食品与发酵工业 >

2020 , Vol. 46 >Issue 23: 41 - 46

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024631

研究报告

河豚鱼皮胶原肽螯合锌的组成与急性毒性研究

  • 郭洪辉 ,
  • 陈晖 ,
  • 俞云 ,
  • 方华 ,
  • 陈思谨 ,
  • 洪专
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  • 1(自然资源部第三海洋研究所 海洋生物资源开发利用工程技术创新中心,福建省海洋生物资源开发利用协同创新中心,福建 厦门,361005);
    2(南京中医药大学 药学院,江苏 南京,210023)
博士,副研究员(洪专教授级高级工程师为通讯作者,E-mail:zhong@tio.org.cn)

收稿日期: 2020-06-03

  修回日期: 2020-07-13

  网络出版日期: 2020-12-30

基金资助

福建省科技计划引导性项目(2019N0033);自然资源部第三海洋研究所基本科研业务费专项资金资助项目(海三科2017006、海三科2017026);厦门海洋高技术产业基地创业创新共享服务平台项目(16PFW008SF15);“十三五”北海市海洋经济创新发展示范项目(Bhsfs003)

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Study on the composition and acute toxicity of collagen peptide chelated zinc from puffer skin

  • GUO Honghui ,
  • CHEN Hui ,
  • YU Yun ,
  • FANG Hua ,
  • CHEN Sijin ,
  • HONG Zhuan
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  • 1(Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Xiamen 361005,China);
    2(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

Received date: 2020-06-03

  Revised date: 2020-07-13

  Online published: 2020-12-30

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摘要

对河豚鱼皮胶原肽螯合锌的主要成分、分子质量分布及氨基酸组成作了分析和比较,并通过急性毒性试验,初步评价其毒理学安全性。实验结果表明,河豚鱼皮胶原肽螯合锌的蛋白含量为(82.04±0.68)%,锌含量为(12.86±1.24)%,胶原肽的分子质量主要集中在600 Da附近。氨基酸分析显示,河豚鱼皮胶原肽螯合锌含有丰富的甘氨酸、羟脯氨酸以及大量的天冬氨酸和谷氨酸,基质辅助激光解吸电离飞行时间质谱图显示了肽-锌螯合物的生成。通过急性毒性试验,得出河豚鱼皮胶原肽螯合锌的半数致死量为6.8479 g/kg,95%的可信限为6.122 4～7.676 5 g/kg,根据急性毒性剂量分级标准,属于实际无毒级。

关键词： 河豚鱼皮; 胶原肽螯合锌; 分子质量分布; 氨基酸组成; 急性毒性

本文引用格式

郭洪辉 , 陈晖 , 俞云 , 方华 , 陈思谨 , 洪专 . 河豚鱼皮胶原肽螯合锌的组成与急性毒性研究[J]. 食品与发酵工业, 2020 , 46(23) : 41 -46 . DOI: 10.13995/j.cnki.11-1802/ts.024631

Abstract

In this paper,the main components,molecular weight distribution and amino acid composition of collagen peptide chelated zinc from puffer skin were analyzed and compared.Its toxicological safety was preliminarily evaluated by acute toxicity test.The results showed that the protein content of collagen peptide chelated zinc from pufferfish skin was (82.04±0.68)%,and the zinc content was (12.86±1.24)%.The molecular weight of collagen peptides from puffer skin was mainly concentrated around 600 Da.Amino acid analysis indicated collagen peptide chelated zinc from puffer skin contained rich glycine,hydroxyproline,aspartic acid and glutamate.The MALDI-TOF mass spectrometry showed the generation of the peptide-zinc chelate.Through the acute toxicity test,the LD50 of collagen peptide chelated zinc from puffer skin was 6.847 9 g/kg,and the 95% confidence limit was 6.122 4~7.676 5 g/kg.According to the acute toxicity dose grading standard,the collagen peptide chelated zinc from puffer skin was actually non-toxic.

Key words： puffer skin; collagen peptide chelated zinc; molecular weight distribution; amino acid composition; acute toxicity

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