食品与发酵工业 >

2021 , Vol. 47 >Issue 1: 138 - 142

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024873

研究报告

山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究

  • 高雪婧 ,
  • 罗思奕 ,
  • 汤凯洁 ,
  • 罗秋水
展开
  • (江西农业大学 食品学院,江西 南昌,330045)
硕士研究生(罗秋水实验师为通讯作者,E-mail:lqsh2001@163.com)

收稿日期: 2020-06-28

  修回日期: 2020-07-23

  网络出版日期: 2021-02-03

基金资助

国家自然科学基金项目(31760488);江西农业大学大学生创新创业训练项目(202010410231)

收起

Effect of crude hawthorn glycoprotein on hypolipidemic and antioxidant activity in high-fat mice

  • GAO Xuejing ,
  • LUO Siyi ,
  • TANG Kaijie ,
  • LUO Qiushui
Expand
  • (School of Food Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China)

Received date: 2020-06-28

  Revised date: 2020-07-23

  Online published: 2021-02-03

Fold

摘要

采用水提醇沉法提取有效成分,Sevage法去除游离蛋白,DEAE-52 纤维素层析及SephadexG-75纯化制备山楂粗糖蛋白,并选择昆明(KM)小鼠为研究对象,随机分为空白对照组、高脂模型组、山楂粗糖蛋白低、中、高剂量组。8周后检测小鼠血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量;检测肝脏过氧化氢酶(catalase,CAT)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性,总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malonaldehyde,MDA)含量。结果表明,与空白对照组相比,高脂模型组的TC、TG、MDA水平升高,HDL-C、CAT、T-SOD、T-AOC降低,且均极显著差异(P<0.01);与高脂模型组相比,山楂粗糖蛋白组的TC、TG、MDA水平降低(P<0.05),HDL-C、CAT、T-SOD、T-AOC含量升高(P<0.05),量效关系显著,由此可知,山楂粗糖蛋白对高脂小鼠具有良好的降脂作用和抗氧化活性。

关键词： 山楂; 粗糖蛋白; 高脂小鼠; 降脂; 抗氧化

本文引用格式

高雪婧 , 罗思奕 , 汤凯洁 , 罗秋水 . 山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究[J]. 食品与发酵工业, 2021 , 47(1) : 138 -142 . DOI: 10.13995/j.cnki.11-1802/ts.024873

Abstract

The hypolipidemic and antioxidant activity of crude glycoprotein from hawthorn in high-fat mice were investigated in the paper.The active ingredients were extracted by water extraction and alcohol precipitation, the free protein was removed by Sevage method, DEAE-52 cellulose chromatography and SephadexG-75 purification were used to prepare crude hawthorn glycoprotein.KM mice were selected as the research objects, and randomly divided into blank control group, high-fat model group, hawthorn glycoprotein low, medium and high dose groups.After 8 weeks of gavage, the content of total cholesterol (TC), triglyceride (TG) and high density lipoprotein cholesterol (HDL-C) in serum were measured, the activities of catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and the content of malonaldehyde (MDA) in liver were measured.Results showed that compared with the blank control group, the levels of TC, TG and MDA in the high-fat model group increased, while HDL-C, CAT, T-SOD and T-AOC decreased, all the differences were significant (P<0.01).Compared with the high-fat model group, the content of TC, TG and MDA in the hawthorn glycoprotein group decreased (P<0.05), and the content of HDL-C and the activities of CAT, T-SOD and T-AOC increased (P<0.05).The dose-effect relationship was significant, from this we can find that the crude glycoprotein from hawthorn had a better effect on hypolipidemic and antioxidant activity in high-fat mice.

Key words： hawthorn; crude glycoprotein; high-fat mice; hypolipidemic; antioxidant activity

