食品与发酵工业 >

2020 , Vol. 46 >Issue 24: 66 - 73

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024668

研究报告

开阳地区桑葚果园富硒酵母菌的分离、筛选及鉴定

  • 杨新 ,
  • 陈莉 ,
  • 杨双全 ,
  • 卢红梅 ,
  • 章之柱 ,
  • 杨华连 ,
  • 孟洋 ,
  • 魏建敏
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  • 1(贵州大学,贵州省发酵工程与生物制药重点实验室,贵州 贵阳,550025)
    2(贵州大学 酿酒与食品工程学院,贵州 贵阳,550025)
    3(贵州大学 化学与化工学院,贵州 贵阳,550025)
    4(开阳县市场监督管理局,贵州 贵阳,550300)
硕士研究生(陈莉副教授为通讯作者,E-mail:3152539622@qq.com)

收稿日期: 2020-06-07

  修回日期: 2020-09-01

  网络出版日期: 2021-01-13

基金资助

贵州省科技支撑计划项目(黔科合支撑[2019]2317号)

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Isolation, screening and identification of selenium-enriched yeast from mulberry orchard of Kaiyang

  • YANG Xin ,
  • CHEN Li ,
  • YANG Shuangquan ,
  • LU Hongmei ,
  • ZHANG Zhizhu ,
  • YANG Hualian ,
  • MENG Yang ,
  • WEI Jianmin
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  • 1(Guizhou Key Lab of Fermentation Engineering and Biological Pharmacy, Guizhou University, Guiyang 550025, China)
    2(School of Liquor-making and Food Engineering, Guizhou University, Guiyang 550025, China)
    3(School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China)
    4(Kaiyang Administration for Market Regulation, Guiyang, Guizhou 550300, China)

Received date: 2020-06-07

  Revised date: 2020-09-01

  Online published: 2021-01-13

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摘要

为了得到具有富硒能力的酵母菌,从开阳地区富硒桑葚果园中的桑果、桑叶、土壤中分离纯化酵母菌,通过耐硒法和红硒法进行初筛,然后再通过酵母菌对硒的转化率进行复筛,得到1株硒转化率较高的富硒酵母菌SY2,其富硒量为2 278.35 μg/g,生物量为4.31 g/L,硒转化率为49.11%。经WL营养琼脂培养及分子生物学鉴定,鉴定该菌株为酿酒酵母菌(Saccharomyces cerevisiae)。该文对于促进桑葚资源的综合利用,丰富富硒产品的多元化开发,推动开阳县富硒产业的不断发展都具有积极意义。

关键词： 桑葚果园; 富硒酵母菌; 分离筛选; 分子生物学鉴定

本文引用格式

杨新 , 陈莉 , 杨双全 , 卢红梅 , 章之柱 , 杨华连 , 孟洋 , 魏建敏 . 开阳地区桑葚果园富硒酵母菌的分离、筛选及鉴定[J]. 食品与发酵工业, 2020 , 46(24) : 66 -73 . DOI: 10.13995/j.cnki.11-1802/ts.024668

Abstract

In order to obtain yeasts with selenium-enriching ability, we separated and purified some yeasts from mulberry fruit, mulberry leaves and soil in selenium-enriched mulberry orchards in Kaiyang area. Preliminary screening was carried out by selenium-tolerant method and red selenium method, and then selected by the conversion rate of selenium. A selenium-enriched yeast SY2 with high selenium conversion rate was obtained, whose selenium-enriched amount was 2 278.35 μg/g, the biomass was 4.31 g/L, and the selenium conversion rate was 49.11%. After WL nutrient agar culture and molecular biology identification, the strain was identified as Saccharomyces cerevisiae. This will have positive significance for promoting the comprehensive utilization of mulberry resources, diversifying the development of selenium-rich products, and promoting the continuous development of the selenium-rich industry in Kaiyang county.

Key words： mulberry orchard; selenium-rich yeast; separation and screening; molecular biological identification

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