应用响应面法设计对Gluconobacter kondonii静息细胞转化合成L-赤藓酮糖进行优化。在单因素试验基础上,利用响应面分析法(RSM)对转化条件进行优化,并建立了各因素与L-赤藓酮糖之间的数学模型。结果表明,静息细胞浓度、p H值、赤藓糖醇浓度、反应时间以及温度对L-赤藓酮糖产量均有不同程度影响。响应面预测产L-赤藓酮糖的最佳工艺为静息细胞浓度1.65%、赤藓糖醇浓度97.9 g/L、温度30.2℃、p H值为5,反应时间18 h,此时L-赤藓酮糖产量为96.1 g/L,与响应面极值相吻合,转化率为98.2%,生产强度达到5.34 g/(L·h),表明优化方案达到了预期效果。

The production of L-erythrulose by resting cells of Gluconobacter kondonii was optimized using response surface design. On the basis of single factor test,the Response Surface Methodology( RSM) was designed for the first time,and the mathematical model was set up between various factors and L-erythrulose. The results indicated that the effects of the concentration of resting cells and erythritol,p H value,reaction time and temperature on the production of L-erythrulose were significant. Response Surface prediction showed that the optimal conditions for production of Lerythrulose were as follows: the concentration of resting cells 1. 65%,the concentration of erythritol 97. 9 g / L,temperature 30. 2℃,p H value 5 and the reaction time 18 h. Under these conditions,the yield of L-erythrulose reached96. 1 g / L,and the response surface extremes coincide conversion was 98. 2%,production intensity was 5. 34 g /( L·h). All of these showed that optimization program achieved the desired effect.