食品与发酵工业 >

2020 , Vol. 46 >Issue 22: 28 - 35

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024343

研究报告

解淀粉芽孢杆菌DMBA-K4高产胞外多糖的发酵条件优化及其抗氧化活性研究

  • 邝嘉华 ,
  • 黄燕燕 ,
  • 胡金双 ,
  • 余佳佳 ,
  • 周钦育 ,
  • 赵珊 ,
  • 刘冬梅
展开
  • (华南理工大学 食品科学与工程学院,广东 广州,510640)
硕士研究生(刘冬梅教授为通讯作者,E-mail:liudm@scut.edu.cn)

收稿日期: 2020-04-29

  修回日期: 2020-05-26

  网络出版日期: 2020-12-11

基金资助

国家自然科学基金(31771908);广州市科技计划项目(201903010015)

收起

Optimization of fermentation conditions and anti-oxidation of exopolysaccharideproduced by Bacillus amyloliquefaciens DMBA-K4

  • KUANG Jiahua ,
  • HUANG Yanyan ,
  • HU Jinshuang ,
  • YU Jiajia ,
  • ZHOU Qinyu ,
  • ZHAO Shan ,
  • LIU Dongmei
Expand
  • (School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)

Received date: 2020-04-29

  Revised date: 2020-05-26

  Online published: 2020-12-11

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摘要

从云南普洱茶中分离出1株产胞外多糖(exopolysaccharide,EPS)的菌株,经16S rDNA鉴定,确定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)DMBA-K4;利用单因素和响应面法优化其胞外多糖的产量,并对其体外抗氧化活性进行研究。经优化后,DMBA-K4产胞外多糖的最佳发酵条件是:4 g/L 蔗糖、5 g/L胰蛋白胨、10 g/L酵母提取粉、5 g/L NaCl、培养时间18 h,pH 7.6,接种量3.10%;在此条件下,其胞外多糖产量为171.31 mg/L,较原始条件提高155.7%。抗氧化实验结果表明,DMBA-K4所产的胞外多糖具有较强的抗氧化活性。当EPS质量浓度为5 mg/mL时,其1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率达78.60%,对·OH的清除率为75.47%。该研究可为微生物胞外多糖的工业生产提供理论支持,丰富新型微生物多糖的应用前景。

关键词： 解淀粉芽孢杆菌; 胞外多糖; 发酵条件; 响应面优化; 抗氧化

本文引用格式

邝嘉华 , 黄燕燕 , 胡金双 , 余佳佳 , 周钦育 , 赵珊 , 刘冬梅 . 解淀粉芽孢杆菌DMBA-K4高产胞外多糖的发酵条件优化及其抗氧化活性研究[J]. 食品与发酵工业, 2020 , 46(22) : 28 -35 . DOI: 10.13995/j.cnki.11-1802/ts.024343

Abstract

An exopolysaccharides (EPS) producing strain was isolated from Yunnan Puer tea.This strain was defined as Bacillus amyloliquefaciens DMBA-K4 by 16S rDNA.Single factor trials and response surface tests were performed to optimize its EPS yield,and its in vitro antioxidant activity was also investigated.The optimal fermentation conditions were as follow:the culture medium consisted of 4 g/L sucrose,5 g/L tryptone,10 g/L yeast,5 g/L NaCl,pH 7.6,inoculator amount of 3.10% and fermented for 18 h.Under these conditions,the maximal yields of EPS increased by 155.7% and reached 171.31mg/L.In vitro antioxidant results showed that EPS had strong antioxidant activity.When the concentration of EPS was 5 mg/mL,the scavenging rate of DPPH free radical and ·OH was 78.60% and 75.47%,respectively.The research provide theoretical support to EPS industrialization and widen the utilization prospect of novel bio-polysaccharide.

Key words： Bacillus amyloliquefaciens; exopolysaccharide; fermentation conditions; response surface; antioxidation

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