食品与发酵工业 >

2021 , Vol. 47 >Issue 13: 79 - 84

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026702

研究报告

体外模拟消化对牦牛骨胶原蛋白肽抗氧化活性的影响

  • 杨玉亮 ,
  • 衣大龙 ,
  • 刘春雨 ,
  • 辛瑜 ,
  • 顾正华 ,
  • 刘怀高 ,
  • 郭自涛 ,
  • 张梁
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  • 1(粮食发酵工艺与技术国家工程实验室(江南大学),江苏 无锡,214122)
    2(安徽国肽生物科技有限公司,安徽 宣城,242100)
硕士研究生(郭自涛博士和张梁教授为共同通讯作者,E-mail:hnpygzt@126.com;zhangl@jiangnan.edu.cn)

收稿日期: 2021-01-09

  修回日期: 2021-02-01

  网络出版日期: 2021-08-02

基金资助

轻工业技术与工程 国家双一流学科项目(LITE2018-22)

收起

Effects of in vitro simulated digestion on the antioxidant activity of yak bone collagen peptides

  • YANG Yuliang ,
  • YI Dalong ,
  • LIU Chunyu ,
  • XIN Yu ,
  • GU Zhenghua ,
  • LIU Huaigao ,
  • GUO Zitao ,
  • ZHANG Liang
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  • 1(National Engineering Laboratory for Cereal Fermentation Technology(Jiangnan University),Wuxi 214122,China)
    2(Anhui Guotai Biotechnology CO.Ltd.,Xuancheng 242100,China)

Received date: 2021-01-09

  Revised date: 2021-02-01

  Online published: 2021-08-02

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摘要

牦牛骨胶原蛋白经酶解产生的牦牛骨胶原蛋白肽有良好的抗氧化性,为评估其是否适合应用于营养品或保健品的开发,需要研究胃肠道消化对牦牛骨胶原蛋白肽抗氧化活性的影响。研究使用胃蛋白酶、胰液等物质对牦牛骨胶原蛋白肽进行体外模拟胃肠消化,采用水解度、分子质量分布、氨基酸组成对模拟消化前后牦牛骨胶原蛋白肽进行表征,并测定样品·OH清除能力、Fe2+螯合能力及ABTS阳离子自由基清除能力以评估模拟消化对样品抗氧化能力的影响。结果显示,经过体外模拟胃肠消化后,对抗氧化性能起决定性作用的疏水氨基酸含量并无显著变化。分子质量>1 000 Da的组分显著降低,且降幅超过50%;<500 Da的组分显著升高,分子质量介于180~1 000 Da的主体部分无明显变化。消化前后除·OH清除能力无明显差异外,其他抗氧化性均有提高,表明严苛的胃肠消化不能破坏牦牛骨的抗氧化性功能肽。因此牦牛骨胶原蛋白肽适合应用于营养品和保健品。

关键词： 牦牛骨胶原蛋白肽; 抗氧化性; 模拟胃肠消化; ·OH清除能力; Fe2+螯合能力; ABTS阳离子自由基清除率

本文引用格式

杨玉亮 , 衣大龙 , 刘春雨 , 辛瑜 , 顾正华 , 刘怀高 , 郭自涛 , 张梁 . 体外模拟消化对牦牛骨胶原蛋白肽抗氧化活性的影响[J]. 食品与发酵工业, 2021 , 47(13) : 79 -84 . DOI: 10.13995/j.cnki.11-1802/ts.026702

Abstract

Yak bone collagen peptide (YBCP) produced by enzymatic hydrolysis of yak collagen protein has good antioxidant activity. To evaluate whether it is suitable for the development of nutrition or health care products, it is necessary to study the effect of gastrointestinal digestion on the antioxidant activity of YBCP. In this study, the degree of hydrolysis, molecular weight distribution, the amino acid composition of YBCP was characterized before and after simulated digestion. Moreover, the ability of hydroxyl radicals (·OH) scavenging, Fe2 + chelating and ABTS+ scavenging was measured to evaluate the effects of simulation digestion on the antioxidant capacity of YBCP. The results showed that the contents of hydrophobic amino acids, which play a decisive role in antioxidation, however, did not change significantly after simulated gastrointestinal digestion. The molecular weight >1 000 Da components decreased significantly that the average reduction ratio was more than 50%. The composition<500 Da was significantly increased, and the main part of the molecular weight between 180-1 000 Da had no significant changes. There was no significant difference in ·OH scavenging ability before and after digestion, but other antioxidant activities were increased. This indicated that severe gastrointestinal digestion could not destroy the antioxidant functional YBCP. Therefore, YBCP is suitable for the application of nutrition and health products.

