食品与发酵工业 >

2021 , Vol. 47 >Issue 1: 35 - 42

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025181

研究报告

新疆哈萨克族传统风干肉中真菌多样性分析

  • 王俊钢 ,
  • 李宇辉 ,
  • 刘成江 ,
  • 郭安民 ,
  • 岳建平
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  • 1(新疆农垦科学院 农产品加工研究所,新疆 石河子,832000)
    2(农产品加工重点实验室(新疆农垦科学院),新疆 石河子,832000)
    3(额敏县新大同创生物工程有限责任公司,新疆 额敏,834699)
硕士,副研究员(李宇辉副研究员为通讯作者,E-mail:liyuhui615@sina.com)

收稿日期: 2020-07-24

  修回日期: 2020-08-24

  网络出版日期: 2021-02-03

基金资助

国家自然基金项目(31860437);新疆生产建设兵团科技创新人才计划项目(2020CB024)

收起

Diversity of fungus in traditional air-dried meat of Xinjiang Kazakh

  • WANG Jungang ,
  • LI Yuhui ,
  • LIU Chengjiang ,
  • GUO Anmin ,
  • YUE Janping
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  • 1(Institute of Agro-products Processing Science and Technology,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,China)
    2(Key Laboratory of Agro-Products Processing,XinJiang Academy of Agricultural And Reclamation Science,Shihezi 832000,China)
    3(Emin County Xinda Tongchuang Biological Engineering limited liability company,Emin 834699,China)

Received date: 2020-07-24

  Revised date: 2020-08-24

  Online published: 2021-02-03

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摘要

为明确新疆哈萨克族传统风干肉中真菌结构组成,采用IlluminaMiSeq高通量测序技术对新疆传统风干肉样品中真菌内转录间隔区(internal transcribed spacer, ITS1)基因序列进行分析,比较新疆北疆地区不同来源风干肉中真菌群落结构组成,并对其进行差异性分析。结果表明,4个组26份样品共获得了1 936 392对有效的基因序列条带,371个运算分类单位(operational taxonomic unit,OTU),其中4组共有的OTU 数目为84个,26份样品中共有的OTU数目仅为13个。真菌属水平检测结果表明,假丝酵母属(Candida)30.09％、Mrakia 16.26％、德巴利酵母属(Debaryomyces)11.14％、球腔菌属(Mycosphaere)6.37％、青霉属(Penicillium)4.23％、曲霉属(Aspergillus)2.39％、Mrakiella 2.88％、Naganishia 1.71％和维希尼克氏酵母(Vishniacozyma)1.55％为优势菌群。新疆传统风干肉中真菌菌群具有丰富的多样性,且大部分菌群对肉的发酵起到积极作用,高通量测序技术能够很好地对传统风干肉中真菌多样性监测。

关键词： 传统风干肉; 真菌多样性; 高通量测序

本文引用格式

王俊钢 , 李宇辉 , 刘成江 , 郭安民 , 岳建平 . 新疆哈萨克族传统风干肉中真菌多样性分析[J]. 食品与发酵工业, 2021 , 47(1) : 35 -42 . DOI: 10.13995/j.cnki.11-1802/ts.025181

Abstract

The aim of this study was to elucidate the structure of fungi in traditional dried meat of Kazak nationality in Xinjiang,the gene sequences of internal transcribed spacer 1 (ITS1) of fungi in traditional dried meat samples of Xinjiang were analyzed by IlluminaMiSeq high-throughput sequencing technology.The results showed that a total of 1 936 392 gene sequence bands were obtained from 26 samples in four groups,and 371 operational taxonomic unit (OTU) numbers were obtained.The total number of OTU in four groups was 84,and the total number of OTU in 26 samples was only 13.The dominant fungi at genus level were Candida 30.09%,Mrakia 16.26%,Debaryomyces 11.14%,Mycosphaere 6.37%,Penicillium 4.23%,Aspergillus 2.39%,Mrakiella 2.88%,Naganishia 1.71% and Vishniacozyma 1.55%.At the level of phylum classification,the structure of fungal flora in air-dried meat was similar to that of soil,air,and water.At the genus level,Candida,Debaryomyces and Aspergillus had developed into dominant bacteria.These fungi could produce proteases and lipases,which are critical to the quality of meat products during fermentation.The fungal flora in Xinjiang traditional air-dried meat is rich in diversity,and most of them play a positive role in meat fermentation.High-throughput sequencing technology can monitor the fungal diversity in traditional air-dried meat.

