食品与发酵工业 >

2020 , Vol. 46 >Issue 23: 74 - 80

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024654

研究报告

产阿魏酸酯酶丝状真菌筛选及发酵条件优化

  • 李松 ,
  • 徐朝旭 ,
  • 林陈莹 ,
  • 魏胜华 ,
  • 李艳宾
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  • (安徽工程大学 生物与化学工程学院,安徽 芜湖,241000)
博士,副教授(通讯作者,E-mail:lisong821123@126.com)

收稿日期: 2010-06-05

  修回日期: 2020-07-07

  网络出版日期: 2020-12-30

基金资助

安徽高校协同创新项目(GXXT-2019-034);安徽工程大学中青年拔尖人才培养计划项目(S022019016);生物质资源化高效利用科研创新团队项目(Xjky04201903)

收起

Filamentous fungi with feruloyl esterase ability: Screening and optimization of fermentation conditions

  • LI Song ,
  • XU Zhaoxu ,
  • LIN Chenying ,
  • WEI Shenhua ,
  • LI Yanbin
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  • (School of Biological and Chemical Engineering,Anhui Polytechnic University,Wuhu 241000,China)

Received date: 2010-06-05

  Revised date: 2020-07-07

  Online published: 2020-12-30

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摘要

筛选获得8株产阿魏酸酯酶的丝状真菌,其中1株阿魏酸酯酶产生能力较高,经5.8S-ITS序列比对鉴定为土曲霉。通过单因素试验和响应面试验设计,对该菌株进行了液态发酵培养基优化,得到该菌株产阿魏酸酯酶优化的培养基配方(g/L):葡萄糖30,啤酒糟24.5,豆渣81,FeSO4·7H2O 0.01,KCl 0.5,MgSO4·7H2O 0.5,K2HPO4 1,pH 5.0。利用上述培养基在摇瓶发酵条件下得到的最高酶活力为205 U/L,约为优化前产酶水平的3倍。研究中获得的产阿魏酸酯酶土曲霉菌株及其液态发酵培养条件,为高产阿魏酸酯酶菌株的选育及其后期开发应用奠定了基础。

关键词： 丝状真菌; 土曲霉; 阿魏酸酯酶; 阿魏酸; 响应面设计

本文引用格式

李松 , 徐朝旭 , 林陈莹 , 魏胜华 , 李艳宾 . 产阿魏酸酯酶丝状真菌筛选及发酵条件优化[J]. 食品与发酵工业, 2020 , 46(23) : 74 -80 . DOI: 10.13995/j.cnki.11-1802/ts.024654

Abstract

Eight filamentous fungi with feruloyl esterase ability were screened,and one with the highest yield of feruloyl esterase was identified asAspergillus terreus through sequencing and alignment of 5.8S-ITS.Single factor experiment and response surface methodology were adopted to optimize the liquid fermentation medium ofA.terreus and the optimum medium formula (g/L) was glucose 30,brewer's spent grains 24.5,bean dregs 81,FeSO4·7H2O 0.01,KCl 0.5,MgSO4 7H2O 0.5,K2HPO4 1,with pH 5.0.Under the optimized medium,the highest yield of feruloyl esterase in shaking flask fermentation was 205 U/L,which was about 3 times of the initial yield.The isolated feruloyl esterase producing strain and the optimized liquid fermentation conditions obtained in this study lay a foundation for the breeding of high-yield feruloyl esterase producing strain with an aim for further industrial application.

Key words： filamentous fungi; Aspergillus terreus; feruloyl esterase; ferulic acid; response surface design

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