of significant factors and their interactions.The results are as following:extraction temperature 80℃,water /solid ratio 13 /1,PH 7.6,extraction time 21 h.The extraction rate was 36.68%,and is fit with the prediction model.Water-extraction method did not only produce pure collagen,but also had no heavy metal residues.The study provided a reference for the development of Acaudinaleucoprocta.