分别控制5.0 m3/h、3.5 m3/h两种通气水平下进行L-色氨酸分批发酵,考察了两种通气水平下谷氨酸棒杆菌HYH3-1发酵过程参数变化。实验结果表明,当通气量为5.0 m3/h时pH变化幅度较大,发酵终止时的pH高于通气量为3.5 m3/h的发酵液pH。在通气量较大的条件下,发酵后期发酵液中的溶氧保持在较高的浓度,对DO有显著影响。当通气量为5.0 m3/h时,发酵液中色氨酸产量和细胞干重分别为43.3 g/L和18.3 g/L,发酵过程残糖变化不明显。

Corynebacterium glutamicum was cultivated in two parallel 10 L fermenters under 5.0 m3/h and 3.5m3/h ventilation,respectively.The fermentation conditions of batch cultivation were investigated systematically.The experiment result showed that pH value decreased sharply when the ventilation was 5.0 m3/h.pH value was higher under 5.0 m3/h of ventilation than that under 3.5 m3/h at the end of the fermentation.High concentration of dissolved oxygen fermentation anaphase in fermentation liquid had a significant influence on DO.The L-tryptophan yield and the weight of dried cells reached 43.3 g /L and 18.3 g /L while the ventilation was 5.0 m3/h.Fermentation residual sugar did not change significantly.