食品与发酵工业 >

2021 , Vol. 47 >Issue 9: 154 - 159

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025692

生产与科研应用

屎肠球菌与植物乳杆菌共培养产γ-氨基丁酸条件优化及关键酶活性研究

  • 张恕铭 ,
  • 曾林 ,
  • 孙向阳 ,
  • 汪杰 ,
  • 孙擎 ,
  • 张庆 ,
  • 谭霄
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  • 1(西华大学 食品与生物工程学院,四川省食品生物技术重点实验室,四川 成都,610039)
    2(四川国检检测有限责任公司,四川 泸州,646000)
    3(中国科学院大学 中国科学院成都生物研究所,四川 成都,610041)
硕士研究生(张庆副教授为通讯作者,E-mail:biozhangq@163.com)

收稿日期: 2020-09-17

  修回日期: 2020-11-02

  网络出版日期: 2021-06-03

基金资助

四川省科技计划项目(2019ZYZF0170);四川省教育厅重点项目(18ZA0447)

收起

Optimization of γ-aminobutyric acid produced by co-culturing Enterococcus faecium and Lactobacillus plantarum and the activities of key enzyme

  • ZHANG Shuming ,
  • ZENG Lin ,
  • SUN Xiangyang ,
  • WANG Jie ,
  • SUN Qing ,
  • ZHANG Qing ,
  • TAN Xiao
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  • 1(Provincial Key Laboratory of Food Biotechnology of Sichuan,College of Food and Bioengineering, Xihua University,Chengdu 610039,China)
    2(Sichuan National Inspection and Testing Co.Ltd.,Luzhou 646000,China)
    3(Chengdu Institute of Biology,Chinese Academy of Science,Chengdu 610041,China)

Received date: 2020-09-17

  Revised date: 2020-11-02

  Online published: 2021-06-03

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摘要

为进一步提高γ-氨基丁酸(γ-aminobutyric acid,GABA)产量,以从泡菜中筛选获得的产GABA屎肠球菌(Enterococcus faecium)AB157与植物乳杆菌(Lactobacillus plantarum)BC112为研究对象,采用高效液相色谱法对菌株共培养发酵产GABA表达能力进行评估。通过单因素试验和响应面优化共培养条件,并在最优条件下对共培养体系和单菌发酵进行谷氨酸脱羧酶酶活力分析。结果表明,当L-谷氨酸钠质量浓度为12.7 g/L、屎肠球菌AB157与植物乳杆菌BC112接种体积比例为5∶3、发酵时间85 h时,共培养产GABA能力最强,达6.35 g/L,较屎肠球菌AB157单菌株发酵产GABA(1.60 g/L)提高3.9倍。在此条件下分析谷氨酸脱羧酶活力,在发酵过程中,菌株共培养体系中谷氨酸脱羧酶活力明显高于单一屎肠球菌AB157发酵酶活力。

关键词： 屎肠球菌; γ-氨基丁酸; 植物乳杆菌; 共培养; 谷氨酸脱羧酶

本文引用格式

张恕铭 , 曾林 , 孙向阳 , 汪杰 , 孙擎 , 张庆 , 谭霄 . 屎肠球菌与植物乳杆菌共培养产γ-氨基丁酸条件优化及关键酶活性研究[J]. 食品与发酵工业, 2021 , 47(9) : 154 -159 . DOI: 10.13995/j.cnki.11-1802/ts.025692

Abstract

In order to further improve production of γ-aminobutyric acid (GABA),the GABA-producing strains of Enterococcus faecium AB157 and Lactobacillus plantarum BC112 selected from Paocai (Chinese pickle) were analyzed.And the GABA expression of co-culture fermentation broth was evaluated by high performance liquid chromatography (HPLC).Response surface methodology was used to optimize the co-culture conditions.The activity of key glutamate decarboxylase in the co-culture system was also analyzed.The results showed that the optimal co-culture fermentation conditions were 12.7 g/L of L-glutamate sodium,5∶3 inoculation volume ratio of E.faecium AB157 to L.plantarum BC112 and 85 h.Also,a yield of 6.35 g/L GABA which was 3.9 times (1.60 g/L) higher than that of single fermentation with E.faecium AB157 was achieved in the co-culture.Meanwhile,in the co-culture system,the activity of glutamate decarboxylase was significantly higher than the enzyme activity of E.faecium AB157 single strain.

Key words： Enterococcus faecium; γ-aminobutyric acid; Lactobacillus plantarum; co-culture; glutamate decarboxylase

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