食品与发酵工业 >

2021 , Vol. 47 >Issue 8: 224 - 229

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026155

分析与检测

基于气相色谱-串联质谱技术测定植物组织中糖与糖醇

  • 坚乃丹 ,
  • 李文丽 ,
  • 张祝莉 ,
  • 朱霞 ,
  • 鲜学海 ,
  • 牛伊宁
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  • 1(甘肃省干旱生境作物学重点实验室,甘肃 兰州,730070)
    2(临夏市农产品质量安全中心,甘肃 临夏,731100)
硕士,助理实验员(牛伊宁副研究员为通讯作者,E-mail:niuyn@gsau.edu.cn)

网络出版日期: 2021-05-20

基金资助

甘肃农业大学干旱生境作物学重点实验室基金课题资助(GSCS-2020-Z2);2020年甘肃省中央引导地方科技发展专项

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Determination of sugar and sugar alcohol in plant tissues by gas chromatography-tandem mass spectrometry

  • JIAN Naidan ,
  • LI Wenli ,
  • ZHANG Zhuli ,
  • ZHU Xia ,
  • XIAN Xuehai ,
  • NIU Yining
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  • 1(Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China)
    2(Linxia Agricultural Product Quality and Safety Center, Linxia 731100, China)

Online published: 2021-05-20

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摘要

该研究以植物组织中含量极少的侧金盏花醇作为内标物,建立气相色谱-串联质谱联用法(gas chromatography-tandem mass spectrometry,GC-MS/MS)同时检测植物组织中葡萄糖、半乳糖、肌醇、甘露醇、山梨醇、蔗糖的分析方法。样品采用氯仿-甲醇水溶剂提取法,加入吡啶、盐酸羟胺和乙酸酐衍生化,经HP-5MS毛细管色谱柱分离后使用电子轰击离子源以多重反应监测(multiple reaction monitoring,MRM)模式进行质谱检测。结果表明,各目标化合物在15 min内完成分离分析,6种乙酰化糖和糖醇检测响应呈现良好的线性关系,相关系数(correlation coefficients,R2)均在0.997以上,方法定量限(limits of quantitation, LOQs)为0.045～0.37 mg/L,检出限(limits of detection,LODs)为0.013～0.12 mg/L。植物样品加标回收率为95.38％～102.6％,相对标准偏差为1.28％～3.42％。该方法样品用量少,准确度高,精密度好,适合植物组织中微量糖和糖醇化合物含量的快速检测。

关键词： 糖与糖醇; 衍生; 乙酸酐; 气相色谱-串联质谱; 多重反应监测

本文引用格式

坚乃丹 , 李文丽 , 张祝莉 , 朱霞 , 鲜学海 , 牛伊宁 . 基于气相色谱-串联质谱技术测定植物组织中糖与糖醇[J]. 食品与发酵工业, 2021 , 47(8) : 224 -229 . DOI: 10.13995/j.cnki.11-1802/ts.026155

Abstract

To enhance the detection sensitivity of plant endogenous sugars and sugar alcohols, the quantitative analysis of multi-components by gas chromatography-tandem mass spectrometry (GC-MS/MS) for the determination of glucose, galactose, inositol, mannitol, sorbitol and sucrose was established by using adonitol as an internal standard substance. The sample was extracted by organic solvent and then mixed with pyridine, hydroxylamine hydrochloride and acetic anhydride for derivatization. The gas chromatography separation was performed on an HP-5MS capillary column. And the acetylated sugar and sugar alcohols were determined by MS in multiple reaction monitoring(MRM)mode. The results showed that, in the acquisition mode of mass spectrometry, there was a good linear relationship between the concentration of 6 acetylated sugars and sugar alcohols and detection response which with a correlation coefficients(R2) greater than 0.997. The detection limits (LODs) and quantification(LOQs) were 0.013-0.12 mg/L and 0.045-0.37 mg/L, respectively. The sample recovery rates ranged from 95.38％-102.6％, and the relative standard deviations were 1.28％-3.42％.The method has the advantages of low sample dosage, high accuracy, good precision, and thus could be successfully applied to the analysis of sugars and sugar alcohols in plant tissues at a low concentration.

Key words： sugar and sugar alcohol; derivatization; acetic anhydride; gas chromatography-tandem mass spectrometry; multiple reaction monitoring

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