A method for determination of 15 kinds of elements of K, Na, Ca, Mg, P, Fe, Mn, Cu, Zn, Al, As, Pb, Cd, Cr, Se in oyster sauce by inductively coupled plasma optical emission spectrometry/inductively coupled plasma mass spectrometry (ICP-OES/ICP-MS) was established, and the nutritive value and possible risks of health and safety were assessed. The samples were digested by nitric acid and hydrogen peroxide. The spectral interference of ICP-OES was eliminated by selecting different analysis lines, and the matrix effect of ICP-MS was diminished by adding internal standard elements of Ge, Rh, and Re online, and the mass spectral interference was diminished by kinetic energy discrimination (KED). In addition, scallop reference material (GBW 10024) was used to verify the accuracy of the method. The results showed that the detection limits of ICP-OES were 0.125-0.987 mg/kg with 89.3%-103.5% the average recoveries, and the relative standard deviations (RSD) were 0.2%-3.7%. While the detection limits of ICP-MS were 0.001-0.014 mg/kg, the average recoveries were 87.2%-107.8% and the RSD was 0.2%-2.7%. The linear relationship of standard curves was good (R2>0.999). The contents of macro-elements of K, Na, Ca, Mg in the oyster sauce were high, while heavy metals of As, Pb, Cd, Cr were low. This method is accurate, efficient and sensitive. It can meet the requirements for the determination of elements in oyster sauce.
[1] 江雄辉, 廖国洪.蚝油的生产技术[J].食品与机械, 2002(1):30-33.
JIANG X H, LIAO G H.Productive techniques of oyster sauce[J].Food and Machinery, 2002(1):30-33.
[2] 方玲, 马海霞, 李来好, 等.华南沿海地区近江牡蛎重金属污染调查与评价[J].食品与发酵工业, 2018, 44(5):237-243.
FANG L, MA H X, LI L H, et al.Investigation and assessment on heavy metals in ostrea rivularis from the coast of South China Sea[J].Food and Fermentation Industries, 2018, 44(5):237-243.
[3] JONG S M, HYUN D Y, POONG H K, et al.Bioaccumulation of heavy metals in oysters from southern coast of Korea:Assessment of potential risk of human health[J].Bulletin of Environmental Contamination and Toxicology, 2015, 6(94):749-755.
[4] 赵鹏, 张荣灿, 覃仙玲, 等.北部湾钦州港近江牡蛎重金属污染分析[J].水产学报, 2017, 41(5):806-815.
ZHAO P, ZHANG R C, QIN X L, et al.Evaluation of heavy metals in oyster (Crassostrea rivularis) from qinzhou harbor, Beibu Gulf[J].Journal of Fisheries of China, 2017, 41(5):806-815.
[5] 王增焕, 林钦, 王许诺, 等.华南沿海牡蛎重金属含量特征及其风险评估[J].水产学报, 2011, 35(2):291-297.
WANG Z H, LIN Q, WANG X N, et al.The variation features of heavy metal contents in oyster samples from the coast of South China sea and their safety assessment[J].Journal of Fisheries of China, 2011, 35(2):291-297.
[6] JAE Y J, PYO J P, WON K J, et al.Amino acid changes in fermented oyster (Crassostrea rivularis) sauce with different fermentation periods[J].Food Chemistry, 2005, 91(1):15-18.
[7] JAE Y J, JI Y P, WON K J, et al.Isolation of angiotensin I converting enzyme (ACE) inhibitor from fermented oyster sauce, Crassostrea gigas[J].Food Chemistry, 2005, 90(4):809-814.
[8] NGUYEN T H D, WANG X C.Volatile, taste components, and sensory characteristics of commercial brand oyster sauces:Comparisons and relationships[J].International Journal of Food Properties, 2012, 15(3):518-535.
[9] 潘婷, 杨雷, 朱科帆, 等.市售蚝油产品品质评价[J].食品工业科技, 2016, 37(20):96-100.
PANG T, YANG L, ZHU K F, et al.Quality evaluation of commercial oyster sauce samples[J].Science and Technology of Food Industry, 2016, 37(20):96-100.
[10] 刘丽, 杨艺超, 苏家琳, 等.多频脉冲电子舌技术对不同品种蚝油辨识的分析[J].食品与发酵科技, 2018, 54(2):109-119.
LIU L, YANG Y C, SU J L, et al.Identification of different kinds of oyster sauce by multi-frequency pulse electronic tongue technique[J].Food and Fermentation Science and Technology, 2018, 54(2):109-119.