Key words： traditional dried meat; fungusdiversity; high-throughput sequencing

参考文献

[1] 刘娅,唐慧.浅析新疆传统食品的工业化[J].食品科技,2007,28(10):18-21.
LIU Y, TANG H.Industriaiation of Xinjiang traditiona1 food[J].Food Science and Technology,2007,28(10):18-21.
[2] 竺尚武,杨耀寰,王锡渊,等.金华火腿口味及呈味物质的研究[J].食品科学,1993,14(3):8-11.
ZHU S W,YANG Y H,WANG X Y,et al.Investigation on tastes and taste compounds of jin-huaham[J].Food Science,1993,14(3):8-11.
[3] KABAN G.Changes in the composition of volatile compounds and in microbiological and physicochemical parameters during pastrma processing[J].Meat science,2009,82(1):17-23.
[4] PALEARI M A,BERETTA G,COLOMBO F,et al.Buffalo meat as a salted and cured product[J].Meat Science,2000,54(4):365-367.
[5] 王俊钢,李宇辉,郭安民,等.新疆风干牛肉成熟过程中理化及微生物特性分析[J].食品与发酵工业,2016,42(10):129-133.
WANG J G,LI Y H,GUO A M,et al.Physico-chemical and microbial properties of Xinjiang dry-cured beef during the ripening process[J].Food and Fermentation Industries,2016,42(10):129-133.
[6] GAO P,WANG W,JIANG Q,et al.Effect of autochthonous starter cultures on the volatile flavour compounds of Chinese traditional fermented fish (Suan yu)[J].International Journal of Food Science and Technology,2016,51(7):1 630-1 637.
[7] GAO P,WANG W,XIA W,et al.Lipolysis and lipid oxidation caused by Staphylococcus xylosus 135 and Saccharomyces cerevisiae 31 isolated from Suan yu,a traditional Chinese low-salt fermented fish[J].International Journal of Food Science and Technology,2016,51(2):419-426.
[8] 陈东华,范东翠.发酵肉制品风味的研究进展[J].农产品加工,2008(3):63-65.
CHEN D H,FAN D C.Research progress on flavor of fermented meat products[J].Journal of Agricultural Products Processing,2008(3):63-65.
[9] 鄯晋晓,盛占武.发酵肉制品中微生物的作用[J].肉类工业,2007(2):15-18.
SHAN J X,SHENG Z W.The function of microorganisms in fermented meat products[J].Meat Research,2007(2):15-18.
[10] FLORES M,DUR M A,MARCO A,et al.Effect of Debaryomyces spp.on aroma formation and sensory quality of dry-fermented sausages[J].Meat Science,2004,68(3):439-46.
[11] SIRINE,BEN,SLIMA,et al.Effect of partial replacement of nitrite with a novel probiotic Lactobacillus plantarum TN8 on color,physico-chemical,texture and microbiological properties of beef sausages[J].LWT-Food Science and Technology,2017,86:219-226.
[12] NAM Y D,LEE S Y,LIM S I.Microbial community analysis of Korean soybean pastes by next-generation sequencing[J].International Journal of Food Microbiology,2012,155(1-2):36-42.
[13] NAM Y D,PARK S L,LIM S I.Microbial composition of the korean traditional food “kochujang” analyzed by a massive sequencing technique[J].Journal of Food Science,2012,77(4):250-256.
[14] NAM Y D,YI S H,LIM S I.Bacterial diversity of cheonggukjang,a traditional Korean fermented food,analyzed by barcoded pyrosequencing[J].Food Control,2012,28(1):135-142.
[15] HARCOMBE W R,RIEHL W J,DUKOVSKI I,et al.Metabolic resource allocation in individual microbes determines ecosystem interactions and spatial dynamics[J].Cell Reports,2014,7(4):1 104-1 115.
[16] GILLES K,BERNARD T,GEORGES D,et al.Metagenomic insights into the dynamics of microbial communities in food[J].International Journal of Food Microbiology,2015,213:31-39.
[17] QUIJADA N M,FILIPPIS F D,JAVIER S,et al.Different Lactobacillus populations dominate in “Chorizo de León” manufacturing performed in different production plants[J].Food Microbiology,2018,70:94-102.
[18] REBECCHI A,PISACANE V,MIRAGOLI F,et al.High-throughput assessment of bacterial ecology in hog,cow and ovine casings used in sausages production[J].International Journal of Food Microbiology,2015,212(6):49-59.
[19] 田建军,张开屏,杨明阳,等.应用Illumina MiSeq测序技术比较风干肉中细菌多样性和微生物安全性[J].食品科学,2019,40(8):33-40.
