建立一种简便、高灵敏检测畜禽食用肉中抗病毒药物残留——拉米夫定的共振光散射法。在弱碱性溶液中,刚果红-溴代鲸蜡基吡啶与拉米夫定反应,生成离子缔合物,使共振光散射信号显著增强,并产生具有多个共振光散射峰的新光谱,在398 nm 处出现1 个最大的特征散射峰,在该波长下,拉米夫定的质量浓度在0.006～0.28 mg/L 范围内与缔合物的共振光散射增强强度呈线性关系,检出限为9.5 μg/kg,定量限为0.032 mg/kg。改方法用于生鲜猪肉及鸡肉中拉米夫定的测定,加标回收率为96.5%～103%,相对标准偏差为1.2%～3.5%(n=5)。方法简便、灵敏,可用于畜禽肉中拉米夫定的残留测定。
Asimple and highly sensitive resonance light scattering method for the detection of antiviral drug residue—lamivudine in fresh livestock and poultry meat was established. In a weak alkaline solution, congo red-cetylpyridinium bromide reacted with lamivudine to form an ionic association complex, which significantly enhanced the resonance light scattering signal and produced a new spectrum with multiple resonance light scattering peaks. At the largest characteristic scattering peak which was presented at 398 nm), the mass concentration of lamivudine in the range of 0.006—0.28 mg/L was linearly related to the enhanced intensity of the resonance light scattering of the association complex. The detection limit was 9.5 μg/kg and the quantitative limit was 0.032 mg/kg. The method was used for the determination of lamivudine in fresh pork and chicken, the spike recovery rate was 96.5%-103% and the relative standard deviation was 1.2%-3.5% (n=5). The method was simple and sensitive, and it can be used for the determination of residues lamivudine in livestock and poultry meat.
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