综述与专题评论

植物蛋白泡沫分离技术研究现状

  • 张可可 ,
  • 胡楠 ,
  • 李会珍 ,
  • 张志军
展开
  • (中北大学 化学工程与技术学院,山西 太原,030051)
硕士研究生(张志军教授为通讯作者,E-mail:zjzhang@nuc.edu.cn)

收稿日期: 2020-06-23

  修回日期: 2020-07-16

  网络出版日期: 2021-01-13

基金资助

山西省青年科学基金(201901D211271);山西省科技厅重点研发计划(社会发展)项目(201803D31060);山西省转型综改试验区晋中开发区科技研究项目资助;山西省高等学校科技创新(省立省资助)项目(2020L0270);中北大学2019年校科研基金(XJJ201910)

Advances in foam fractionation technology of plant protein

  • ZHANG Keke ,
  • HU Nan ,
  • LI Huizhen ,
  • ZHANG Zhijun
Expand
  • (College of Chemical Engineering and Technology, North University of China, Taiyuan 030051, China)

Received date: 2020-06-23

  Revised date: 2020-07-16

  Online published: 2021-01-13

摘要

随着“植物蛋白基食品”发展热潮的不断兴起,植物蛋白资源的高效利用成为全球食品行业关注的焦点。目前植物蛋白的分离方法有碱溶酸沉法、色谱法、超滤法、大孔树脂吸附法等,但一定程度上存在着对蛋白破坏大、成本高、能耗大、易污染等缺点。泡沫分离因其能高度富集和回收植物浸提液中的低浓度蛋白,在浓缩回收植物蛋白中展现了较大潜力。该文综述了泡沫分离在浓缩和回收植物纯蛋白和植物蛋白混合体系的研究进展,对于前者而言,重点阐述了操作条件、装置结构和操作方式对纯蛋白分离的影响,以期为植物蛋白的有效分离提供理论参考。

本文引用格式

张可可 , 胡楠 , 李会珍 , 张志军 . 植物蛋白泡沫分离技术研究现状[J]. 食品与发酵工业, 2020 , 46(24) : 278 -283 . DOI: 10.13995/j.cnki.11-1802/ts.024848

Abstract

With the continuous rise of “plant protein-based food” development, the efficient use of plant protein resources has become the focus of global food industry. At present, the separation method of plant protein includes alkali-solution and acid-isolation, chromatography, ultrafiltration, macroporous resin adsorption, etc. However, there are many shortcomings such as the damage of the protein, high cost, high energy consumption, easy pollution and so on. Foam fractionation shows great potential in the industrial separation of plant proteins because it can enrich and recover the low-concentration proteins from their extract liquids. This paper summarized the research progress of concentrating and recovering plant pure protein and plant protein in the mixing system. Moreover, it focused on the effects of operating conditions, device structure and operation modes on the separation of pure protein in order to provide theoretical references for the efficient separation of plant protein.

