为了解益生菌粉中的活菌稳定性,对6个市售益生菌粉产品中的益生菌存活数以及对模拟胃液、肠液及胃肠液的耐受性进行了分析。结果表明:6个产品中的活菌数与标识存在差异,产品E中活菌数最多,为10.19 lg CFU/g,产品B中活菌数最少,为6.47 lg CFU/g;20 ℃贮藏3个月后,产品F活菌数下降一个数量级,其他产品活菌数均维持稳定。6个产品在pH为4.5、3.5、2.5的模拟胃液中耐受能力较强,在pH为3.5模拟胃液中消化2 h后,存活率在91.08%~108.83%。经模拟肠液消化1 h后,益生菌存活率均显著下降(P＜0.05);消化2 h后,益生菌存活率在54.95%~97.43%。经过模拟胃肠道消化体系(pH为3.5的模拟胃液停留2 h后,再在模拟肠液将进行消化2 h)后,产品D的存活率最高(98.39%),产品F的存活率最低(60.93%)。不同产品中的活菌数及其对模拟消化液耐受性的差异较大,该研究结果可为进一步制定相关标准提供依据。
In order to study the stability of probiotics preparation, six commercial probiotics products were investigated for the living rates and their tolerance to simulated gastrointestinal environment. The results indicated that the viable counts were different from the label of the products. The number of live probiotics in E and B was the highest 10.19 lg CFU/g and the lowest 6.47 lg CFU/g, respectively,. The living probiotics counts in F decreased by an order of magnitude after storage for three months at 20 ℃, while others remained stable. All products were tolerant to simulated gastric fluid of pH 4.5, 3.5 and 2.5, and their survival rates were 91.08%-108.83% after a 2 h digestion at pH 3.5. The survival rate of the products decreased significantly (P<0.05) after a 1h digestion in simulated intestinal fluid, and their survival rate was 54.95%-97.43% after a 2 h digestion. Through the simulated gastrointestinal digestive system (simulated gastric fluid at pH 3.5 for 2 h, simulated intestinal fluid for 2 h), the survival rate of D was the highest (98.39%), and the lowest value (60.93%) was detected in F. Different probiotics powder showed great differences on viable counts and the tolerance to simulated digestive fluid. Therefore, this study could provide reference for the quality analysis of probiotics powder and basis for the establishment of related standards.
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