食品与发酵工业 >

2021 , Vol. 47 >Issue 9: 114 - 119

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026008

研究报告

草莓叶水提物对成纤维细胞合成Ⅰ型胶原及分泌骨形态发生蛋白-1的影响

  • 丁文玉 ,
  • 何聪芬 ,
  • 刘蕾 ,
  • 杨笑笑 ,
  • 董坤
展开
  • 1(北京工商大学 化学与材料工程学院,北京,100048)
    2(北京市植物资源研究开发重点实验室(北京工商大学),北京,100048)
硕士研究生(董坤讲师为通讯作者,E-mail:dongkun@btbu.edu.cn)

收稿日期: 2020-10-26

  修回日期: 2020-12-03

  网络出版日期: 2021-06-03

基金资助

北京市科技计划资助项目(Z181100002418014);北京市教委科研计划一般资助项目(KM201810011007)

收起

Effects of water extractions from strawberry leaves on COL I synthesis and BMP-1 secretion in HFF-1

  • DING Wenyu ,
  • HE Congfen ,
  • LIU Lei ,
  • YANG Xiaoxiao ,
  • Dong Kun
Expand
  • 1(School of Chemical and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China)
    2(Beijing Key Lab of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China)

Received date: 2020-10-26

  Revised date: 2020-12-03

  Online published: 2021-06-03

Fold

摘要

草莓叶水提物(water extractions from strawberry leaves,SLWE)富含多种生物活性成分,能促进成纤维细胞(fibroblasts,FB)合成胶原蛋白,而胶原蛋白只有经过骨形态发生蛋白1(bone morphogenetic protein 1,BMP-1)的剪切才能变成有活性的蛋白。该文通过对SLWE主成分分析及主要酶活力测定,同时探究SLWE对人包皮成纤维细胞(human foreskin fibroblasts,HFF-1)合成胶原蛋白I(collagen I,COL I)及分泌BMP-1的影响。结果表明:SLWE中维生素C、原花青素、多糖、总黄酮、总酚、蛋白质的含量分别为(0.88 ±0.025)、(0.28 ±0.027)、(10.00 ±0.278)、(1.47 ±0.052)、(2.05 ±0.031)、(0.10 ±0.003)mg/mL;超氧化物歧化酶、过氧化物酶的酶活力分别为125.59、0.06 U/mL;SLWE对HFF-1细胞增殖作用不明显,但具有促进其COL I表达和增加BMP-1分泌量的作用。实验结果表明SLWE含有丰富的活性成分,具有促进HFF-1中COL I分泌和成熟的作用,且此功效并非通过增加细胞数量而实现的。综上所述,SLWE具备作为延缓衰老化妆品的功效成分的潜能,为草莓叶等废弃物资源再利用提供了理论依据。

关键词： 草莓叶水提物; 人包皮成纤维细胞; 胶原蛋白I; 骨形态发生蛋白1; 延缓衰老

本文引用格式

丁文玉 , 何聪芬 , 刘蕾 , 杨笑笑 , 董坤 . 草莓叶水提物对成纤维细胞合成Ⅰ型胶原及分泌骨形态发生蛋白-1的影响[J]. 食品与发酵工业, 2021 , 47(9) : 114 -119 . DOI: 10.13995/j.cnki.11-1802/ts.026008

Abstract

Water extractions from strawberry leaves(SLWE)contains a variety of bioactive components,which can stimulate fibroblasts to synthesize collagens.And collagens must be proteolytic cleavaged by bone morphogenetic protein 1(BMP-1) to turn into an active form.In this study,the bioactive component contents and main antioxidant enzyme activities of SLWE were analyzed.Furthermore,the effect of SLWE on collagen I (COL I) synthesis and BMP-1 secretion by human foreskin fibroblasts (HFF-1) was evaluated.The results showed that the contents of Vc,proanthocyanidins,polysaccharides,total flavonoids,total phenols and protein in SLWE were (0.88±0.025),(0.28±0.027),(10.00±0.278),(0.47±0.031),(0.10±0.003)mg/mL,respectively.SLWE also presented antioxidant activity with 125.59 U/mL,0.06 U/mL for SOD and POD,respectively.Though SLWE had no obvious effect on the proliferation of HFF-1 cells,it promoted COL I synthesis and secretion of BMP-1.Overall,our study indicated that SLWE contained abundant bioactive components and promoted the secretion and maturation of collagens in HFF-1 without increasing the cell number of HFF-1.Therefore,SLWE has been demonstrated as a potential functional ingredient for anti-aging cosmetics.Our research also provides theoretical basis for the reuse of agricultural wastes like strawberry leaves.

