食品与发酵工业 >

2021 , Vol. 47 >Issue 3: 107 - 113

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024655

生产与科研应用

利用蜂蜜接合酵母合成海藻糖

  • 张敏倩 ,
  • 刘功良 ,
  • 费永涛 ,
  • 白卫东 ,
  • 艾连中 ,
  • 俞剑燊
展开
  • 1(仲恺农业工程学院 轻工食品学院,广东 广州,510225)
    2(上海理工大学 医疗器械与食品学院,上海,200093)
    3(上海金枫酒业股份有限公司,上海,200120)
硕士研究生(刘功良教授为通讯作者,E-mail:gongliangliu@126.com)

收稿日期: 2020-06-05

  修回日期: 2020-07-05

  网络出版日期: 2021-03-08

基金资助

广东省重点领域研发计划项目(2018B020206001);广东省2019年省级农业科技创新及推广项目(2019KJ101)

收起

Synthesis of trehalose by Zygosaccharomyces mellis

  • ZHANG Minqian ,
  • LIU Gongliang ,
  • FEI Yongtao ,
  • BAI Weidong ,
  • AI Lianzhong ,
  • YU Jianshen
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  • 1(College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)
    2(School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    3(Shanghai Jinfeng Wine Co.Ltd., Shanghai 200120, China)

Received date: 2020-06-05

  Revised date: 2020-07-05

  Online published: 2021-03-08

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摘要

利用蜂蜜接合酵母LGL-2可耐受高糖浓度、应激代谢合成海藻糖的特性,研究了不同发酵时间、接种量、培养温度、葡萄糖初始质量浓度、转速对蜂蜜接合酵母代谢积累产生海藻糖的影响,并通过响应面优化了发酵工艺参数。结果表明,在发酵96 h、接种量10%(体积分数)、葡萄糖初始质量浓度300 g/L、温度24 ℃、转速180 r/min的条件下,海藻糖的质量分数可达92.32 mg/g干酵母,相比优化前提高了29.34%,该研究为蜂蜜接合酵母的应用及海藻糖的生物合成提供了借鉴。

关键词： 蜂蜜接合酵母; 海藻糖; 响应面优化; 发酵工艺

本文引用格式

张敏倩 , 刘功良 , 费永涛 , 白卫东 , 艾连中 , 俞剑燊 . 利用蜂蜜接合酵母合成海藻糖[J]. 食品与发酵工业, 2021 , 47(3) : 107 -113 . DOI: 10.13995/j.cnki.11-1802/ts.024655

Abstract

At present, research on the synthesis of trehalose with food grade microorganisms has attracted much attention. Since Zygosaccharomyces mellis LGL-2 can tolerate high glucose concentration and synthesize trehalose through stress metabolism, the effects of fermentation time, inoculation amount, culture temperature, initial concentration of glucose and rotational speed on trehalose production by LGL-2 were studied. The optimal fermentation conditions were determined by response surface methodology. The optimal conditions were determined as follows: 96 h, 10% inoculation size, 300 g/L initial glucose concentration, 24 ℃ and 180 r/min. Under the optimized conditions, the yield of trehalose was 92.32 mg/g dry yeast, which was 29.34% higher than that before optimization. The results provide reference for the application of Z. mellis and the biosynthesis of trehalose.

Key words： Zygosaccharomyces mellis; trehalose; response surface optimization; fermentation process

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