食品与发酵工业 >

2021 , Vol. 47 >Issue 1: 8 - 13

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025066

研究报告

高表达MAL62基因对面包酵母耐高糖的影响

  • 孙溪 ,
  • 刘海晴 ,
  • 张军 ,
  • 范志华 ,
  • 黄亮
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  • 1(天津农学院 食品与生物工程学院,天津, 300384)
    2(天津市农副产品深加工技术工程中心,天津, 300384)
    3(天津农学院 农学与资源环境学院,天津, 300384)
博士,讲师(通讯作者,E-mail:sunxi@tjau.edu.cn)

收稿日期: 2020-07-15

  修回日期: 2020-08-12

  网络出版日期: 2021-02-03

基金资助

国家自然科学基金项目(31701569); 天津市青年拔尖人才项目(TJTZJH-QNBJRC-1-19)

收起

Overexpression of MAL62 increased sugar tolerance of baker’s yeast

  • SUN Xi ,
  • LIU Haiqing ,
  • ZHANG Jun ,
  • FAN Zhihua ,
  • HUANG Liang
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  • 1(College of Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China)
    2(Tianjin Engineering Research Center of Agricultural Products Processing, Tianjin 300384, China)
    3(College of Agronomy and Resource Environment, Tianjin Agricultural University, Tianjin 300384, China)

Received date: 2020-07-15

  Revised date: 2020-08-12

  Online published: 2021-02-03

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摘要

面包酵母(Saccharomyces cerevisiae)的抗逆性对于烘焙工业至关重要。以前期获得的耐冷冻酵母突变株B+MAL62为研究对象,测定其在高糖环境下的生长特性、形态特征、胞内海藻糖与甘油的积累以及产气的变化,并与市售高糖酵母进行对比。研究发现在质量分数为40%～60%的糖胁迫环境下,B+MAL62菌株的胞内海藻糖与甘油水平分别比对照菌株提升55.03%～64.27%与1.2～1.3倍,且高糖环境下B+MAL62具有更好的细胞形态稳定性,其产气速度以及最终产气量可优于市售高糖酵母。结果表明,麦芽糖酶编码基因MAL62高表达可增强面包酵母耐高糖能力。

关键词： 面包酵母; 海藻糖; 甘油; 麦芽糖酶; 产气

本文引用格式

孙溪 , 刘海晴 , 张军 , 范志华 , 黄亮 . 高表达MAL62基因对面包酵母耐高糖的影响[J]. 食品与发酵工业, 2021 , 47(1) : 8 -13 . DOI: 10.13995/j.cnki.11-1802/ts.025066

Abstract

Stress tolerance of baker’s yeast (Saccharomyces cerevisiae) is critical to the baking industry. In this study, the high sugar tolerance of cryotolerant yeast B+MAL62 obtained previously was determined and its growth characteristics, morphological stability, intracellular trehalose and glycerol content and gas production under high sugar stress were tested and compared with the commercially high-sugar tolerant yeast. Results showed that compared with the control strain, at 40%-60% sugar stress, the intracellular trehalose and glycerol levels in B+MAL62 strain were increased by 55.03%-64.27% and 1.2-1.3 times, respectively. Moreover, its morphological stability, gas production rate and final gas production in high sugar stress were better than the control strain and the commercial sugar-tolerance yeast. These results showed that overexpression of MAL62 could improve the sugar tolerance of baker's yeast. The study reveals a new way of enhancing multi-tolerance of baker’s yeast and improving the knowledge of how the maltose metabolism affects the multi-tolerance of baker’s yeast. This study provides a valuable insight into breeding of novel multi-resistant baker's yeast strains and a better understanding of other sugar metabolism, which has the potential to promote the technological level of yeast industry.

Key words： baker’s yeast; trehalose; glycerol; maltase; gas production

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