参考文献

[1] 胡苗苗, 杨海霞, 曹炜, 等.植物蛋白质资源的开发利用[J].食品与发酵工业, 2012, 38(8):137-140.
HU M M, YANG H X, CAO W, et al.The development and current situation of plant protein utilization[J].Food and Fermentation Industries, 2012, 38(8):137-140.
[2] 罗洁霞, 徐克.我国居民家庭膳食蛋白质和脂肪摄入量比较[J].中国食物与营养, 2019, 25(2):79-83.
LUO J X, XU K.Comparative research on protein and fat intake of chinese residents[J].Food and Nutrition in China, 2019, 25(2):79-83.
[3] 张英蕾, 姚鑫淼, 卢淑雯, 等.碱溶酸沉法提取黑豆蛋白工艺优化[J].中国食品添加剂, 2019, 30(1):60-68.
ZHANG Y L, YAO X M, LU S W, et al.Optimization of black soybean protein extraction process by alkali and acid precipitation[J].China Food Additives, 2019, 30(1):60-68.
[4] 安家静, 卢红梅, 陈莉, 等.碱提酸沉法提取薏仁碎米中蛋白质的研究[J].中国酿造, 2018,37(6):97-102.
AN J J, LU H M, CHEN L, et al.Coix seed protein extraction using alkali extraction and acid precipitation[J].China Brewing, 2018,37(6):97-102.
[5] 陈雪娇, 梁兵, 宁志高, 等.棉籽蛋白质提取工艺的研究[J].沈阳化工大学学报, 2019, 33(1):34-38.
CHEN X J, LIANG B, NING Z G, et al.Study on extraction technology of cottonseed protein[J].Journal of Shenyang University of Chemical Technology, 2019, 33(1):34-38.
[6] 刘凯, 梁俊玉, 肖引, 等.磷酸盐法提取杏仁蛋白的工艺研究[J].武警医学, 2013, 24(9):783-785.
LIU K, LIANG J Y, XIAO Y, et al.Extracting process conditions from almond dregs by phosphate method[J].Medical Journal of the Chinese People's Armed Police Force, 2013, 24(9):783-785.
[7] 李玉珍, 肖怀秋.碎籼米蛋白质中性蛋白酶酶法提取工艺优化[J].粮油食品科技, 2017, 25(2):22-27.
LI Y Z, XIAO H Q.Optimization of enzymatic extraction of protein from broken indica rice[J].Science and Technology of Cereals, Oils and Foods, 2017, 25(2):22-27.
[8] 江玉琴, 柯伟倩, 邓放明.碱性蛋白酶提取湘莲蛋白质的工艺优化[J].粮食科技与经济, 2018, 43(2):108-110;115.
JIANG Y Q, KE W Q, DENG F M.Optimization of the extraction process of Xianglian protein by alkaline protease[J].Grain Science and Technology and Economy, 2018, 43(2):108-110;115.
[9] 翁德会, 杨文婷, 汤雅萍, 等.厚朴中蛋白质提取方法的优化[J].食品研究与开发, 2018, 39(18):66-69.
WENG D H, YANG W T, TANG Y P, et al.Optimization of the extraction method of protein in magnolia officinalis[J].Food Research and Development, 2018, 39(18):66-69.
[10] 侯兆乾, 刘鑫阳, 史超, 等.冻融法和超声破碎法提取螺旋藻中藻蓝蛋白的工艺研究[J].内蒙古农业大学学报(自然科学版), 2017, 38(2):69-75.
HOU Z Q, LIU X Y, SHI C, et al.The research of extraction procedure by using method of freezing-thawing and ultrasonic broken process to extract phycocyanin from spirulina algae[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2017, 38(2):69-75.
[11] 金周雨, 丁淼, 康骁, 等.海鲜菇蛋白质提取条件的优化[J].现代食品, 2018(22):124-128.
JIN Z Y, DING M, KANG X, et al.Optimization of extraction conditions of protein from hypsizygus marmoreus[J].Modern Food, 2018(22):124-128.
[12] 王维坚, 潘艳.绿豆蛋白热水浸提工艺优化[J].食品与机械, 2013, 29(5):154-157.
WANG W J, PAN Y.Optimization on extracting conditions for mung bean protein with response surface methodology[J].Food & Machinery, 2013, 29(5):154-157.
[13] 刘俊红, 袁冰倩, 李爽, 等.超声波-碱法提取小麦麸皮蛋白质的工艺研究[J].食品工业, 2018, 39(9):5-8.
