以香菇子实体为材料,依次经过0.9%Na Cl和80℃热水进行提取,获得多糖LFI和LFII,然后比较了树脂AB-8和DA201对LFI和LFII的脱除色素效果,确定了AB-8为最佳脱色树脂,脱除色素率分别为72%和53%。用多角度光散射仪器对所分离多糖的分子质量进行了测定,然后利用红外光谱,1H-NMR,13C-NMR和甲基化分析分析对LFI和LFII进行结构表征。得到实验结果:LFI和LFII的重均分子质量Mw分别为1.350×106Da和2.771×106Da,数均分子质量Mn分别为1.337×106Da和2.693×106Da。LFI多糖组分由α-1Man、β-1Glc、α-1Gal、β-1,3-Glc-、α-1,6-Glc-、α-1,4-Glc-和α-1,4,6-Man-糖单元组成;LFII多糖组份由α-1Xyl,α-1Gal,β-1Glc,β-1,3-Glc-,β-1,6-Glc-,α-1,6-Man-,β-1,3,6-Glc,α-1,4,6-Man-和α-1,2,6-Gal-糖单元组成。由此获得并实现了香菇子实体中可溶性多糖的结构信息。

Two water-souble polysaccharides,named LFI and LFII,were isolated from Lentinus edodes by 0. 9%Na Cl and hot water extraction,respectively. Three resins named AB-8,DA201 and JK008 were used to remove the pigments from LFI and LFII,wherein AB-8 was more suitable for removing the pigments with removal rate of 72% and53% from LFI and LFII respectively. The Mw of LFI and LFII was 1. 350 × 106 Da and 2. 771 × 106 Da,the Mn of LFI and LFII was 1. 337 × 106 Da and 2. 693 × 106 Da. IR,13C-NMR and1H-NMR studies indicated that LFI consisted of α-1Man,β-1Glc,α-1Gal,β-1,3-Glc-,α-1,6-Glc-,α-1,4-Glc- and α-1,4,6-Man6- residues,while LFII was found to contain α-1Xyl,α-1Gal,β-1Glc,β-1,3-Glc-,β-1,6-Glc-,α-1,6-Man-,β-1,3,6-Glc,α-1,4,6-Man- and α-1,2,6-Gal- residues.