食品与发酵工业 >

2021 , Vol. 47 >Issue 10: 185 - 191

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025890

生产与科研应用

球毛壳菌CGMCC 6882利用小麦秸秆发酵生产抗氧化多糖

  • 王子朝 ,
  • 郭佳源 ,
  • 郜文硕 ,
  • 崔静雯 ,
  • 杨青青 ,
  • 朱金帆 ,
  • 张慧茹
展开
  • 1(河南工业大学 生物工程学院,河南 郑州,450001)
    2(河南工业大学 国际教育学院,河南 郑州,450001)
博士,讲师(本文通讯作者,E-mail:6832917@163.com)

收稿日期: 2020-10-14

  修回日期: 2020-11-24

  网络出版日期: 2021-06-17

基金资助

河南省高等学校重点科研计划(19A180015);河南省谷物资源转化与利用重点实验室开放课题(PL2018006);河南工业大学高层次人才基金项目(2017BS011)

收起

Production of an antioxidant polysaccharide from wheat straw by Chaetomium globosum CGMCC 6882

  • WANG Zichao ,
  • GUO Jiayuan ,
  • GAO Wenshuo ,
  • CUI Jingwen ,
  • YANG Qingqing ,
  • ZHU Jinfan ,
  • ZHANG Huiru
Expand
  • 1(College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China)
    2(School of International Education, Henan University of Technology, Zhengzhou 450001, China)

Received date: 2020-10-14

  Revised date: 2020-11-24

  Online published: 2021-06-17

Fold

摘要

针对我国小麦秸秆存在严重浪费和污染环境的现状,该实验采用球毛壳菌CGMCC 6882对小麦秸秆进行发酵,生产一种新型胞外多糖,并对多糖结构和抗氧化活性进行研究。结果显示,球毛壳菌CGMCC 6882以小麦秸秆为碳源发酵所得胞外多糖的单糖组成和摩尔比为鼠李糖∶氨基葡萄糖∶半乳糖∶葡萄糖∶木糖∶果糖∶葡萄糖醛酸=21.46∶1.58∶1.11∶55.15∶36.37∶7.04∶7.34,重均分子质量Mw为3.538×104 Da。体外抗氧化实验结果显示,球毛壳菌CGMCC 6882胞外多糖可以有效清除DPPH自由基、羟自由基、超氧阴离子和ABTS阳离子自由基,且清除率与多糖浓度成正相关。多糖质量浓度为5 mg/mL时,对上述自由基的清除率分别达到(83.08±2.58)%、(81.39±1.47)%、(79.38±2.03)%和(85.69±1.62)%。细胞抗氧化实验结果显示,加入多糖实验组的丙二醛酶活水平从(15.8±0.25) μmol/g降低至(7.5±0.31) μmol/g,与多糖浓度成负相关;Caco-2细胞内超氧化物岐化酶、过氧化氢酶和谷胱甘肽过氧化物酶活力水平分别从(147.28±4.82)、(11±1.15)和(18.08±1.96) U/mg增加至(238.89±3.91)、(39.12±0.76)和(41.35±2.18) U/mg,且与多糖浓度成正相关。该实验为小麦秸秆的高效利用提供了重要依据和理论基础。

关键词： 小麦秸秆; 球毛壳菌CGMCC 6882; 多糖; 结构特征; 抗氧化活性

本文引用格式

王子朝 , 郭佳源 , 郜文硕 , 崔静雯 , 杨青青 , 朱金帆 , 张慧茹 . 球毛壳菌CGMCC 6882利用小麦秸秆发酵生产抗氧化多糖[J]. 食品与发酵工业, 2021 , 47(10) : 185 -191 . DOI: 10.13995/j.cnki.11-1802/ts.025890