参考文献

[1] 李晓梅,郭曙光,康文艺.山楂提取物的体外和体内抗氧化作用[J].精细化工,2009,26(4): 348-350.
LI X M,GUO S G,KANG W Y.Antioxidation of hawthorn extracts in vitro and in vivo[J].Fine Chemicals,2009,26(4):348-350.
[2] 王代明. 山楂提取物调节血脂作用的实验研究[J].中医药临床杂志,2012,24(12):1 147-1 148.
WANG D M.Experimental study on the regulating effect of hawthorn extract on blood lipid[J].Clinical Journal of Traditional Chinese Medicine,2012,24(12):1 147-1 148.
[3] 张玉颖, 张晾.山楂对低密度脂蛋白受体基因敲除小鼠脂代谢的影响[J].西安交通大学学报(医学版),2014,35(1):120-123.
ZHANG Y Y,ZHANG L.Effects of hawthornfruit on lipid metabolism in low density lipoprotein receptor-deficient mice[J].Journal of Xi’an Jiaotong University (Medical Sciences),2014,35(1):120-123.
[4] 王玲, 吴军林,吴清平,等.山楂降血脂作用和机理研究进展[J].食品科学,2015,36(15):245-248.
WANG L,WU J L,WU Q P,et al.A review of the lipid-lowering activity and mechanism of fructus crataegi[J].Food Science,2015,36(15):245-248.
[5] 高居易, 陈彦.建宁莲子糖蛋白的分离、纯化及清除自由基作用[J].武汉植物学研究,2003,21(2):175-178.
GAO J Y,CHEN Y.Isolation,purification of glycoproteins from seed of Nelumbo nucifera Gaertn.Fujian and its effect on scavenging free radicals[J].Plant Science Journal,2003,21(2):175-178.
[6] MATSUDA T,WATANADE K,NAKAMURA R.Immunochemical and physical properties of peptic-digested ovomucoid[J].Journal of Agricultural and Food Chemistry,1983,31(5):942-946.
[7] MATSUDA T,GU J,TSURUTA K,et al.Immunoreactive glycopeptides separated from peptic hydrolysate of chicken egg white ovomucoid[J].Journal of Food Science,1985,50(3):592-594.
[8] KOGA T,KIKUCHI M.Isolation and characterization of a novel immunomodulating fraction from soybeans[J].Bioscience,Biotechnology,and Biochemistry,1993,57(3):367-371.
[9] 张彩梅, 张红梅,于业军,等.扇贝多肽对小鼠免疫功能调节的研究[J].中国海洋药物杂志,2005,24(3):18-21.
ZHANG C M,ZHANG H M,YU Y J,et al.Immunomodulation of polypeptides from Chlamys farreri (PCF) in mice[J].Chinese Journal of Marine Drugs,2005,24(3):18-21.
[10] 马岩, 曹瑞敏,叶菲.亚侧耳硷溶性多糖蛋白的抗肿瘤作用[J].肿瘤,1998(1):3-5.
MA Y,CAO R M,YE F.Anti-tumor effect of alkaline-soluble polyglycoprotein of subpleurotus[J].Tumor,1998(1):3-5.
[11] 李亚娜, 阚建全,陈宗道,等.甘薯糖蛋白的降血脂功能[J].营养学报,2002(4):433-434.
LI Y N,KAN J Q,CHEN Z D,et al.Antilipidemic effect of glycoprotein from sweet potato[J].Acta Nutrimenta Sinica,2002(4):433-434.
[12] 白冰瑶, 周茜,韩雪,等.山楂浓缩汁对高脂小鼠的拮抗作用及其机制初探[J].食品科技,2017,42(4):67-72.
BAI B Y,ZHOU X,HAN X,et al.Concentrated hawthorn juice on antagonism and mechanism probation in mice with hyperlipidemia[J].Food Science and Technology,2017,42(4):67-72.
[13] 程江华, 王苗苗,王灼琛,等.复合降脂减肥固体饮料对大鼠营养性肥胖和高脂血症预防作用研究[J].现代食品科技,2016,32(12):6-12;85.
CHENG J H,WANG M M,WANG Z C,et al.Preventive effect of solid beverage on nutritional obesity and hyperlipidemia in rat fed with high-fat diet[J].Modern Food Science and Technology,2016,32(12):6-12;85.
[14] 罗秋水, 上官新晨,蒋艳,等.超声波辅助提取紫红薯糖蛋白工艺的优化[J].中国食品学报,2013,13(3):110-114.
LUO Q S,SHANGGUAN X C,JIANG Y,et al.Optimization on ultrasonic-assisted extracting glycoprotein from the purple sweet potato[J].Journal of Chinese Institute of Food Science and Technology,2013,13(3):110-114.
[15] 胡文兵, 赵静,陈婷婷,等.青钱柳多糖对高脂血症小鼠的降血脂作用及机制初探[J].现代食品科技,2015,31(11):39-44.