TIAN J J,ZHANG K P,YANG M Y,et al.Comparative bacterial diversity analysis and microbial safety assessment of air-dried meat products by Illumina MiSeq sequencing technology[J].Food science,2019,40(8):33-40.
[20] TUOMISTO H.A diversity of beta diversities:Straightening up a concept gone awry.Part 1.Defining beta diversity as a function of alpha and gamma diversity[J].Ecography,2010,33(1):2-22.
[21] TANJA M,SALZBERG S L.FLASH:Fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2 957-2 963.
[22] EDGAR R.C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nature Methods,2013,10(10):996-1 006.
[23] OBERAUNER L,ZACHOW C,LACKNER S,et al.The ignored diversity:Complex bacterial communities in intensive care units revealed by 16S pyrosequencing[J].Sci Rep,2013,3:1 413-1 425.
[24] BALDAUF S L.Phylogeny for the faint of heart:A tutorial[J].Trends in Genetics,2003,19(6):345-351.
[25] CORE R,R R D C T,TEAM R,et al.A language and environment for statistical computing[J].Computing,2015,1:12-21.
[26] XU J,LIAN F,ZHAO L,et al.Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula[J].Isme Journal,2014,9(3):552-562.
[27] QIU Z,LI N,LU X,et al.Characterization of microbial community structure and metabolic potential using Illumina MiSeq platform during the black garlic processing[J].Food Research International,2018,106:428-438.
[28] ZHANG M,ZHANG M,ZHANG C,et al.Pattern extraction of structural responses of gut microbiota to rotavirus infection via multivariate statistical analysis of clone library data[J].FEMS Microbiology Ecology,2009,70(2):21-6.
[29] 高庆超,常应九,马蓉,等.黑果枸杞酵素自然发酵过程中微生物群落的动态变化[J].食品与发酵工业,2019,45(13):126-133.
GAO Q C,CHANG Y J,MA R,et al.Dynamic changes in microbial community during natural fermentation of Lycium Ruthenicum Murr.enzymes[J].Food and Fermentation Industries,2019,45(13):126-133.
[30] 陈新.季节性雪被下新疆天山雪岭云杉林凋落物分解与土壤微生物的关系[D].乌鲁木齐:新疆大学,2019.
CHEN X.Relationship between litter decomposition and soil microbes of Schrenk sprucein forest under seasonal snowfall in Tianshan mountains,Xinjiang[D].Urumqi:Xinjiang University,2019.
[31] BOLUMAR T,SANZ Y,ARISTOY M C,et al.Protease B from Debaryomyces hansenii:Purification and biochemical properties[J].International Journal of Food Microbiology,2005,98(2):167-177.
[32] DURA M A,FLORES M,TOLDRA F.Effect of growth phase and dry-cured sausage processing conditions on Debaryomyces spp.generation of volatile compounds from branched-chain amino acids[J].Food Chemistry,2004,86(3):391-399.
[33] 赵永强,王安凤,陈胜军,等.米曲霉和鲁氏酵母协同发酵优化合浦珠母贝肉酶解液风味[J].食品与发酵工业,2019,45(15):115-120.
ZHAO Y Q,WANG A F,CHEN S J,et al.Synergistic fermentation of Aspergillus oryzae and Saccharomyces rouxii to optimize enzymatic hydrolysate flavor of Pinctada fucata meat[J].Food and fermentation industries,2019,45(15):115-120.
[34] 文开勇,汪月,文鹏程,等.四川传统腊肉中微生物群落结构研究[J].食品与发酵工业,2020,46(3):36-42.
WEN K Y,WANG Y,WEN P C,et al.Study on microbial community structure in Sichuan traditional bacon[J].Food and fermentation industries,2020,46(3):36-42.
[35] CORRAL S, BELLOCH C, LÓPEZ-DíEZ,et al.Lipolysis and aroma generation as mechanisms involved in masking boar taint in sodium reduced fermented sausages inoculated with Debaryomyces hansenii yeast[J].Journal of the Science of Food and Agriculture,2018,98(4):2 121-2 130.
[36] 韦依侬,张涛,李苗苗,等.即食鱼糜制品贮藏过程中的品质稳定性研究[J].食品工业科技,2017,38(16):285-290.
WEI N N,ZHANG T,LI M M,et al.Study on quality stability of ready-to-eat surimi products during storage process[J].Science and Technology of Food Industry,2017,38(16):285-290.
[37] 史笑娜,黄峰,张良,等.红烧肉加工过程中脂肪降解氧化和挥发性风味物质的变化研究[J].现代食品科技,2017,33(3):257-265.
SHI X N,HUANG F,ZHANG L,et al.Changes in lipolysis,lipid oxidation,and volatile flavor compounds during the processing of red braised pork[J].Modern Food Science and Technology,2017,33(3):257-265.
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