Key words： yak bone collagen peptide; antioxidant; simulate gastrointestinal digestion Hydroxyl radical scavenging ability; Fe2+ chelating ability; ABTS+ clearance

参考文献

[1] MAULIK S K,KUMAR S.Oxidative stress and cardiac hypertrophy:A review[J].Toxicology Mechanisms and Methods,2012,22(5):359-366.
[2] DALLE-DONNE I,ROSSI R,COLOMBO R,et al.Biomarkers of oxidative damage in human disease[J].Clinical Chemistry,2006,52(4):601-623.
[3] DALLE-DONNE I,GIUSTARINI D,COLOMBO R,et al.Protein carbonylation in human diseases[J].Trends in Molecular Medicine,2003,9(4):169-176.
[4] HARMAN D.Aging:A theory based on free radical and radiation chemistry[J].Journal of Gerontology,1956,11(3):298-300.
[5] 张雅楠, 梁鹏,谢静仪,等.天然食品抗氧化剂的研究进展[J].中国食物与营养,2019,25(1):67-71.
ZHANG Y N,LIANG P,XIE J Y,et al.Research advancements of natural food antioxidants[J].Food and Nutrition in China,2019,25(1):67-71.
[6] JIRATANAN T,LIU R H.Antioxidant activity of processed table beets (Beta vulgaris var.Conditiva) and green beans (Phaseolus vulgaris L.)[J].Journal of Agricultural and Food Chemistry,2004,52(9):2 659-2 670.
[7] SHI Y N,KOVACS-NOLAN J,JIANG B,et al.Peptides derived from eggshell membrane improve antioxidant enzyme activity and glutathione synthesis against oxidative damage in caco-2 cells[J].Journal of Functional Foods,2014,11:571-580.
[8] LIN S Y,WANG J,ZHAO P,et al.Optimized antioxidant peptides fractions preparation and secondary structure analysis by MIR[J].International Journal of Biological Macromolecules,2013,59:151-157.
[9] GU L J,ZHAO M M,LI W Z,et al.Chemical and cellular antioxidant activity of two novel peptides designed based on glutathione structure[J].Food and Chemical Toxicology,2012,50(11):4 085-4 091.
[10] YOUNG D,FAN M Z,MINE Y.Egg yolk peptides up-regulate glutathione synthesis and antioxidant enzyme activities in a porcine model of intestinal oxidative stress[J].Journal of Agricultural and Food Chemistry,2010,58(13):7 624-7 633.
[11] 李东东. 罗非鱼皮多肽抗皮肤光老化作用研究[D].湛江:广东海洋大学,2018.
LI D D.Study on anti-skin photoaging effect of tilapia skin collagen polypeptide[D].Zhanjiang:Guangdong Ocean University,2018.
[12] 丁树慧. 低值鱼低聚肽的酶法制备及抗氧化、抗疲劳活性研究[D].长春:吉林农业大学,2018.
DING S H.Studies on the enzymatic preparation of low value fish oligopeptides and its antioxidant and anti-fatigue activities[D].Changchun:Jilin Agricultural University,2018.
[13] 肖聪丽, 李理,陈敏.大豆多肽的分离纯化及其体外降血脂功能研究[J].中国酿造,2020,39(7):30-35.
XIAO C L,LI L,CHEN M,et al.Separation and purification of soybean peptides and its hypolipidemic function in vitro[J].China Brewing,2020,39(7):30-35.
[14] 倪蕴琪, 唐静仪,刘怀高,等.牦牛骨资源综合利用的研究现状与展望[J].农产品加工,2020(22):87-92.
NI Y Q,TANG J Y,LIU H G,et al.A summary review and advance of comprehensive utilization of yak bone resources[J].Farm Products Processing,2020(22):87-92.
[15] SÁNCHEZ-PATÁN F,CUEVA C,MONAGAS M,et al.In vitro fermentation of a red wine extract by human gut microbiota:changes in microbial groups and formation of phenolic metabolites[J].Journal of Agricultural and Food Chemistry,2012,60(9):2 136-2 147.
[16] 姜海洋, 张周莉,孙敏,等.牦牛骨抗氧化肽的分离纯化及鉴定[J].基因组学与应用生物学,2019,38(8):3 479-3 485.