Abstract

Due to the serious resource waste and environmental pollution induced by wheat straw in China, wheat straw was used by Chaetomium globosum CGMCC 6882 to produce exopolysaccharide in present work. Meanwhile, the structure of the exopolysaccharide and its antioxidant activity were investigated. Results showed that monosaccharide composition of the polysaccharide produced by C. globosum CGMCC 6882 was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46∶1.58∶1.11∶55.15∶36.37∶7.04∶7.34, and its molecular weight was 3.538×104 Da. Antioxidant assays in vitro revealed that the polysaccharide had strong scavenging effects on DPPH radical, hydroxyl radical, superoxide anions and ABTS radical in a dose-dependent manner. At the concentration of 5 mg/mL, the corresponding scavenging abilities of the polysaccharide were (83.08±2.58)%, (81.39±1.47)%, (79.38±2.03)% and (85.69±1.62)%, respectively. Results of cell antioxidant test showed that the activity of MDA decreased from (15.8±0.25) μmol/g to (7.5±0.31) μmol/g, which was negatively correlated with the concentration of polysaccharide. Furthermore, the activity levels of SOD, CAT and GSH-Px in H2O2 induced Caco-2 cells increased from (147.28±4.82), (11±1.15) and (18.08±1.96) U/mg to (238.89±3.91), (39.12±0.76) and (41.35±2.18) U/mg, and those were positively correlated with the concentration of polysaccharide. Overall, this work provides an important reference and theoretical basis for the efficient utilization of wheat straw.

Key words： wheat straw; Chaetomium globosum CGMCC 6882; polysaccharide; structural feature; antioxidant activity

