研究报告

戴尔有孢圆酵母对品丽珠和马瑟兰葡萄酒香气物质的影响

  • 宿萌 ,
  • 李蕊蕊 ,
  • 王欢 ,
  • 肖冬光 ,
  • 郭学武 ,
  • 陈叶福
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  • (天津科技大学 生物工程学院,工业发酵微生物教育部重点实验室,天津,300457)
硕士研究生(陈叶福教授为通讯作者,E-mail:yfchen@tust.edu.cn)

收稿日期: 2020-04-06

  修回日期: 2020-05-14

  网络出版日期: 2020-12-11

基金资助

国家自然科学基金资助项目(31671843)

Effects of Torulaspora delbrueckii on the aroma compounds of Cabernet Franc and Marselan wines

  • SU Meng ,
  • LI Ruirui ,
  • WANG Huan ,
  • XIAO Dongguang ,
  • GUO Xuewu ,
  • CHEN Yefu
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  • (College of Biotechnology, Tianjin University of Science and Technology, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin 300457, China)

Received date: 2020-04-06

  Revised date: 2020-05-14

  Online published: 2020-12-11

摘要

分别选用酿酒酵母、戴尔有孢圆酵母单独发酵以及两者顺序接种的混合发酵,采用顶空固相微萃取结合气相色谱-质谱联用技术(headspace solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)探究戴尔有孢圆酵母对品丽珠和马瑟兰葡萄酒香气的影响。结果表明,戴尔有孢圆酵母单独发酵以及其参与的混合发酵均可降低葡萄酒中滴定酸的含量,且有利于苯乙醇、脂肪酸乙酯(辛酸乙酯、癸酸乙酯和月桂酸乙酯)的合成,增加了葡萄酒的花香和果香。不同葡萄品种酒样中香气物质的种类和质量浓度各不相同,马瑟兰在葡萄酒中的香气更加浓郁,除了能够产生丰富的酯类化合物,还检测出α-紫罗兰酮和β-大马酮,其他萜烯类化合物的含量也有显著提高,使葡萄酒中花香、果香气味更加显著。

本文引用格式

宿萌 , 李蕊蕊 , 王欢 , 肖冬光 , 郭学武 , 陈叶福 . 戴尔有孢圆酵母对品丽珠和马瑟兰葡萄酒香气物质的影响[J]. 食品与发酵工业, 2020 , 46(21) : 62 -69 . DOI: 10.13995/j.cnki.11-1802/ts.024153

Abstract

To study the effects of Torulaspora delbrueckii on the aroma of Cabernet Franc and Marselan wines, Saccharomyces cerevisiae and T. delbrueckii were used respectively or in sequential combination. The components were analyzed by using solid phase microextraction combined with gas chromatography-mass spectrometry. The results showed that in wines fermented with T. delbrueckii alone or both yeasts, the contents of titratable acids were reduced and the synthesis of phenyl ethanol and fatty acid ethyl esters (ethyl octanoate, ethyl decanoate and ethyl laurate) were elevated, thus greatly increased the floral and fruity aroma of the wines. Different varieties and concentrations of aroma compounds were detected in wines made from different grapes, while most abundant aroma compounds were detected in Marselan wine. Besides rich esters, α-ionone and β-damascenone were detected, and the other terpenoids were also increased obviously, which made the floral and fruity aroma of Marselan wine more significant.