LIU J H, YUAN B Q, LI S, et al.Protein extraction from wheat bran by ultrasound and alkali[J].The Food Industry, 2018, 39(9):5-8.
[14] 邵婷, 刘昕, 黎重阳, 等.超声波辅助高效提取辣木籽中的蛋白质[J].食品与发酵工业, 2019, 45(9):207-213.
SHAO T, LIU X, LI C Y, et al.Ultrasonic-assisted highly efficient extraction of proteins from Moringa oleifera seeds[J].Food and Fermentation Industries, 2019, 45(9):207-213.
[15] 刘龙, 张炜, 陈元涛, 等.菠菜叶蛋白泡沫法分离工艺的优化[J].食品与机械, 2017, 33(6):169-175.
LIU L, ZHANG W, CHEN Y T, et al.Optimization on foam separation process for spinach leaf protein[J].Food & Machinery, 2017, 33(6):169-175.
[16] LI R, FU N, WU Z L, et al.Enhancing protein self-association at the gas-liquid interface for foam fractionation of bovine serum albumin from its highly diluted solution[J].Chemical Engineering Research and Design, 2016, 109:638-646.
[17] 董爱军, 杨鑫, 张英春, 等.高效液相色谱法分离测定牦牛乳中的8种蛋白质[J].分析测试学报, 2012, 31(8):972-976.
DONG A J, YANG X, ZHANG Y C, et al.Separation and quantification of major protein in yak milk by high performance liquid chromatographic method[J].Journal of Instrumental Analysis, 2012, 31(8):972-976.
[18] 李敏婧, 杨洁, 杨迎春, 等.利用反相高效液相法测定马乳中的乳清蛋白[J].食品与发酵工业, 2017, 43(9):209-214.
LI M J, YANG J, YANG Y C, et al.Determination of whey proteins in mare milk by reversed-phase high-performance liquid chromatography[J].Food and Fermentation Industries, 2017, 43(9):209-214.
[19] 顾文芬, 曾凡逵, 程锦春.超滤法从马铃薯淀粉加工分离汁水中回收蛋白质的研究[J].现代食品科技, 2018, 34(3):131-136.
GU W F, ZENG F K, CHENG J C.Study on the protein recovery from potato starch processing water by ultrafiltration[J].Modern Food Science and Technology, 2018, 34(3):131-136.
[20] 秦冬玲, 马玉洁, 陆国太, 等.大豆乳清蛋白的超滤分离及膜污染分析[J].食品工业科技, 2016, 37(23):72-76.
QIN D L, MA Y J, LU G T, et al.Ultrafiltration and membrane fouling analysis for the separation of soybean whey protein[J].Science and Technology of Food Industry, 2016, 37(23):72-76.
[21] 古静燕, 李新胜, 马超, 等.大孔吸附树脂去除金针菇粗多糖中蛋白的研究[J].食品工业, 2016, 37(3):51-53.
GU J Y, LI X S, MA C, et al.Deproteinization from flammulina velutipes polysaccharide extraction by macroporous adsorption resin[J].The Food Industry, 2016, 37(3):51-53.
[22] 秦俊哲, 张慧洋.大孔吸附树脂去除桑黄粗多糖中蛋白的研究[J].食品工业科技, 2010, 31(12):263-265.
QIN J Z, ZHNAG H Y.Deproteinization from Phellinus linteus crude polysaccharide by macroporous adsorption resin[J].Science and Technology of Food Industry, 2010, 31(12):263-265.
[23] 朱海兰. 温度和气体分布器孔径对泡沫分离过程的影响及气体利用方式的初步研究[D].天津:河北工业大学, 2013.
ZHU H L.Effects of temperature and gas distributor pore on foam separation and the preliminary study of gas utilization[D].Tianjin:Hebei University of Technology, 2013.
[24] 赵艳丽, 张芳, 吴兆亮, 等.不同pH下离子强度对泡沫分离乳清蛋白的影响[J].河北工业大学学报, 2012, 41(4):40-45.
ZHAO Y L, ZHANG F, WU Z L, et al.Effect of ionic strength on the foam separation of whey protein with different pH[J].Journal of Hebei University of Technology, 2012, 41(4):40-45.
