以不同干燥方式得到的西藏林芝产松口蘑干品为研究对象,采用电子鼻结合GC-MS 技术对自然晾干、冷冻干燥、真空干燥、热风烘干得到的松口蘑干品的风味进行比较分析。结果表明:4种干燥方式所得松茸样品的传感器响应轮廓大致相同,但响应强度有所差别,4个样品的雷达响应值大小依次为真空干燥>自然晾干>冷冻干燥>热风烘干,其中真空干燥样品的响应值最大,说明该干燥方式较为完整地保留了松茸的风味信息;通过主成分分析和DFA图可知电子鼻技术能够较好的覆盖4个样品的风味信息,且互不干扰,该技术可用于不同样品的区分及鉴别。通过GC-MS共检测到4个样品中的137 种化合物,其中自然干燥109种,特有化合物1种;热风干燥112种,特有化合物6种;冷冻干燥108,特有化合物1种;真空干燥109种,特有化合物7种。
The effect of drying methods including natural drying, hot-air drying, vacuum drying and freeze drying on flavor of Tricholoma matsutake fruiting bodies were analyzed by electronic nose combined with GC-MS method. The result showed that the response profiles of radar sensors of T. matsutake fruiting bodies processed by different methods were roughly the same, however, the response intensity was different. The radar response values of the samples were vacuum drying > natural drying > freeze drying > hot-air drying, wherein the response value of vacuum drying sample was the highest, indicating that vacuum drying had the best effect on preserving the flavor of T. matsutake. The principal component analysis and discriminant function analysis diagrams showed that the electronic nose technology could well cover the flavor information of the four samples without interference, and thus can be used to distinguish and identify different samples. The results of GC-MS showed that 137 compounds were found in T. matsutake fruiting bodies processed by different methods, among which 109 were found in naturally dried sample and one was peculiar; 112 in hot-air dried sample and six were peculiar; 108 in freeze-dried sample and one was peculiar; 109 were vacuum-dried and seven were peculiar.
[1] 刘振东,汪雯翰,李梁,等.林芝松口蘑抗肿瘤组分的筛选[J].食用菌学报,2017,24(2):75-78.
LIU Z D,WANG W H,LI L,et al.Inhibition of tumor-cell proliferation by extracts of Tricholoma matsutake fruit bodies from Linzhi,Tibet[J].Acta Edulis Fungi,2017,24(2):75-78.
[2] 薛蓓,罗章,刘振东,等.林芝松口蘑与红菇蜡伞子实体香气成分比较[J].食品与发酵工业,2019,45(6):225-230.
XUE B,LUO Z,LIU Z D,et al.Analysis of volatile flavor compounds in Tricholoma matsutake and Hygrophorus russula(Fr.)Quel.by using HS-SPME-GC-MS[J].Food and Fermentation Industries,2019,45(6):225-230.
[3] WAN C X,XU H Y,XUE F,et al.Antitumor effects of a refined glycoprotein isolated from Tricholoma matsutake mycelium on sarcoma 180 in mice[J].Chinese Pharmacological Bulletin,2003,19(12):1 439-1 440.
[4] KAWAMURA Y,MANABE M,KITTA K A.novel antitumorigenic protein from a mushroom,Tricholoma matsutake,induces molecular alterations which lead to apoptosis to cancer cells[J].Journal of Biological Macromolecules,2002,2(2):52-58.
[5] WEI Y,XU H,XU Z.Inhibition effect of glycoprotein MTS03 from the submerged mycelia of Tricholoma matsutake on proliferation in MCF-7 cells in vitro[J].Chinese Pharmaceutical Journal-Beijing,2005,40(20):1545.
[6] 张燕,李姣娥,赖于民,等.不同贮藏方式对松茸呼吸强度影响[J].食品工业,2019,40(2):55-58.
ZHANG Y,LI J E,LAI Y M,et al.Effect of different storage methods on respiration intensity of pine mushroom[J].The Food Industry,2019,40(2):55-58.
[7] 张沙沙,邓雅元,罗晓莉,等.减压处理对松茸保鲜的影响初探[J].食用菌学报,2015,22(1):68-72.
ZHANG S S,DENG Y Y,LUO X L,et al.Effects of exposure to hypobaric conditions on selected Tricholoma matsutake fruit body parameters[J].Acta Edulis Fungi,2015,22(1):68-72.
[8] 王悦. 松茸保鲜技术的研究[D].哈尔滨:东北林业大学,2013.
WANG Y.Study on fresh-keeping technology of Tricholoma Matsutake[D].Harbin:Northeast Forestry University,2013.
[9] 温泉,王锡昌.食用菌风味物质成分的研究及应用进展[J].长江大学学报 (自然版),2006(4):211-214.
WEN Q,WANG X C.Progress in flavor research of edible fungus and its application[J].Journal of Yangtze University,2006(4):211-214.
[10] 张沙沙,罗晓莉,何容,等.松茸风味物质研究进展及应用展望[J].农产品加工,2019(9):68-70.
ZHANG S S,LUO X L,HE R,et al.Research progress and application prospect of Tricholoma matsutake flavor[J].Farm Products Processing,2019(9):68-70.
[11] 李文,杨焱,陈万超,等.不同干燥方式对香菇含硫风味化合物的影响[J].食用菌学报,2018,25(4):71-79.
LI W,YANG Y,CHEN W C,et al.Effect of drying method on sulfur-containing components in Lentinula edodes[J].Acta Edulis Fungi,2018,25(4):71-79.
[12] 石芳,李瑶,杨雅轩,等.不同干燥方式对松茸品质的影响[J].食品科学,2018,39(5):141-147.
SHI F,LI Y,YANG Y X,et al.Effect of different drying methods on the quality of Tricholoma matsutake[J].Food Science,2018,39(5):141-147.