食品与发酵工业 >

2020 , Vol. 46 >Issue 22: 91 - 97

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024438

研究报告

利用16S rDNA分析不同地区传统发酵泡菜的细菌多样性

  • 刘巧 ,
  • 罗强 ,
  • 张明 ,
  • 生龙娜雍 ,
  • 关仁梅 ,
  • 罗璠
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  • 1(西南民族大学 生命科学与技术学院,四川 成都,610041);
    2(青藏高原动物遗传资源保护与利用教育部重点实验室(西南民族大学),四川 成都,610041)
硕士研究生(罗璠副教授为通讯作者,E-mail:luofany77@163.com)

收稿日期: 2020-05-18

  修回日期: 2020-06-04

  网络出版日期: 2020-12-11

基金资助

西南民族大学研究生创新型科研项目(CX2018SZ12)

收起

Bacterial diversity analysis of traditional naturally fermented pickles from different regions in China by 16S rDNA

  • LIU Qiao ,
  • LUO Qiang ,
  • ZHANG Ming ,
  • SHENGLONG Nayong ,
  • GUAN Renmei ,
  • LUO Fan
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  • 1(College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China);
    2(Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization,Ministry of Education(Southwest Minzu University),Chengdu 610041,China)

Received date: 2020-05-18

  Revised date: 2020-06-04

  Online published: 2020-12-11

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摘要

为了解不同地区传统发酵泡菜的细菌多样性,采用构建16S rDNA克隆文库的方法,对吉林、河南、辽宁、内蒙古4个地区的传统自然发酵泡菜的细菌群落多样性进行研究。结果表明,4个地区泡菜的细菌多样性、优势度和均匀度存在差异,辽宁地区泡菜中细菌群落的多样性最高,优势属突出,且均匀度较好。而河南地区泡菜的细菌群落多样性,优势度和均匀度最低。经过16S rDNA基因序列分析,实验中的200个克隆子分布于4个属,分别是乳杆菌属(Lactobacillus),肠球菌属(Enterococcus),明串珠菌属(Leuconostoc)以及片球菌属(Pediococcus)。其中L.brevis均为吉林、河南、辽宁、内蒙古地区泡菜的绝对优势菌种。该研究通过探讨不同地区泡菜的细菌多样性,揭示了不同地区泡菜在微生物群落结构上的差异性,为探究泡菜风味物质的形成机理提供理论依据并为开发利用泡菜中的微生物资源奠定基础。

关键词： 泡菜; 细菌多样性; 16SrDNA; 克隆文库; 系统发育分析

本文引用格式

刘巧 , 罗强 , 张明 , 生龙娜雍 , 关仁梅 , 罗璠 . 利用16S rDNA分析不同地区传统发酵泡菜的细菌多样性[J]. 食品与发酵工业, 2020 , 46(22) : 91 -97 . DOI: 10.13995/j.cnki.11-1802/ts.024438

Abstract

In order to understand the bacterial diversity of traditional naturally fermented pickles from different regions,the method of constructing 16S rDNA clone library was used to study the bacterial community diversity of traditional naturally fermented pickles in Jilin,Henan,Liaoning and Inner Mongolia.The results indicated that the pickles of these four regions varied in bacterial diversity,dominance and evenness.The diversity of bacterial community in the pickles in Liaoning was the highest,the dominance was prominent and the uniformity was good.While,the diversity,dominance and evenness were the lowest in Henan pickles.Through 16S rDNA gene sequence analysis,200 clones were found distributing in four genera,namely Lactobacillus,Enterococcus,Leuconostoc and Pediococcus.Lactobacillus brevis was the dominant species of pickles in Jilin,Henan,Liaoning and Inner Mongolia.By exploring the diversity of bacteria in pickles from different regions,this study revealed the differences in the microbial community structure of pickles from different regions,which provides a theoretical basis for the subsequent exploration of the formation mechanism of pickles flavor substances and lays a foundation for the development and utilization of the microbial resources in pickles.

Key words： pickles; bacterial diversity; 16S rDNA; clone library; phylogenetic analysis

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