[11] 邓瑞君, 徐荣雄.变性淀粉在蚝油中的应用比较[J].食品科技, 2014, 39(9):263-266.
DENG R J, XU R X.Compared of application of modified starch in oyster sauce[J].Food Science and Technology, 2014, 39(9):263-266.
[12] 韩硕, 郑姣姣, 李永歌.酸水解植物蛋白调味粉在蚝油中的应用[J].中国调味品, 2019, 44(1):136-140.
HAN S, ZHENG J J, LI Y G.Application of HVP powder in oyster sauce[J].China Condiment, 2019, 44(1):136-140.
[13] 赖国银, 林建忠, 曾琪, 等.凯氏定氮蒸馏装置-高效液相色谱法同时测定蚝油等食品中丙酸钠(钙)和双乙酸钠[J].食品科学, 2017, 38(4):270-274.
LAI G Y, LIN J Z, ZENG Q, et al.Simultaneous determination of sodium (or calcium) propionate and sodium diacetate in oyster sauce and other similar seasonings by high performance liquid chromatography using Kjeldahl distillation apparatus[J].Food Science, 2017, 38(4):270-274.
[14] 张丽华, 邵瑞婷, 杨丽梅, 等.超高效液相色谱串联质谱法测定蚝油中3种甲基咪唑类物质[J].食品与发酵工业, 2019, 45(24):229-233.
ZHANG L H, SHAO R T, YANG L M, et al.Determination of three methylimidazoles in oyster sauce using ultra-high performance liquid chromatography-tandem mass spectrometry[J].Food and Fermentation Industry, 2019, 45(24):229-233.
[15] 金科, TUSHAR R P, 李振兴, 等.蛤蜊壳羟基磷灰石用于脱除蚝油中铅的初步研究[J].中国渔业质量与标准, 2015, 5(6):54-58.
JIN K, TUSHAR R P, LI Z X, et al.Primary study on Pb removal in oyster sauce by hydroxyapatite of clam shell[J].Chinese Fishery Quality and Standards, 2015, 5(6):54-58.
[16] KWOK O W, YOCK H C, HUAY L S.3-Monochloropropane-1,2-diol (3-MCPD) in soy and oyster sauces:Occurrence and dietary intake assessment[J].Food Control, 2006, 17(5):408-413.
[17] 许培振, 丁筑红.扇贝体内部分金属元素含量分析[J].食品工业, 2016, 37(6):184-186.
XU P Z, DING Z H.Analysis of some metal elements in scallop[J].Food Industry, 2016, 37(6):184-186.
[18] 郝云彬, 宋洪强, 吴益春, 等.氢化物发生原子荧光法对贝类中无机砷监测及其安全性评价[J].食品科学, 2012, 33(4):219-222.
HAO Y B, SONG H Q, WU Y C, et al.Determination and safety evaluation of inorganic Arsenic in shellfish by hydride generation-atomic fluorescence spectrometry[J].Food Science, 2012, 33(4):219-222.
[19] 杨桂珍, 陈伟珍, 劳小周, 等.湛江常食用贝类中锰、铁、锌、铜四种元素的含量测定[J].食品研究与开发, 2014, 35(10):99-101.
YANG G Z, CHEN W Z, LAO X Z, et al.Determination of manganese, iron, zinc and copper elements in edible shellfish in Zhanjiang[J].Food Research and Development, 2014, 35(10):99-101.
[20] JASMINA D, THORJORN L, ARMIN S, et al.Contents of cadmium, copper, mercury and lead in fish from the Neretva river (Bosnia and Herzegovina) determined by inductively coupled plasma mass spectrometry (ICP-MS)[J].Food Chemistry, 2012, 131(2):469-476.
[21] GIRUM H, JI Y C, EUN Y N, et al.Determination of toxic heavy metal levels in commonly consumed species of shrimp and shellfish using ICP-MS/OES[J].Food Science and Biotechnology, 2015, 24(1):373-378.
[22] 赵艳芳, 段元慧, 尚德荣, 等.我国几种重要经济贝类中砷的含量及其形态特征转化规律[J].水产学报, 2013, 37(5):735-741.
ZHAO Y F, DUAN Y H, SHANG D R, et al.Arsenic content and speciation analysis of several economic shellfishes in China[J].Journal of Fisheries of China, 2013, 37(5):735-741.
