建立了同时检测水产品中丁香酚、异丁香酚、甲基丁香酚、甲基异丁香酚、乙酰基异丁香酚5种丁香酚类麻醉剂残留量的高效液相色谱方法。样品经乙腈超声提取,正己烷重复脱脂后,采用高效液相色谱进行分析,外标法定量。5种丁香酚类麻醉剂在0.05~10.00μg/m L内,线性关系良好,相关系数均高于0.999 0。在添加浓度为0.10~1.00 mg/kg时,平均回收率在80.76%~102.63%,相对标准偏差为0.25%~5.49%。方法的定量限(LOQ)丁香酚、异丁香酚和甲基异丁香酚为0.05 mg/kg,甲基丁香酚、乙酰基异丁香酚为0.10 mg/kg。所建立的方法适合于批量水产品中丁香酚类麻醉剂的残留检测。

A high performance liquid chromatography( HPLC) method has been developed for the detection of five eugenolanesthetic residuessimultaneous: Eugenol,Iso-eugenol,Methyl eugenol,Methyl iso-eugenol,Acetyl iso-eugenol in aquatic products. Sample was ultrasonic extracted by acetonitrile,the fat was cleaned up twice by n-hexane,and then the diluted solution was determined by HPLC. External standard calibration was used in the determination.Five kinds of eugenol type of anesthetic showed good linearity in the concentrations range of 0. 05 ~ 10. 00 μg / m L in the standard solutions carves,and all of the correlation coefficents( R2) were higher than 0. 999 0. At the spiked levels of 0. 10 ~ 1. 00 mg / kg,the average recoveries were 80. 76% ~ 102. 63%. The relative standard deviations were0. 25% ~ 5. 49%. The limits of quantificationg( LOQ) of Eugenol,Iso-eugenol and Methyl iso-eugeno were 0. 05 mg /kg,and Methyl eugenol and Acetyl iso-eugenol were 0. 10 mg / kg. The method is suitable for the detection of batch clove phenol drug residues in aquatic products.