采用截留分子质量(MWCO)为1000 Da、10 kDa和30 kDa的卷式膜,探索卷式膜超滤核黄素发酵液的可行性。对比分析超滤膜的杂质截留率和膜通量,发现MWCO为10 kDa的卷式超滤膜对核黄素具有较好的分离效果。通过研究操作压力、温度、流速及浓缩倍数对膜通量的影响,确定核黄素发酵液超滤的最佳条件为:温度30℃,流速5 m/s,操作压力0.2 MPa,浓缩倍数为3.8倍。超滤后,经过氧化结晶核黄素的纯度可达98.6%,总回收率达95%。

A feasibility study on extracting riboflavin from fermentation by spiral-wound membranes with molecular weight cut-offs(MWCO) of 1000 Da,10 kDa and 30 kDa was explored.The membrane with MWCO of 10 kDa was selected to have the best separation effect through comparing the efficiency and flux of the membrane.After the suitable membrane was ascertained,the effects of various parameters for operation such as pressure,flow rate,temperature,concentration factor were studied.The optimal operating conditions were obtained as follows:operation pressure0.2 MPa,operation temperature 30 ℃,flow rate 5 m /s,concentration factor 3.8.The riboflavin purity could reach98.6 % and total recovery rate was up to 97% after ultrafiltration and crystallization