为开发海鲈鱼鳞,该文以感官评价与质构特性为评价指标,结合单因素实验及正交实验研究确定海鲈鱼鳞制备鱼鳞胨的最佳工艺,同时分析产品的蛋白质含量和微生物指标。结果表明,加入鱼鳞质量1%(质量分数)食用盐和2%(体积分数)白醋复合处理鱼鳞能有效去除海鲈鱼鳞腥味。海鲈鱼鳞与水的质量配比为1:7.5,在常压下经100 ℃水熬煮25 min,过滤后在4 ℃下凝冻2 h,即得到鱼鳞胨成品。产品颜色微白透明,晶莹剔透,口感爽滑,韧性好,无鱼腥味,且呈致密果冻状,不松散,有较好弹性。其蛋白质含量为3.46%,无致病菌,菌落总数1.20×102 CFU/g,符合相关国家卫生标准要求。该产品与果冻等食品比较,其富含蛋白质,且该蛋白质为胶原蛋白。该研究为鱼鳞胨制品的开发提供了技术支撑。
In order to increase the scales value of sea bass,sensory evaluation and texture characteristics was used as evaluation indicators combined with single factor experiment and orthogonal experiment to determine the best process for preparing scales of sea bass.Meanwhile,the protein content and microbial of the product were also been analyzed.The results showed that the optimized conditions was as follows:The combined treatment of fish scales with 1% edible salt and 2% white vinegar;the mass ratio of sea bass fish scales to water was 1:7.5 and boiling at 100 ℃ for 25 minutes under normal pressure.Then the samples were filtered and refrigerated at 4°C for 2 hours to obtain the finished fish scale peptone.Moreover,the color was white and transparent,crystal clear,smooth taste,good toughness,no fishy smell,and dense jelly,not loose,and had good elasticity.Its protein content was 3.46% and the total number of colonies was 1.20×102 CFU/g,which meets the requirements of relevant national health standards.Compared with jelly and other foods,this product was rich in collagen.This research provides technical support for the development of fish scale peptone products.
[1] AYALA M D,LÓPEZ ALBORS O,BLANCO A,et al.Structural and ultrastructural changes on muscle tissue of sea bass,Dicentrarchus labraxL.,after cooking and freezing[J].Aquaculture,2005,250(1):215-31.
[2] 张海燕,吴燕燕,杨少玲,等.聚赖氨酸与魔芋葡甘聚糖复配对海鲈鱼片的保鲜效果[J].食品与发酵工业,2019,45(24):202-208.
[3] CAI L,CAO A,BAI F,et al.Effect of ε-polylysine in combination with alginate coating treatment on physicochemical and microbial characteristics of Japanese sea bass (Lateolabrax japonicas) during refrigerated storage[J].LWT-Food Science and Technology,2015,62(2):1 053-1 059.
[4] 农业部渔业渔政管理局.2019中国渔业统计年鉴[M].北京:中国农业出版社,2019:172.
[5] 顾杨娟,李杰,李富威,等.制备草鱼鱼鳞冻时鱼鳞前处理工艺的优化[J].江苏农业科学,2013,41(2):217-220.
[6] 代昕冉,刘焱,陈力力.淡水鱼鳞综合利用研究进展[J].农产品加工,2018(16):61-66.
[7] HOLÁM,KALVODA J,NOVÁKOVÁH,et al.Possibilities of LA-ICP-MS technique for the spatial elemental analysis of the recent fish scales:Line scan vs.depth profiling[J].Applied Surface Science,2010,257(6):1 932-1 940.
[8] 刘庆慧,刘从力,王采理.鱼鳞营养成分的分析及对高脂饲料大鼠血脂水平的影响[J].中国水产科学,2000(4):56-59.
[9] OGAWA M,PORTIER R J,MOODY M W,et al.Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead seabream (Archosargus probatocephalus)[J].Food Chemistry,2004,88(4):495-501.
[10] PATI F,ADHIKARI B,DHARA S.Isolation and characterization of fish scale collagen of higher thermal stability[J].Bioresource Technology,2010,101(10):3 737-3 742.
[11] 李燕,龚珞军,李文华,等.HACCP计划在鱼鳞冻加工工艺和质量控制中的应用[J].北京水产,2007(1):53-54.
[12] 陈良,李春莲,谌素华,等.鱼鳞胶的提取工艺研究[J].中国水产,2010(1):69-70.
[13] 吴缇,陈舜胜.斑点叉尾鱼骨制取胶原蛋白和骨粉的试验研究[J].南方水产,2009,5(3):36-40.
[14] 曾少葵,刘坤,吴艺堂,等.脱钙罗非鱼鱼鳞明胶提取工艺优化及其理化性质[J].南方水产科学,2013,9(2):38-44.
[15] 沙小梅.鱼鳞明胶的制备及其凝胶强度的研究[D].南昌:南昌大学,2012.
[16] 顾杨娟,李杰,李富威,等.鱼鳞冻热处理工艺优化及性质的研究[J].食品与发酵工业,2013,39(2):102-108.
[17] 朱玲玲,谷绒,董道顺.鱼鳞冻的成冻工艺研究[J].农产品加工(学刊),2013(21):44-45;51.
[18] 顾杨娟.草鱼鱼鳞冻的制备及其性质研究[D].上海:上海海洋大学,2013.
[19] SOMBOONPANYAKUL P,BARBUT S,JANTAWAT P,et al.Textural and sensory quality of poultry meat batter containing malva nut gum,salt and phosphate[J].LWT-Food Science and Technology,2005,40(3):498-505.
[20] ARMENTEROS M,ARISTOY M C,BARAT J M,et al.Biochemical changes in dry-cured loins salted with partial replacements of NaCl by KCl[J].Food Chemistry,2009,117(4):627-33.
[21] 中华人民共和国国家卫生和计划委员会.GB 4789.2—2016 食品微生物学检验-菌落总数测定[J].北京:中华人民共和国国家标准出版社,2016,
[22] 中华人民共和国国家卫生和计划委员会.GB 5009.5—2016 食品安全国家标准-食品中蛋白质的测定[J].北京:中华人民共和国国家标准出版社,2016.
[23] 刘雨萱,陈媛,李美良,等.罗非鱼鱼鳞胶原蛋白的研究进展[J].食品工业科技,2019,40(6):355-360.
[24] 朱晓妮.胶原蛋白在食品中的应用现状及其发展前景分析[J].现代食品,2016(14):11-12.