[25] 路帅, 孙培冬, 季晓彤, 等.杏仁蛋白的两级泡沫分离工艺优化[J].食品工业科技, 2018, 39(12):200-204.
LU S, SUN P D, JI X T, et al.Optimization of two-stage foam separation of almond protein[J].Science and Technology of Food Industry, 2018, 39(12):200-204.
[26] 孙红秀. 泡沫分离蛋白质研究[D].天津:天津大学, 2013.
SUN H X.Study on foam fractionation of proteins[D].Tianjin:Tianjin University, 2013.
[27] LI R, WU Z L, WANG Y J, et al.Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin[J].Biotechnology Reports, 2016, 9:46-52.
[28] 李轩领. 亚麻籽胶酸性多糖和亚麻籽蛋白的泡沫分离研究[D].西宁:青海师范大学, 2015.
LI X L.Research of acidic polysaccharides in flaxseed gum and flaxseed protein by foam separation[D].Xining:Qinghai Normal University, 2015.
[29] 申秋华, 吴兆亮, 殷昊, 等.液相压力对泡沫分离过程中气泡大小及分离特性的影响[J].河北工业大学学报, 2010, 39(6):62-65;71.
SHEN Q H, WU Z L, YIN H, et al.Effects of pressure on bubble size and separation of bovine serum albumin in a bubble column[J].Journal of Hebei University of Technology, 2010, 39(6):62-65;71.
[30] 朱海兰, 吴兆亮, 胡滨.温度对泡沫分离过程影响的研究[J].河北工业大学学报, 2013, 42(2):55-60.
ZHU H L, WU Z L, HU B.The effect of temperature on foam separation process[J].Journal of Hebei University of Technology, 2013, 42(2):55-60.
[31] 胡滨, 朱海兰, 吴兆亮.气体分布器孔径对泡沫分离过程影响的研究[J].高校化学工程学报, 2014, 28(2):246-251.
HU B, ZHU H L, WU Z L.The Effect of the pore size of gas distributor on foam separation process[J].Journal of Chemical Engineering of Chinese Universities, 2014, 28(2):246-251.
[32] 刘海彬. 桑叶、紫花苜蓿叶蛋白的泡沫分离行为研究[D].西宁:青海师范大学, 2016.
LIU H B.Study on the separation behavior of Medicago sativa、Mulberry of leaf protein with foam method[D].Xining:Qinghai Normal University, 2016.
[33] 刘颖. 泡沫分离法制备甘薯蛋白的工艺及其特性研究[D].福州:福建农林大学, 2013.
LIU Y.The technology of sweet potato protein separation using foam fractionation and the research on it′s properties[D].Fuzhou:Fujian Agriculture and Forestry University, 2013.
[34] 刘龙, 张炜, 陈元涛, 等.菠菜叶蛋白泡沫法分离工艺的优化[J].食品与机械, 2017, 33(6):169-175.
LIU L, ZHANG W, CHEN Y T, et al.Optimization on foam separation process for spinach leaf protein[J].Food & Machinery, 2017, 33(6):169-175.
[35] 张哲, 吴兆亮, 龙延, 等.垂直筛板构件强化SDS在泡沫分离液相吸附的研究[J].高校化学工程学报, 2015, 29(3):538-543.
ZHANG Z, WU Z L, LONG Y, et al.Enhancement of interfacial adsorption of sds in foam separation columns with vertical sieve tray internal[J].Journal of Chemical Engineering of Chinese Universities, 2015, 29(3):538-543.
[36] 王连杰. 液相折流板强化泡沫分离中吸附性能的研究[D].天津:河北工业大学, 2014.
WANG L J.Enhancing the adsorption in the bulk liquid by using foam separation column fitted with baffles[D].Tianjin:Hebei University of Technology, 2014.
[37] 孙景辉. 泡沫相分离塔分离蛋白质的工艺标准研究[J].中国石油和化工标准与质量, 2016, 36(9):117-118.
SUN J H.Study on the technology standard of protein separation in foam phase column[J].China Petroleum and Chemical Standard and Quality, 2016, 36(9):117-118.
[38] 李瑞. 螺旋内构件强化泡沫分离过程作用机制研究[D].天津:河北工业大学, 2012.