Key words： water extractions from strawberry leaves; human foreskin fibroblasts; collagen I; bone morphogenetic protein 1; anti-aging

参考文献

[1] 王佳曼,兰海梅.胶原及其产物在皮肤老化及皮肤修复中的应用[J].中国美容医学,2012,21(1):176-178.
WANG J M,LAN H M.The application of collagen and collagen hydrolysates in skin aging and skin repairing [J].Chinese Journal of Aesthetic Medicine,2012,21(1):176-178.
[2] 周聪,常新.骨形态发生蛋白-1的研究进展[J].大连医科大学报,2011,33(2):182-186.
ZOU C,CHANG X.Research progress of bone morphogenetic protein-1[J].Journal of Dalian Medical University,2011,33(2):182-186.
[3] HOPKINS D R,KELES S,GREENSPAN D S.The bone morphogenetic protein 1/Tolloid-like metalloproteinases[J].Matrix Biology,2007,26(7):508-523.
[4] 翟文丽,何聪芬,董坤.草莓叶中主要活性成分及其生物活性研究进展[J].北方园艺,2019(19):114-121.
ZHAI W L,HE C F,DONG K.Research progress in main active components and biological activities of strawberry leaves [J].Northern Horticulture,2019(19):114-121.
[5] KÅRLUND A,HANHINEVA K,LEHTONEN M,et al.Non-targeted metabolite profiling highlights the potential of strawberry leaves as a resource for specific bioactive compounds[J].Journal of the Science of Food and Agriculture,2017,97(7):2 182-2 190.
[6] LEE D S,KIM K H,YOOK H S.Cosmetic effects of the fractional extracts from strawberry (Fragaria ananassa var.‘Seolhyang') leaf[J].Journal of the Korean Society of Food Science and Nutrition,2018,47(3),271-278.
[7] ZHU Q C,NAKAGAWA T,KISHIKAWA A,et al.In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria ananassa var.Amaou)[J].Journal of Functional Foods,2015,13:38-49.
[8] ECHARD A.Strawberry extract for a radiant complexion[J].Pure Health Magazine,2012,18.
[9] 陈金娥,李冬梅,赵戌利,等.10种生、熟蔬菜中Vc含量及抗氧化性对比研究[J].食品科技,2012,37(4):57-59;64.
CHEN J E,LI D M,ZHAO X L,et al.Comparative of vitamin C detecting and antioxidant capability on 10 kinds of raw and cooked vegetables [J].Food Science and Technology,2012,37(4):57-59;64.
[10] 杨雪.草莓保鲜方法与茎叶中原花青素提取工艺的研究[D].成都:四川师范大学,2014.
YANG X.Study on strawberry preservation and extraction methods of proanthoc vanidins from stems and leaves [D].Chengdu:Sichuan Normal University,2014.
[11] CHAVAN J J,GAIKWAD N B,KSHIRSAGAR P R,et al.Total phenolics,flavonoids and antioxidant properties of three Ceropegia species from Western Ghats of India[J].South African Journal of Botany,2013,88(5):273-277.
[12] AL-GHUDANI M K N,HOSSAIN M A.Determination of total phenolics,flavonoids and antioxidant activity of root crude extracts of Adenium obesum traditionally used for the treatment of bone dislocations and rheumatism[J].Asian Pacific Journal of Tropical Disease,2015,5:S155-S158.
[13] 魏彦珍,邵兴锋,韦莹莹,等.茶树精油电辅助加热熏蒸处理对草莓品质和相关酶类的影响[J].食品与发酵工业,2017,43(4):135-140.
WEI Y Z,SHAO X F,WEI Y Y,et al.Effect of tea tree oil electric auxiliary heating fumigation on quality of strawberry fruit and its related enzymes[J].Food and Fermentation Industries,2017,43(4):135-140.
[14] 李慧,蒋丹丹,MORGAN D S,等.紫茉莉花提取物促进胶原蛋白功能化的功效研究[J].日用化学工业,2019,49(8):526-530.
LI H,JIANG D D,MORGAN D S,et al.Efficacy evaluation of Mirabilis jalapa flower extract on promoting collagen functionalization[J].China Surfactant Detergent & Cosmetics,2019,49(8):526-530.
[15] 高静.天然抗氧化剂及其协同作用[J].食品安全质量检测学报,2020,11(6):1 859-1 864.
