食品与发酵工业 >

2021 , Vol. 47 >Issue 7: 109 - 115

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025314

研究报告

厚壳贻贝多糖对葡聚糖硫酸钠诱导的结肠炎改善作用

  • 姚丽文 ,
  • 周宇芳 ,
  • 孙继鹏 ,
  • 王家星 ,
  • 廖妙飞 ,
  • 郑斌 ,
  • 王芮 ,
  • 邓尚贵 ,
  • 相兴伟
展开
  • 1(浙江海洋大学 食品与医药学院,浙江 舟山,316021)
    2(浙江省海洋开发研究院,浙江 舟山,316021)
    3(浙江工业大学 食品科学与工程学院,浙江 杭州,310014)
硕士研究生(邓尚贵教授和相兴伟教授为共同通讯作者,E-mail:dengshanggui@163.com;xxw11086@zjut.edu.cn)

收稿日期: 2020-08-09

  修回日期: 2020-09-08

  网络出版日期: 2021-05-20

基金资助

浙江省重点研发计划项目(2019C02076);舟山市“五大会战”科技项目(2017C12030);浙江省公益性项目(LGN18C200006);浙江省教育厅一般科研项目(Y202043175)

收起

Improving effect of mussel (Mytilus corusus) polysaccharide on dextran sodium sulfate-induced colitis

  • YAO Liwen ,
  • ZHOU Yufang ,
  • SUN Jipeng ,
  • Wang Jiaxing ,
  • LIAO Miaofei ,
  • ZHENG Bin ,
  • WANG Rui ,
  • DENG Shanggui ,
  • XIANG Xingwei
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  • 1(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316021,China)
    2(Zhejiang Marine Development Institute,Zhoushan 316021,China)
    3(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China)

Received date: 2020-08-09

  Revised date: 2020-09-08

  Online published: 2021-05-20

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摘要

为了探讨厚壳贻贝多糖(Mytilus coruscus polysaccharide,MP)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导的结肠炎的改善作用,试验设置空白组、模型组和MP组(300 mg/kg),在日常饮用水中给予质量分数3.5% DSS构建结肠炎模型,通过观察小鼠体态表征、肠道组织形态、测定肠道和血清细胞因子水平,研究MP对小鼠结肠炎的影响。结果表明:与模型组相比,MP组显著缓解了小鼠体重的降低、结肠长度的缩短(P<0.05),并降低疾病活动指数,改善炎性细胞浸润、腺体水肿以及杯状细胞的减少,MP组结肠组织中超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽还原酶(flutathione peroxidase,GSH-Px)以及血清中的GSH-Px活性显著增高(P<0.05),血清中的SOD有所升高但无显著性差异。结肠组织和血清中的肿瘤坏死因子-α含量显著降低(P<0.05),白细胞介素-6含量有所降低但无显著性差异。该研究证明MP能在一定程度上改善由DSS诱导的结肠炎。

关键词： 厚壳贻贝多糖; 结肠炎; 葡聚糖硫酸钠; 抗氧化; 炎症反应

本文引用格式

姚丽文 , 周宇芳 , 孙继鹏 , 王家星 , 廖妙飞 , 郑斌 , 王芮 , 邓尚贵 , 相兴伟 . 厚壳贻贝多糖对葡聚糖硫酸钠诱导的结肠炎改善作用[J]. 食品与发酵工业, 2021 , 47(7) : 109 -115 . DOI: 10.13995/j.cnki.11-1802/ts.025314

Abstract

In this experiment, three groups:blank control group, model group and Mytilus coruscus polysaccharide (MP) group (300 mg/kg) were set up to explore whether MP could improve colitis induced by dextran sodium sulfate (DSS).After 3.5% DSS was given in daily drinking water, the drug was administrated by gavage, and the improvement effects of MP on colitis in mice were studied by observing the body characterization of mice, intestinal tissue morphology, and determination of intestinal and serum cytokines.The results showed that compared with the model group, the MP group could significantly alleviate the weight loss of mice, the shortening of the colon length (P<0.05), and reduce the disease activity index, improve inflammatory cell infiltration, glandular edema and goblet cell cut back.Compared with the model group, the superoxide dismutase (SOD) and glutathione reductase (GSH-Px) in the colon tissue of the MP group and the GSH-Px activity in the serum were significantly increased (P<0.05), and the SOD in the serum increased but there was no significant difference.The content of inflammatory factor tumor necrosis factor-ɑ(TNF-ɑ)in colon tissue and serum was significantly reduced (P<0.05), and the content of interleukin-6(IL-6)was reduced but there was no significant difference.The results proved that the Mytilus coruscus polysaccharide could improve the colitis induced by DSS to a certain extent.

Key words： Mytilus coruscus polysaccharide; colitis; dextran sodium sulfate; antioxidant; inflammation

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