为探究羧甲基化木聚糖作为益生元的潜能,采用NaOH-氯乙酸反应体系对木聚糖进行羧甲基化修饰,红外光谱进行结构表征,模拟人体消化环境探究其抗消化性,并利用嗜热链球菌、德氏乳杆菌保加利亚亚种、植物乳杆菌和短乳杆菌几种益生菌对其益生元作用进行研究。结果表明,羧甲基化木聚糖在1 609、1 405、1 320 cm-1左右有特征吸收峰,表明羧甲基化修饰成功,且取代度为0.68;在模拟唾液、胃液和小肠液的消化实验中,羧甲基化木聚糖的水解度分别低于9%、5%和6%,表明其能抵抗人体消化道消化;羧甲基化木聚糖对4种供试菌的生长均有促进作用,且随着其浓度的增加而增大;羧甲基化木聚糖对益生菌生长繁殖的最适质量浓度为30 g/L,在24~36 h能达到生长最大量。以上结果表明羧甲基化木聚糖是一种潜在的益生元。
To explore the potential of carboxymethyl xylan as a prebiotics,we modified by sodium hydroxide chloroacetic acid reaction system was used to carboxymethylate xylan,and the structure was characterized by infrared spectroscopy.Its anti-digestibility was explored by simulating the human digestive environment,and its prebiotic effects on four probiotics (Lactobacillus brevis,Lactobacillus plantarum, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophiles) were studied.The results showed that carboxymethyl xylan had characteristic absorption peak at 1 609 cm-1、1 405 cm-1 and 1 320 cm-1,which indicated that it was successfully modified and the degree of substitution was 0.68.In the digestion experiments of simulated saliva,gastric juice and intestinal fluid,the hydrolysis degree of carboxymethyl xylan was lower than 9%,5% and 6%,respectively,indicating its resistance to digestion in human digestive tract.Moreover,carboxymethyl xylan could promote the growth of four probiotics and the promoting effect increased with its concentration.The optimum concentration of carboxymethyl xylan for the growth and reproduction of probiotics was 3%,reaching the maximum growth within 24-36 hours.Carboxymethyl xylan has the potential to be used as prebiotics.
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