A species-specific PCR identification method was established to identify five kinds of animal-derived components of pork, beef, mutton, chicken and duck in long-term high-temperature processed foods such as canned meat. The total DNA templates of five kinds of animals were amplified by PCR. Then the amplified products of 212 bp, 147 bp, 202 bp, 131 bp, and 201 bp were obtained. Moreover, the sequencing results were verified by using the BLAST comparison on NCBI to confirm the accuracy of the experiment, and the commercially available canned samples were also been tested. In this method, the five animal introduced species have good specificity, the detection sensitivity for derived components of pork, beef, mutton and duck could reach 1%, and the detection sensitivity for chicken-derived components was 2.5%. After testing the commercially available samples, it was clear that in 6.6% of the samples, the actual composition did not match the label. This method was easy to operate, low in cost, accurate, reliable, which could be widely used in the identification of five animal-derived components of pigs, cattle, sheep, chickens and ducks in canned meat and long-term high-temperature processed foods. Above all, it had extensive value in applications.
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