LI R.Study on mechanisms of the spiral interal component in process intensification of foam fractionation[D].Tianjin:Hebei University of Technology, 2012.
[39] 杨全文, 吴兆亮, 殷昊, 等.螺旋构件强化排液的泡沫分离塔用于牛血清蛋白分离[J].化工学报, 2011, 62(4):1 000-1 005.
YANG Q W, WU Z L, YIN H, et al.Enhancing foam drainage using foam fractionation column with spiral internals for separation of bovine serum albumin[J].CIESC Journal, 2011, 62(4):1 000-1 005.
[40] 吴兆亮, 钱少瑜, 郑辉杰, 等.一种泡沫分离过程中用于增强泡沫排液的设备[J].科学通报, 2010, 55(10):950-956.
WU Z L, QIAN S Y, ZHENG H J, et al.A device used to enhance foam discharge during foam separation[J].Chinese Science Bulletin, 2010, 55(10):950-956.
[41] 卢珂, 吴兆亮, 侯凯湖, 等.泡沫相塔壁对泡沫分离牛血清蛋白分离性能的影响[J].化工学报, 2012, 63(6):1 765-1 772.
LU K, WU Z L, HOU K H, et al.Effect of column wall with foam phase on foam separation performances for bovine serum albumin[J].CIESC Journal, 2012, 63(6):1 765-1 772.
[42] 孙瑞娉, 殷昊, 卢珂, 等.两级泡沫分离废水中大豆蛋白的工艺[J].农业工程学报, 2010, 26(11):374-378.
SUN R C, YIN H, LU K, et al.Technology of soy protein separation from wastewater by two-stage foam fractionation[J].Transactions of the Chinese Society of Agricultural Engineering, 2010, 26(11):374-378.
[43] JIANG C S, WU Z L, LI R, et al.Technology of protein separation from whey wastewater by two-stage foam separation[J].Biochemical Engineering Journal, 2011, 55(1):43-48.
[44] LIU Z M, WU Z L, LI R, et al.Two-stage foam separation technology for recovering potato protein from potato processing wastewater using the column with the spiral internal component[J].Journal of Food Engineering, 2013, 114(2):192-198.
[45] LI R, WU Z L, WANG Y J, et al.Pilot study of recovery of whey soy proteins from soy whey wastewater using batch foam fractionation[J].Journal of Food Engineering, 2014, 142:201-209.
[46] 陈晨, 陈复生, 刘伯业.蛋白质-多糖复合物研究进展[J].食品工业, 2019, 40(2):225-229.
CHEN C, CHEN F S, LIU B Y.Research advances on protein-polysaccharide compounds[J].The Food Industry, 2019, 40(2):225-229.
[47] 谭相伟, 吴兆亮, 贾永生, 等.泡沫分离技术在蛋白质多元体系分离中的应用[J].化工进展, 2005(5):510-513.
TAN X W, WU Z L, JIA Y S, et al.Application of foam separation in multicomponent mixture of proteins[J].Chemical Industry and Engineering Progress, 2005(5):510-513.
[48] 寇倩云, 吴兆亮, 胡楠.牛血清白蛋白和溶菌酶混合溶液的泡沫性能与其浓度的关系[J].化工学报, 2015, 66(10):4 107-4 114.
KOU Q Y, WU Z L, HU N.Relationship between foam properties and protein concentration in aqueous solution of bovine serum albumin and lysozyme[J].CIESC Journal, 2015, 66(10):4 107-4 114.
[49] SUZUKI AKIRA, YASUHARA KAZUKI, SEKI HIDESHI, et al.Selective foam separation of binary protein solution by sds complexation method[J].J Colloid Interface Sci, 2002, 253(2):402-408.
[50] TADASHI NAKABAYASHI, YOICHI TAKAKUSAGI, KAZUKI IWABATA, et al.Foam fractionation of protein:Correlation of protein adsorption onto bubbles with a pH-induced conformational transition[J].Anal Biochemistry, 2011, 419(2):173-179.
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