HU W B,ZHAO J,CHEN T T,et al.Preliminary studies on the hypolipidemic effect and mechanism of Cyclocarya paliurus polysaccharides in hyperlipidemic mice[J].Modern Food Science and Technology,2015,31(11):39-44.
[16] 陈显兵, 朱旻玥,唐凤,等.茼蒿对2型糖尿病大鼠糖脂代谢及抗氧化作用的影响[J].营养学报,2015,37(6):579-583.
CHEN X B,ZHU M Y,TANG F,et al.Effects of garland chrysanthemum on glucose and lipid metabolisms and antioxidant ability in type 2 diabetes rats[J].Acta Nutrimenta Sinica,2015,37(6):579-583.
[17] 王素贞, 黄占旺,黄永平,等.纳豆粉与红曲粉组合对高脂小鼠的降脂效果[J].食品工业科技,2018,39(14):292-296;305.
WANG S Z,HUANG Z W,HUANG Y P,et al.Lipid-lowering effect of natto and monascus mixture in hyperlipidemic mice[J].Science and Technology of Food Industry,2018,39(14):292-296;305.
[18] 马小灵,刘洁,王彦花,等.富硒茶油对小鼠降血脂和抗氧化作用的影响[J].中国粮油学报,2017,32(7):84-90.
MA X L,LIY J,WANG Y H,et al.Hypolipidemic and antioxidant effects of selenium-enriched camellia oil on mice[J].Journal of the Chinese Cereals and Oils Association,2017,32(7):84-90.
[19] 罗磊, 张冰洁,关宁宁,等.金银花叶黄酮对衰老模型小鼠的体内抗氧化作用[J].食品科学,2017,38(19):171-176.
LUO L,ZHANG B J,GUAN N N,et al.In vivo antioxidant activity of honeysuckle leaf flavonoids in aging mice[J].Food Science,2017,38(19):171-176.
[20] LI N,OUYANG K H,CAI J Y,et al.Research on anti-oxidant activity and hypolipemic mechanism of aloes flavonoids in mice[J].Journal of Food and Nutrition Research,2014,2(9):601-607.
[21] WANG X,LI W,XIAO L,et al.In vivo antihyperlipidemic and antioxidant activity of porphyran in hyperlipidemic mice[J].Carbohydrate Polymers,2017,174:417-420.
[22] LI W,JIANG N,LI B,et al.Antioxidant activity of purified ulvan in hyperlipidemic mice[J].International Journal of Biological Macromolecules,2018,113:971-975.
[23] XU N,REN Z,ZHANG J,et al.Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.tuoliensis[J].International Journal of Biological Macromolecules,2017,95:204-214.
[24] HONG Y,WU H,MA T,et al.Effects of glycyrrhiza glabra polysaccharides on immune and antioxidant activities in high-fat mice[J].International Journal of Biological Macromolecules,2009,45(1):61-64.
[25] VALKO M,LEIBFRITZ D,MONCOL J,et al.Free radicals and antioxidants in normal physiological functions and human disease[J].The International Journal of Biochemistry & Cell Biology,2007,39(1):44-84.
[26] 杨文字, 江春艳,严冬,等.以青稞β-葡聚糖为主要基质的保健果冻研制[J].食品科学,2012,33(4):296-300.
YANG W Z,JIANG C Y,YAN D,et al.Development of functional jelly using Tibetan hulless barley β-glucan as main matrix material[J].Food Science,2012,33(4):296-300.
[27] 董道顺. 木瓜、山楂复合型保健酸奶的研制[J].贵州农业科学,2013,40(12):187-190.
DONG D S.Development of health yoghurt mixed with pawpaw and hawthorn[J].Guizhou Agricultural Sciences,2013,40(12):187-190.
[28] SUN H C,LIU D Y,AN S,et al.Research on processing technique of hawthorn fruit and blackcurrant compound functional beverage[J].Advanced Materials Research,2013,807-809:1 999-2 002.
[29] 林标声, 罗茂春.蛹虫草-山楂复合保健饮料的研制[J].食品科学,2013,34(4):293-297.
LIN B S,LUO M C.Production of composite healthy beverage of Cordyceps militaris and hawthorn[J].Food Science,2013,34(4):293-297.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司