参考文献

[1] 卜光明,周化斌,周茂洪,等.酿造酒中非酿酒酵母的研究进展[J].食品工业科技,2019,40(14):346-352.
[2] JOLLY N P,AUGUSTYN O P H,PRETORIUS I S.The role and use of non-Saccharomyces yeasts in wine production[J].South African Journal of Enology and Viticulture,2006,27(1):15-39.
[3] MA D C,YAN X,WANG Q Q,et al.Performance of selected P.fermentans and its excellular enzyme in co-inoculation with S. cerevisiae for wine aroma enhancement[J].LWT-Food Science and Technology,2017,86:361-370.
[4] 李旋,亓桂梅,王超萍.非酿酒酵母在葡萄酒生产中的作用及研究进展[J].中外葡萄与葡萄酒,2019(1):52-56.
[5] 于洋,粟春燕,陈晶瑜.3株葡萄酒非酿酒酵母的生长与发酵特性[J].食品科学,2015,36(19):106-111.
[6] CABAROGLU T,SELLI S,CANBAS A,et al.Wine flavor enhancement through the use of exogenous fungal glycosidases[J].Enzyme and Microbial Technology,2003,33(5):581-587.
[7] PADILLA B,GIL J V,MANZANARES P.Past and future of non-Saccharomyces yeasts:from spoilage microorganisms to biotechnological tools for improving wine aroma complexity[J].Frontiers in Microbiology,2016,7:411.
[8] RENAULT P,COULON J,DE REVEl G,et al.Increase of fruity aroma during mixed T. delbrueckii/S. cerevisiae wine fermentation is linked to specific esters enhancement[J].International Journal of Food Microbiology,2015,207:40-48.
[9] 原苗苗,姜凯凯,孙玉霞,等.戴尔有孢圆酵母对葡萄酒香气的影响[J].食品科学,2018,39(4):99-105.
[10] CIANI M,COMITINI F,MANNAZZU I,et al.Controlled mixed culture fermentation:A new perspective on the use of non-Saccharomyces yeasts in winemaking[J].FEMS Yeast Research,2010,10(2):123-133.
[11] SUN S Y,GONG H H,JIANG X M,et al.Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae on alcoholic fermentation behaviour and wine aroma of cherry wines[J].Food Microbiology,2014,44:15-23.
[12] ZHANG B Q,LUAN Y,DUAN C Q,et al.Use of Torulaspora delbrueckii co-fermentation with two Saccharomyces cerevisiae strains with different aromatic characteristic to improve the diversity of red wine aroma profile[J].Frontiers in Microbiology,2018,9:606.
[13] 刘灿珍,董书甲,姜凯凯,等.5株非酿酒酵母的耐受性及发酵特性研究[J].中国酿造,2017,36(10):42-46.
[14] CHEN D,YAP Z Y,LIU S Q.Evaluation of the performance of Torulaspora delbrueckii,Williopsis saturnus,and Kluyveromyces lactis in lychee wine fermentation[J].International Journal of Food Microbiology,2015,206:45-50.
[15] 杨诗妮,叶冬青,贾红帅,等.本土戴尔有孢圆酵母在葡萄酒酿造中的应用潜力[J].食品科学,2019,40(18):108-115.
[16] 荆雄,杨辉,苏文,等.非酿酒酵母与酿酒酵母混合发酵柿子酒特性的研究[J].中国酿造,2018,37(12):52-56.
[17] 谭玉岩,郝宁.酿酒酵母与非酿酒酵母混合发酵对果酒品质的影响[J].食品工业科技,2020,41(8):353-359.
[18] BELDA I,NAVASCUÉS E,MARQUINA D,et al.Dynamic analysis of physiological properties of Torulaspora delbrueckii in wine fermentations and its incidence on wine quality[J].Applied Microbiology and Biotechnology,2015,99(4):1 911-1 922.
[19] BASSO R F,ALCARDE A R,PORTUGAL C B.Could non-Saccharomyces yeasts contribute on innovative brewing fermentations?[J].Food Research International,2016,86:112-120.
[20] CIANI M,COMITINI F. Red wine technology,chapter 4:Use of non-Saccharomyces yeasts in red winemaking[M].Academic Press,2019:51-68.
[21] TOMAŠEVIĆ M,ĆURKO N,GRACIN L,et al.Analysis of aroma of white wine (Vitis vinifera L. Poip) by gas chromatography-mass spectrometry[J].Croatian Journal of Food Technology Biotechnology & Nutrition,2016,11(3-4):122-127.
[22] HAUSCH B J,LORJAROENPHON Y,CADWALLADER K R.Flavor chemistry of lemon-lime carbonated beverages[J].Journal of Agricultural and Food Chemistry,2015,63(1):112-119.
[23] RAPP A,VERSINI G.Influence of nitrogen compounds in grapes on aroma compounds of wines[J].Developments in Food Science,1997,37(06):71-82.
[24] 成冰,张京芳,王月晖,等.酿酒白葡萄氨基酸组成特征分析[J].食品科学,2013,34(20):174-177.
[25] VIANA F,GIL J V,GENOVÉS S,et al.Rational selection of non-Saccharomyces wine yeasts for mixed starters based on ester formation and enological traits[J].Food Microbiology,2008,25(6):778-785.
[26] SCHREIER P,JENNINGS W G.Flavor composition of wines:A review[J].Critical Reviews in Food Science & Nutrition,1979,12(1):59-111.
[27] 宋茹茹,段卫朋,祝霞,等.戴尔有孢圆酵母与酿酒酵母顺序接种发酵对干红葡萄酒香气的影响[J].食品与发酵工业,2019,45(24):1-9.
[28] SWIEGERS J H,PRETORIUS I S.Yeast modulation of wine flavor[J].Advances in Applied Microbiology,2005,57:131-175.
[29] AZZOLINI M,FEDRIZZI B,TOSI E,et al.Effects of Torulaspora delbrueckii and Saccharomyces cerevisiae mixed cultures on fermentation and aroma of Amarone wine[J].European Food Research and Technology,2012,235(2):303-313.
[30] TONDINI F,LANG T,CHEN L,et al.Linking gene expression and oenological traits:Comparison between Torulaspora delbrueckii and Saccharomyces cerevisiae strains[J].International Journal of Food Microbiology,2019,294:42-49.
[31] WHITENER M E B,STANSTRUP J,CARLIN S,et al.Effect of non-Saccharomyces yeasts on the volatile chemical profile of Shiraz wine[J].Australian Journal of Grape and Wine Research,2017,23(2):179-192.
[32] FERREIRA V.Volatile aroma compounds and wine sensory attributes[M]//Managing wine quality.Woodhead,2010:3-28.
[33] RIBÉREAU-GAYON P,DUBOURDIEU D,DONÉCHE B,et al.Handbook of enology,Volume 1:The microbiology of wine and vinifications[M].John Wiley & Sons,2006.
[34] MEDINA K,BOIDO E,FARIÑA L,et al.Increased flavour diversity of Chardonnay wines by spontaneous fermentation and co-fermentation with Hanseniaspora vineae[J].Food Chemistry,2013,141(3):2 513-2 521.
[35] GOBBI M,DE V L,SOLIERI L,et al.Fermentative aptitude of non-Saccharomyces wine yeast for reduction in the ethanol content in wine[J].European Food Research and Technology,2014,239(1):41-48.
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