食品与发酵工业 >

2021 , Vol. 47 >Issue 13: 23 - 29

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026771

研究报告

低谷蛋白大米在仿生大肠反应器中对肠道菌群结构及代谢的影响

  • 胡国奥 ,
  • 詹晓北 ,
  • 李志涛 ,
  • 朱莉 ,
  • 赵志超 ,
  • 张洪涛
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  • 1(工业生物技术教育部重点实验室(江南大学),江苏 无锡,214125)
    2(无锡格莱克斯生物科技有限公司,江苏 无锡,214125)
    3(中国农业科学院作物科学研究所,北京,100081)
硕士研究生(詹晓北教授为通讯作者,E-mail: xbzhan@yahoo. com)

收稿日期: 2021-01-15

  修回日期: 2021-03-02

  网络出版日期: 2021-08-02

基金资助

十三五国家重点研发技术项目(2017YFD0400302)

收起

Effects of flow glutelin rice on composition and metabolism of intestinal flora in bionic large intestinal reactor

  • HU Guoao ,
  • ZHAN Xiaobei ,
  • LI Zhitao ,
  • ZHU Li ,
  • ZHAO Zhichao ,
  • ZHANG Hongtao
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  • 1(Key Laboratory of Industrial Biotechnology,Jiangnan University,Wuxi 214122,China)
    2(Wuxi Glycose Biotechnology Co.Ltd.,Wuxi 214125,China)
    3(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

Received date: 2021-01-15

  Revised date: 2021-03-02

  Online published: 2021-08-02

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摘要

以淀粉、低谷蛋白大米和普通大米为碳源,通过在仿生大肠反应器中对健康人群的粪便微生物进行发酵,探究低谷蛋白大米对人体肠道菌群结构和代谢的影响。结果表明,低谷蛋白大米的有益代谢产物中,短链脂肪酸(short chain fatty acids,SCFAs)的浓度达到104.85 mmol/L,比淀粉组和普通大米组提高44.94%和25.33%,乳酸的浓度达到18.19 mmol/L,比淀粉组和普通大米组提高60.55%和25.28%;低谷蛋白大米的有害代谢产物中,支链脂肪酸(branched chain fatty acids,BCFAs)的浓度为0.29 mmol/L,比普通大米组降低79.31%,氨的浓度为2.60 mmol/L,比普通大米组降低16.15%。平板计数显示,肠道有益菌拟杆菌和双歧杆菌均有显著增长(P<0.05)。16S rDNA测序显示,肠道有益菌拟杆菌门和厚壁菌门的丰度提高,肠道有害菌变形菌门和梭杆菌门的丰度降低。研究结果表明,低谷蛋白大米对健康人群肠道菌群的结构和代谢均具有正向影响。

关键词： 低谷蛋白大米; 仿生大肠反应器; 肠道菌群; 短链脂肪酸; 支链脂肪酸

本文引用格式

胡国奥 , 詹晓北 , 李志涛 , 朱莉 , 赵志超 , 张洪涛 . 低谷蛋白大米在仿生大肠反应器中对肠道菌群结构及代谢的影响[J]. 食品与发酵工业, 2021 , 47(13) : 23 -29 . DOI: 10.13995/j.cnki.11-1802/ts.026771

Abstract

As a new kind of functional food, the effects of low glutelin rice on intestinal flora after gastrointestinal digestion remained to be studied. Starch, low glutelin rice and ordinary rice were used as carbon sources. The fecal microorganisms of healthy people were fermented in a bionic large intestinal reactor, to explore the effects of low glutelin rice on the structure and metabolism of human intestinal flora. The results showed that in the beneficial metabolites of low glutelin rice, the concentration of short chain fatty acids(SCFAs)reached 104.85 mmol/L, which was 44.94% and 25.33% higher than starch and ordinary rice. The concentration of lactic acid reached 18.19 mmol/L, which was 60.55% and 25.28% higher than starch and ordinary rice. In the harmful metabolites of low glutelin rice, the concentration of branched chain fatty acids (BCFAs)was 0.29 mmol/L, which was 79.31% lower than ordinary rice. The concentration of ammonia was 2.60 mmol/L, which was 16.15% lower than ordinary rice. Plate counting showed that the beneficial intestinal bacteria Bacteroides and Bifidobacterium increased significantly (P<0.05). 16S rDNA sequencing showed that the abundance of Bacteroidetes and Firmicutes increased while that of Proteobacteria and Fusobacteria decreased. Based on the results, low glutelin rice has a positive effect on the structure and metabolism of intestinal flora in healthy people.

Key words： low glutelin rice; bionic large intestinal reactor; intestinal flora; short chain fatty acid; branched chain acid

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