参考文献

[1] 高利伟, 马林, 张卫峰, 等.中国作物秸秆养分资源数量估算及其利用状况[J].农业工程学报, 2009(7):173-179.
GAO L W, MA L, ZHANG W F, et al.Estimation of nutrient resource quantity of crop straw and its utilization situation in China[J].Transactions of the Chinese Society of Agricultural Engineering, 2009(7):173-179.
[2] LIU T, ZHOU X Q, LI Z F, et al.Effects of liquid digestate pretreatment on biogas production for anaerobic digestion of wheat straw[J].Bioresource Technology, 2019, 280:345-351.
[3] ISLAM S M M, ELLIOTT J R, JU L K.Minimization of fermentation inhibitor generation by carbon dioxide-water based pretreatment and enzyme hydrolysis of guayule biomass[J].Bioresource Technology, 2018, 251:84-92.
[4] 陈凤, 赵万岭, 王星仪, 等.苯酚-硫酸法测定明日叶根中多糖含量[J].化工管理, 2019(26):11-13.
CHEN F, ZHAO W L, WANG X Y, et al.Determination of polysaccharide content in Angelica keiskei root by phenol sulfuric acid method[J].Chemical Enterprise Management, 2019(26):11-13.
[5] HU X L, WANG K L, YU M, et al.Characterization and antioxidant activity of a low molecular weight xanthan gum[J].Biomolecules, 2019, 9(11):730.
[6] 郭增旺, 樊乃境, 田海芝, 等.小米糠黄酮对H2O2致HepG2氧化应激损伤的保护作用[J].食品科学, 2020, 41(5):159-165.
GUO Z W, FAN N J, TIAN H Z, et al.Protective effects of flavonoids from millet bran on H2O2-induced oxidative stress injury in HepG2 cells[J].Food Science, 2020, 41(5):159-165.
[7] XIANG Y L, XU X L, LI J.Chemical properties and antioxidant activity of exopolysaccharides fractions from mycelial culture of Inonotus obliquus in a ground corn stover medium[J].Food Chemistry, 2012, 134(4):1 899-1 905.
[8] XU X Q, HU Y, QUAN L L.Production of bioactive polysaccharides by Inonotus obliquus under submerged fermentation supplemented with lignocellulosic biomass and their antioxidant activity[J].Bioprocess and Biosystems Engineering, 2014, 37(12):2 483-2 492.
[9] LIU X C, PANG H, GAO Z, et al.Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus[J].International Journal of Biological Macromolecules, 2019, 131:315-322.
[10] SONG X L, SHEN Q, LIU M, et al.Antioxidant and hepatoprotective effects of intracellular mycelium polysaccharides from Pleurotus geesteranus against alcoholic liver diseases[J].International Journal of Biological Macromolecules, 2018, 114:979-988.
[11] LI B, ZHANG N, WANG D X, et al.Structural analysis and antioxidant activities of neutral polysaccharide isolated from Epimedium koreanum Nakai[J].Carbohydrate Polymers, 2018, 196:246-253.
[12] 景永帅, 张钰炜, 李佳瑛, 等.硒多糖的合成方法、结构特征和生物活性研究进展[J/OL].食品工业科技,2021.DOI:10.13386/j.issn1002-0306.2020050188.
JING Y S, ZHANG Y W, LI J Y, et al.Progress of synthesis methods, structural characteristics and biological activities of selenium polysaccharides[J/OL].Science and Technology of Food Industry, 2021.DOI:10.13386/j.issn1002-0306.2020050188.
[13] BLASCHEK W, KÄSBAUER J, KRAUS J, et al.Pythium aphanidermatum:Culture, cell-wall composition, and isolation and structure of antitumour storage and solubilised cell-wall (1→3),(1→6)-β-D-glucans[J].Carbohydrate Research, 1992, 231:293-307.
[14] ZHANG M, ZHANG L N, CHEUNG P C K, et al.Molecular weight and anti-tumor activity of the water-soluble polysaccharides isolated by hot water and ultrasonic treatment from the sclerotia and mycelia of Pleurotus tuber-regium[J].Carbohydrate Polymers, 2004, 56(2):123-128.
[15] WANG Z C, CHEN P Z, NING T, et al.Anticancer activity of polysaccharides produced from glycerol and crude glycerol by an endophytic fungus Chaetomium globosum CGMCC 6882 on human lung cancer A549 cells[J].Biomolecules, 2018, 8(4):171.
[16] LO T C T, CHANG C A, CHIU K H, et al.Correlation evaluation of antioxidant properties on the monosaccharide components and glycosyl linkages of polysaccharide with different measuring methods[J].Carbohydrate Polymers, 2011, 86(1):320-327.
[17] MIRZADEH M, ARIANEJAD M R, KHEDMAT L.Antioxidant, antiradical, and antimicrobial activities of polysaccharides obtained by microwave-assisted extraction method:A review[J].Carbohydrate Polymers, 2020, 229:115 421.
[18] SHEN S G, JIA S R, WU Y K, et al.Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme[J].Carbohydrate Polymers, 2018, 198:426-433.
[19] NAWROCKA A, SZYMAN′SKA-CHARGOT M, MIS′ A, et al.Effect of dietary fibre polysaccharides on structure and thermal properties of gluten proteins-A study on gluten dough with application of FT-Raman spectroscopy, TGA and DSC[J].Food Hydrocolloids, 2017, 69:410-421.
[20] JEONG H K, LEE D, KIM H P, et al.Structure analysis and antioxidant activities of an amylopectin-type polysaccharide isolated from dried fruits of Terminalia chebula[J].Carbohydrate Polymers, 2019, 211:100-108.
[21] 王浩南, 吴雨蒙, 张永清, 等.丹参花与根部多糖抗氧化活性及其组成研究[J].辽宁中医药大学学报, 2020, 22(11):38-43.
WANG H N, WU Y M, ZHANG Y Q, et al.Analysis of monosaccharide composition and antioxidant activity of Salvia miltiorrhiza and Salvia miltiorrhiza polysaccharides[J].Journal of Liaoning University of Traditional Chinese Medicine, 2020, 22(11): 38-43.
[22] 张佳婵, 王昌涛, 易思雨, 等.沙棘粕提取物对H2O2诱导的B16F10细胞氧化损伤的保护及修复[J].中国食品学报, 2019, 19(8):13-21.
ZHANG J C,WANG C T,YI S Y,et al.Protective and prothetic effects of Sea buckthorn seed extracts on H2O2-induced oxidative damage of B16F10[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(8):13-21.
[23] 吴丽红, 怀雪, 马智超, 等.川牛膝多糖对H2O2诱导PC12细胞氧化损伤保护作用的研究[J].化学工程师, 2019, 33(2):68-70;49.
WU L H, HUAI X, MA Z C, et al.Protection of polysaccharids from Cyathula officinalis Kuan on PC12 cells injury induced by H2O2[J].Chemical Engineer, 2019,33(2):68-70;49.
[24] 顾程远, 陈秋利, 李海涛.桔梗多糖对H2O2诱导PC12细胞氧化损伤的保护作用及机制研究[J].南京中医药大学学报, 2017, 33(3):268-272.
GU C Y, CHEN Q L, LI H T.Protective effects and mechanism of polysaccharide from Platycodon Grandiflorum on damage of PC12 cells induced by H2O2[J].Journal of Nanjing University of Traditional Chinese Medicine, 2017, 33(3):268-272.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司