通过研究测试菌株大肠埃希氏菌(Escherichia coli)CICC 24176和CICC 24652在不同培养基上的生长特性,以评价其质控食品微生物学检验培养基的可行性。依据GB 4789.28—2013中定量、半定量及定性方法, ATCC 25922作为对照菌株,确认2株测试菌株在非选择性分离和计数固体培养基、选择性分离和计数固体培养基、选择性增菌培养基、选择性液体计数培养基和鉴定培养基上共6类58种培养基上的生长率、选择性及特异性。结果表明,菌株CICC 24176和CICC 24652在分离和计数固体培养基上生长率均>0.7,在选择性液体计数培养基上的生长情况良好,在选择性固体培养基和悬浮培养基上生长被有效抑制,在特异性培养基上均具有典型生化特征;菌株CICC 24176在除SC液体培养基外的8种选择性增菌培养基上被有效抑制,而CICC 24652在所有选择性增菌培养基上均被有效抑制;菌株CICC 24176在18种鉴定培养基上具有典型生化特征,而菌株CICC 24652在16种鉴定培养基上具有典型生化特征,SIM(hydrogen sulfide indole motility)动力培养基及缓冲动力硝酸盐培养基上动力呈阴性。综上,2株测试菌株可分别用于相应培养基微生物性能指标的质量评价。
刘佳奇
,
都海渤
,
陈怡文
,
张彩文
,
辛迪
,
石靓
,
李金霞
,
崔生辉
,
姚粟
. 大肠埃希氏菌(Escherichia coli)用于食品检验培养基质量控制的研究[J]. 食品与发酵工业, 2021
, 47(10)
: 79
-86
.
DOI: 10.13995/j.cnki.11-1802/ts.025740
The growth characteristics of test strains Escherichia coli CICC 24176 and CICC 24652 on different medium were studied to evaluate the feasibility of quality control for medium in food microbiological examination. According to the national standards GB 4789.28—2013, the growth rate, selectivity and specificity of these two test strains were determined on specified media, and ATCC 25922 was used as the control strain. The media was consisted of 58 kinds of medium which can be divided into six types: non-selective separation and counting solid medium, selective separation and counting solid medium, selective enrichment medium, selective liquid counting medium, suspension medium and identification medium. Results showed that the growth rates of strain CICC 24176 and CICC 24652 on separation and counting solid medium were all greater than 0.7. Strains grew well on selective liquid counting medium, and growth was effectively inhibited on selective solid and suspension medium. And typical biochemical characteristics were observed on specific medium. The strain CICC 24176 was effectively inhibited on 8 kinds of selective enrichment media except SC liquid medium, while CICC 24652 was effectively inhibited on all selective enrichment media. Strain CICC 24176 had typical biochemical characteristics on 18 kinds of identification media, while strain CICC 24652 had typical biochemical characteristics on 16 kinds of identification media except for SIM medium and motility nitric acid test medium. In conclusion, the test strains can be used as quality evaluation of the microbial performance indicators on corresponding medium.
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