JIANG H Y,ZHANG Z L,SUN M,et al.Isolation,purification and identification antioxidant peptides from yak bone[J].Genomics and Applied Biology,2019,38(8):3 479-3 485.
[17] SUN X H,WANG K,GAO S,et al.Purification and characterization of antioxidant peptides from yak (Bos grunniens) bone hydrolysates and evaluation of cellular antioxidant activity[J].Journal of Food Science and Technology,2020(3):1-14.
[18] 于小栋. 牦牛骨胶原蛋白肽的制备及其功能特性研究[D].西宁:青海师范大学,2019.
YU X D.Preparation and functional properties of yak bone collagen peptide[D].Xining:Qinghai Normal University,2019.
[19] 叶孟亮, 张春晖,等.牦牛骨胶原蛋白促成骨活性肽分离纯化及结构鉴定//中国食品科学技术学会.中国食品科学技术学会第十六届年会论文集[C].2019:55-56.
YE M L,ZHANG C H,et al.Isolation,purification and structure identification of active bone peptide facilitated by yak collagen//Chinese Institute of Food Science and Technology[C],2019:55-56.
[20] 芦鑫, 胡东彬,贾聪,等.体外模拟消化芝麻蛋白产生多肽的抗氧化性分析[J].中国油脂,2020,45(5):63-66;81.
LU X,HU D B,JIA C,et al.Antioxidant analysis of peptides produced by in vitro simulated digestion of sesame protein[J].China Oils and Fats,2020,45(5):63-66;81.
[21] GAO S,HONG H,ZHANG C Y,et al.Immunomodulatory effects of collagen hydrolysates from yak (Bos grunniens) bone on cyclophosphamide-induced immunosuppression in BALB/c mice[J].Journal of Functional Foods,2019,60:103 240.
[22] PUCHALSKA P,CONCEPCION GARCIA M,LUISA MARINA M.Identification of native angiotensin-I converting enzyme inhibitory peptides in commercial soybean based infant formulas using HPLC-Q-ToF-MS[J].Food Chemistry,2014,157:62-69.
[23] 王海萍.高固形物浓度大豆分离蛋白酶解特性的研究[D].广州:华南理工大学,2016.
WANG H P.The effect of high solid concentration on characteristics of enzymatic hydrolysis of soy protein isolates[D].Guangzhou:South China University of Technology,2016.
[24] YANG B,YANG H,LI J,et al.Amino acid composition,molecular weight distribution and antioxidant activity of protein hydrolysates of soy sauce lees[J].Food Chemistry,2011,124(2):551-555.
[25] 卢素珍. 鱼鳞明胶抗氧化肽的制备及其抗氧化作用研究[D].南昌:南昌大学,2019.
LU S Z.Preparation of fish scale gelatin antioxidant peptides and its antioxidant activity study[D].Nanchang:Nanchang University,2019.
[26] CHUNG Y C,CHANG C T,CHAO W W,et al.Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1[J].Journal Agricultural and Food Chemistry,2002,50(8):2 454-2 458.
[27] KETNAWA S,LICEAGA A M.Effect of microwave treatments on antioxidant activity and antigenicity of fish frame protein hydrolysates[J].Food and Bioprocess Technology,2017,10(3):582-591.
[28] FARVIN K H S,ANDERSEN L L,OTTE J,et al.Antioxidant activity of cod (Gadus morhua) protein hydrolysates:Fractionation and characterisation of peptide fractions[J].Food Chemistry,2016,204:409-419.
[29] 葛晓鸣, 顾伟,徐永健.海马水解蛋白的氨基酸组成与抗氧化能力的关系[J].核农学报,2019,33(2):322-329.
GE X M,GU W,XU Y J.Relationship between amino acid composition and antioxidant capacity of hydrolyzed protein from Hippocampus kuda bleeker[J].Journal of Nuclear Agricultural Sciences,2019,33(2):322-329.
[30] KIM S K,WIJESEKARA I.Marine-derived peptides:Development and health prospects marine proteins and peptides[M].Chichester:John Wiley & Sons Ltd,2013.
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