食品与发酵工业 >

2021 , Vol. 47 >Issue 6: 214 - 220

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025283

分析与检测

高效液相色谱法测定虾青素油中虾青素的含量

  • 孔凡华 ,
  • 徐佳佳 ,
  • 郭倩 ,
  • 白沙沙 ,
  • 李东 ,
  • 方从容 ,
  • 邱楠楠 ,
  • 崔亚娟
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  • 1(国家食品安全风险评估中心,国家卫生健康委员会食品安全风险评估重点实验室,北京,100021)
    2(北京市营养源研究所,北京,100069)
硕士,工程师(邱楠楠副研究员和崔亚娟研究员为通讯作者,E-mail:qiunannan@cfsa.net.cn;cuiyj66@163.com)

收稿日期: 2020-08-05

  修回日期: 2020-10-05

  网络出版日期: 2021-04-15

基金资助

十三五国家重点研发计划(2016YFD0400600)

收起

Determination of astaxanthin in astaxanthin oil by high performance liquid chromatography

  • KONG Fanhua ,
  • XU Jiajia ,
  • GUO Qian ,
  • BAI Shasha ,
  • LI Dong ,
  • FANG Congrong ,
  • QIU Nannan ,
  • CUI Yajuan
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  • 1(NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit of Chinese Academy of Medical Science, China National Center for Food Safety Risk Assessment, Beijing 100021, China)
    2(Beijing Institute of Nutrition Resources, Beijing 100069, China)

Received date: 2020-08-05

  Revised date: 2020-10-05

  Online published: 2021-04-15

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摘要

该研究建立了高效液相色谱法测定虾青素油中虾青素含量的分析方法。利用单因素实验,对虾青素油中虾青素的提取试剂、酶解时间进行选择,确定最佳前处理条件为37 ℃恒温水浴振荡酶解60 min,石油醚提取。色谱条件:YMCTM C30色谱柱(250 mm×4.6 mm,5 μm),柱温30 ℃,流动相为甲醇-甲基叔丁基醚梯度洗脱,流速1 mL/min,检测波长472 nm。结果表明,全反式虾青素在0.10～4.00 μg/mL范围内与峰面积有良好线性关系,精密度的相对标准偏差(relative standard deviation,RSD)为1.36%,表明精密度高,平均回收率为93.58%,回收率的RSD为2.84%,表明添加回收率高,相对标准偏差小,实验建立方法测得虾青素油中虾青素的含量为92.9%,是碱性皂化测得结果的2.11倍,所建立的方法可以准确定量虾青素油中虾青素的含量。

关键词： 高效液相色谱法; 虾青素油; 虾青素; 全反式虾青素; 9-顺-虾青素; 13-顺-虾青素

本文引用格式

孔凡华 , 徐佳佳 , 郭倩 , 白沙沙 , 李东 , 方从容 , 邱楠楠 , 崔亚娟 . 高效液相色谱法测定虾青素油中虾青素的含量[J]. 食品与发酵工业, 2021 , 47(6) : 214 -220 . DOI: 10.13995/j.cnki.11-1802/ts.025283

Abstract

An analytical method was developed for the detection of astaxanthins in astaxanthin oil. The extraction reagent and enzymolysis time of astaxanthins in astaxanthin oil were selected by single factor experiment. The optimized pretreatment condition was bath shaking zymolytic for 60 min at 37 ℃ and petroleum ether extraction were selected for the extraction. The separation of astaxanthins were achieved on YMCTM C30 column(250 mm×4.6 mm, 5μm)using methanol-methyl tert-butyl ether as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set at 472 nm and 30 ℃, respectively. The results showed that the linear range of all trans astaxanthin was 0.10-4.00 μg/mL. The relative standard deviation (RSD) of astaxanthin in astaxanthin oil was 1.36%. Moreover, the average recovery rate of astaxanthin was 93.58% and RSD was 2.84%, indicating high recovery rate and small relative standard deviation. The astaxanthin content in astaxanthin oil by this method is 92.9% which was 2.11 times of alkaline saponification content. Therefore, the method established can accurately quantify astaxanthin content in astaxanthin oil.

Key words： high performance liquid chromatography(HPLC); astaxanthin oil; astaxanthin; all trans astaxanthin; 9-cis-astaxanthin; 13-cis-astaxanthin

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