以红法夫酵母野生型菌株(Phaffia rhodozyma 02)为原始出发菌株,依次进行2轮紫外(UV)-氯化锂(LiCl)和60Co-γ射线诱变,以40μmol/L二苯胺为抗性筛选剂,筛选得到1株遗传稳定的高产虾青素红法夫酵母突变菌株H4-4,命名为HSC-125,虾青素产量为25.36 mg/L,是原始出发菌株P.rhodozyma 02(4.75 mg/L)的5.34倍。采用2轮UV-LiCl和60Co-γ射线复合诱变结合二苯胺作为抗性筛选剂,选育得到的高产虾青素的红法夫酵母突变株,其遗传性质稳定,展现良好效果。

Herein,Phaffia rhodozyma wild-type strain(P.rhodozyma 02) was used as original strain to carry out two rounds of ultraviolet(UV)-LiCl and60Co-γ mutagenesis breeding successively.By using YM medium with 40μmol/L diphenylamine as screening agent,an astaxanthin high-yielding P.rhodozyma mutant strain H4-4 was screened,which was genetically stable and named as HSC-125.The volumetric productivity of astaxanthin of H4-4was 25.36 mg /L,which was 5.34 times higher than P.rhodozyma 02(4.75 mg /L).Two rounds of UV-LiCl and60Co-γ mutagenesis with diphenylamine as selection factor showed good results of breeding high astaxanthin-producing and genetically stable phaffia rhodozyma strains.