GAO J.Natural antioxidants and synergistic effects[J].Journal of Food Safety and Quality,2020,11(6):1 859-1 864.
[16] 黄晓辉,茹晶晶,罗五魁,等.葡萄籽中原花青素的抗氧化性能的研究[J].宁德师范学院学报(自然科学版),2015,27(2):136-139.
HUANG X H,RU J J,LUO W K,et al.The study of antioxidation on procyanidins from grape seeds [J].Journal of Ningde Normal University (Natural Science),2015,27(2):136-139.
[17] 余正萍,陈林.天然产物银杏类黄酮的抗氧化活性研究[J].广东化工,2020,47(12):74-75.
YU Z P,CHEN L.The activity of antioxidant of natural product gingko flavonoids[J].Guangdong Chemical Industry,2020,47(12):74-75.
[18] 张慧.原花青素与维生素C的协同抗氧化作用的研究[J].山东化工,2016,45(9):24-25.
ZHANG H.Study on synergistic effect of the original anthocyanin and vitamin C combination on antioxidant activity[J].Shandong Chemical Industry,2016,45(9):24-25.
[19] 罗学兵,贺良明.草莓的营养价值与保健功能[J].中国食物与营养,2011,17(4):74-76.
LUO X B,HE L M.Nutritional value and health function of strawberry[J].Food and Nutrition in China,2011,17(4):74-76.
[20] 桑雅丽,李晓春,王欣宇,等.山葡萄籽中原花青素的提取及其含量测定[J].赤峰学院学报(自然科学版),2018,34(9):40-42.
SANG Y L,LI C X,WANG X Y,et al.Extraction and determination of proanthocyanidin from grape seeds[J].Journal of Chifeng University(Natural Science Edition),2018,34(9):40-42.
[21] 孙丽平,李鹏程,庄永亮,等.辣木叶多酚提取物对光老化小鼠皮肤的保护作用[J].食品与发酵工业,2020,46(18):67-71.
SUN L P,LI P C,ZHUANG Y L,et al.Protective effects of Moringa oleifera leaves polyphenol extracts on photoaging mice skin [J].Food and Fermentation Industries,2020,46(18):67-71.
[22] 张兵兵. 葡萄籽原花青素的提取、精制及性质研究[D].广州:华南理工大学,2017.
ZHANG B B.Study on extraction,purification and properties of proanthocyanidins from grape seeds[D].Guangzhou:South China University of Technology,2017.
[23] GEESIN J C,DARR D,KAUFMAN R,et al.Ascorbic acid specifically increases type I and type III procollagen messenger RNA levels in human skin fibroblasts[J].Journal of Investigative Dermatology,1988,90:420-424.
[24] 张娜娜,曹洪战,芦春莲.营养物质维生素C对基因表达的调控[J].饲料博览,2016(9):15-17.
ZHANG N N,CAO H Z,LU C L.Nutrients vitamin C effect on the regulation of gene expression [J].Feed Review,2016(9):15-17.
[25] 范丽云.维生素C对体外培养的人皮肤成纤维细胞HAS-2,MMP-1基因表达的影响[D].天津:天津医科大学,2009.
FAN L Y.Effects of vitamin C on the expression of hyaluronic acid synthetase-2 gene matrix metalloproteinase-1 gene in human dermal fibroblasts in vitro[D].Tianjin:Tianjin Medical university,2009.
[26] VIOUX-CHAGNOLEAU C,LEJEUNE F,SOK J,et al.Reconstructed human skin:From photodamage to sunscreen photoprotection and anti-aging molecules[J].Journal of Dermatological Science Supplement,2006,2(1):S1-S12.
[27] 刘玲英.芦荟多糖对体外培养人成纤维细胞增殖及胶原合成与分泌影响[D].福州:福建医科大学,2009.
LIU L Y.Influence of the aloe vera polysaccharide on both proliferation and collagen synthesis and secretion cultured human fibroblast cells in vitro[D].Fuzhou:Fujian:Fujian Medical University,2009.
[28] VOLOSHENVUK T G,FOURNETT A C,GARDNER J D.PI3K/Akt signaling mediates increased BMP-1 expression in response to TNF-α and TGF-β1 in cardiac fibroblasts[J].Federation of American Societies for Experimental Biology,2011,25:1.
[29] 李金燕,沈洁凤.人参皂苷Rb1对皮肤胶原代谢的影响[J].海峡药学,2017,29(10):21-23.
LI J Y,SHEN J F.Skin collagen metabolism regulated by ginsenoside Rb1[J].Strait Pharmaceutical Journal,2017,29(10):21-23.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司