食品与发酵工业 >

2021 , Vol. 47 >Issue 8: 189 - 196

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025790

生产与科研应用

响应面优化黑果腺肋花楸汁澄清工艺及其抗氧化活性评价

  • 王子涵 ,
  • 向敏 ,
  • 徐茂 ,
  • 蒋和体
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  • (西南大学 食品科学学院,重庆,400715)
硕士研究生(蒋和体教授为通讯作者,E-mail:jheti@126.com)

网络出版日期: 2021-05-20

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Optimization of clarification process of Aornia melanocarpa juice by response surface methodology and its antioxidant activity evaluation

  • WANG Zihan ,
  • XIANG Min ,
  • XU Mao ,
  • JIANG Heti
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  • (College of Food Science, Southwest University, Chongqing 400715,China)

Online published: 2021-05-20

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摘要

该研究以壳聚糖为澄清剂,采用响应面法优化黑果腺肋花楸汁的澄清条件,筛选出最佳澄清工艺,并对其总黄酮、总酚、花色苷等活性成分进行测定,且通过测定DPPH自由基、羟自由基清除能力,Fe3+还原能力以及总抗氧化能力评价其体外抗氧化活性。结果表明,黑果腺肋花楸汁最佳澄清条件为壳聚糖添加量0.56 g/100mL,澄清温度49 ℃,澄清时间67 min,在此条件下其透光率可达到93.89%;黑果腺肋花楸清汁与原汁相比各成分含量发生显著降低(P＜0.05),且原汁的抗氧化能力总体上强于清汁。相关性分析表明,黑果腺肋花楸清汁中的总酚和总黄酮含量与清汁DPPH自由基清除率、羟自由基清除率、Fe3+还原能力和总抗氧化能力呈现较好的相关性。研究结果显示,采用响应面法可优化黑果腺肋花楸汁的澄清工艺,提高汁液透光率,且黑果腺肋花楸汁具有较强的抗氧化活性,该研究为黑果腺肋花楸汁的实际开发利用提供数据支撑和理论基础。

关键词： 黑果腺肋花楸; 澄清工艺; 响应面法; 抗氧化活性; 相关性分析

本文引用格式

王子涵 , 向敏 , 徐茂 , 蒋和体 . 响应面优化黑果腺肋花楸汁澄清工艺及其抗氧化活性评价[J]. 食品与发酵工业, 2021 , 47(8) : 189 -196 . DOI: 10.13995/j.cnki.11-1802/ts.025790

Abstract

In order to explore the best clarification technology, quality and antioxidant activity of Aornia melanocarpa juice, response surface methodology (RSM) was used to optimize the clarification conditions of Aornia melanocarpa juice. And the active components, such as total flavonoids, total phenols and anthocyanins, were measured. Moreover, the antioxidant activity in vitro was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical clearance capability, Fe3+reduction capacity and total antioxidant capacity. The results showed that under the best clarification conditions, which was chitosan at the concentration of 0.56 g/100mL and with the treatment of clarification temperature 49 ℃ for 67 min, the light transmittance could reach 93.89%. Compared with the Aornia melanocarpa original juice, the content of each component in the clear juice was significantly reduced (P<0.05), and the antioxidant capacity of the original juice was stronger than that of the clear juice. Furthermore, the correlation analysis showed that the contents of total phenols and total flavonoids in the clear Aornia melanocarpa juice had a good correlation with DPPH free radical scavenging rate, hydroxyl radical scavenging rate, Fe3+reduction ability and total antioxidant capacity. In conclusion, RSM could optimize the clarification technology and improve the light transmittance of Aornia melanocarpa juice, and the juice has strong antioxidant activity, which provides data support and theoretical basis for the practical development and utilization of Aornia melanocarpa juice in the future.

Key words： Aornia melanocarpa; clarifying technology; response surface methodology; antioxidants; correlation analysis

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