食品与发酵工业 >

2021 , Vol. 47 >Issue 11: 151 - 157

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026440

生产与科研应用

低共熔溶剂提取对黄精多糖性质及抗氧化活性的影响

  • 唐兰芳 ,
  • 王锋 ,
  • 苏小军 ,
  • 李清明 ,
  • 郭红英 ,
  • 孙毅中 ,
  • 孙翟翎
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  • 1(湖南农业大学 食品科学技术学院,湖南 长沙,410128)
    2(湖南省发酵食品工程技术研究中心,湖南 长沙,410128)
    3(新化县绿源农林科技有限公司,湖南 新化,417600)
硕士研究生(王锋副教授为通讯作者,E-mail: wangfeng@hunau.edu.cn)

收稿日期: 2020-12-14

  修回日期: 2021-02-01

  网络出版日期: 2021-07-16

基金资助

湖南省科技计划项目(2016NK2113;2019NK3010-02)

收起

Effects of deep eutectic solvents on the properties and antioxidant activity of polysaccharides from Polygonatum cyrtonema Hua

  • TANG Lanfang ,
  • WANG Feng ,
  • SU Xiaojun ,
  • LI Qingming ,
  • GUO Hongying ,
  • SUN Yizhong ,
  • SUN Zhailing
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  • 1(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China)
    2(Hunan Provincial Research Center of Engineering and Technology for Fermented Food,Changsha 410128,China)
    3(Agriculture and Forestry Technology Co.Ltd.of Xinhua city,Xinhua 417600,China)

Received date: 2020-12-14

  Revised date: 2021-02-01

  Online published: 2021-07-16

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摘要

为揭示低共熔溶剂对黄精多糖理化性质和抗氧化活性的影响规律,以多花黄精为原料,分别采用低共熔溶剂和热水提取黄精多糖,对多糖提取率与分子质量、单糖组成等基本性质进行分析,并比较2种方法提取的黄精多糖抗氧化能力和抗糖基化能力的差异。结果表明,采用低共熔溶剂提取黄精多糖,提取率是热水提取的3.40倍,所得多糖分子质量显著大于热水提取,单糖组成也更复杂。2种方法提取的黄精多糖均为吡喃糖,属于酸性多糖。扫描电镜结果显示,由低共熔溶剂提取的黄精多糖为光滑片状结构,而热水所提取的黄精多糖表面粗糙且为碎片状。氧自由基吸收能力、铁离子还原能力、DPPH自由基与ABTS阳离子自由基清除能力 4种抗氧化与抗糖基化试验测定结果均表明,采用低共熔溶剂法提取的黄精多糖抗氧化与抗糖基化能力显著高于热水提取法。2种方法提取的黄精多糖理化性质与生物活性差异显著,该文可为低共熔溶剂提取植物多糖提供参考。

关键词： 低共熔溶剂; 黄精多糖; 理化性质; 抗氧化; 抗糖基化

本文引用格式

唐兰芳 , 王锋 , 苏小军 , 李清明 , 郭红英 , 孙毅中 , 孙翟翎 . 低共熔溶剂提取对黄精多糖性质及抗氧化活性的影响[J]. 食品与发酵工业, 2021 , 47(11) : 151 -157 . DOI: 10.13995/j.cnki.11-1802/ts.026440

Abstract

The aim of this study was to reveal the influence of extracted polysaccharides with deep eutectic solvents from Polygonatum sibiricum on the properties and antioxidant activity. The Polygonatum cyrtonema Hua (PcH) was taken as raw materials and the polysaccharides were extracted with deep eutectic solvents (DESs) of choline chloride-urea (CCU) and hot water respectively. The extraction yield, properties and bioactivity of the polysaccharides from two methods were compared. The results showed that the yield of polysaccharides extracted with DESs was 3.40 times higher than that extracted with hot water. The polysaccharides extracted with DESs possessed higher molecular weight and their composition of monosaccharides were more complex. Moreover, Fourier transform infrared spectroscopy (FTIR) spectra showed that the polysaccharides obtained by the two methods were pyranose and acidic polysaccharides. And scanning electron microscope (SEM) indicated that the polysaccharides extracted with DESs was flaky and smooth, with hot water was fragmented and rough. More importantly, the oxygen radical absorbance capacity (ORAC), ferric ion reducing antioxidant power (FRAP), DPPH, ABTS tests showed that the polysaccharides extracted with DESs exhibit significantly higher antioxidant capacity and anti-glycosylation activity than hot water. The physicochemical properties and biological activity of polysaccharides obtained by two methods were significantly different. This paper can provide a reference for the extraction of polysaccharides from plants by deep eutectic solvents.

Key words： deep eutectic solvents; Polygonatum cyrtonema Hua polysaccharides; physicochemical property; antioxidant; anti-glycosylation

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