研究不同益生元对植物乳杆菌在不同培养条件下的促增殖作用。选用5株生理特性不同的植物乳杆菌为研究对象,利用Bioscreen检测它们的生长情况,进一步通过Baranyi模型拟合曲线,从而得到最大比生长速率和迟滞期。结果表明:在MRS培养基下,添加5种不同的益生元对植物乳杆菌无明显促增殖作用;在以益生元替代葡萄糖作为碳源的MRS培养基中,植物乳杆菌对不同的益生元具有选择偏好性,且具有菌株特异性;在模拟肠道体系下,与对照相比,益生元能明显促进所有植物乳杆菌的增长,其中低聚果糖与低聚半乳糖最优。实验结论显示,模拟肠道体系可以作为最精确和快速的益生元筛选体系。
To study the effects of different prebiotics on the proliferation of Lactobacillus plantarum in different culture conditions, five strains of L. plantarum with different physiological characteristics were used as the research objects. The growth was detected with Bioscreen, and the maximum specific growth rate and lag period were obtained by Baranyi models. The results showed that, in MRS medium, adding five different prebiotics had no significant effect on the proliferation of L. plantarum; in MRS medium with prebiotics instead of glucose as carbon source, L. plantarum had preference for different prebiotics, and has strain specificity; in the simulated intestinal system, prebiotics can significantly promote the growth of all L. plantarum, among which fructo-oligosaccharide and galacto-oligosaccharide were the best. The simulated intestinal system was proved to be a precise and fast system for prebiotics screening.
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