食品与发酵工业 >

2021 , Vol. 47 >Issue 13: 146 - 153

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026193

生产与科研应用·

蓝圆鲹黄嘌呤氧化酶抑制肽的制备及其活性分析

  • 周雅 ,
  • 胡晓 ,
  • 李来好 ,
  • 杨贤庆 ,
  • 陈胜军 ,
  • 吴燕燕 ,
  • 杨少玲
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(中国水产科学研究院 南海水产研究所,农业农村部水产品加工重点研究室,广东 广州,510300)
硕士研究生(胡晓副研究员为通讯作者,E-mail: hnhuxiao@163.com)

收稿日期: 2020-11-16

  修回日期: 2020-12-09

  网络出版日期: 2021-08-02

基金资助

财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-47);中国水产科学研究院基本科研业务费资助项目(2020TD69);广东省基础与应用基础研究基金项目(2019A1515011588);广东省科技创新战略专项资金(纵向协同管理方向)计划项目(2018S0044);广东省重点领域研发计划资助项目(2020B1111030004);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项(2021SD06)

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Preparation and characterization of xanthine oxidase inhibitory peptides from round scad (Decapterus maruadsi) muscleZ

  • HOU Ya ,
  • HU Xiao ,
  • LI Laihao ,
  • YANG Xianqing ,
  • CHEN Shengjun ,
  • WU Yanyan ,
  • YANG Shaoling
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  • 1(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
    2(Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China)

Received date: 2020-11-16

  Revised date: 2020-12-09

  Online published: 2021-08-02

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摘要

以蓝圆鲹为原料,选用胰蛋白酶、木瓜蛋白酶、中性蛋白酶、复合蛋白酶及碱性蛋白酶对其进行水解。以酶解产物黄嘌呤氧化酶(xanthine oxidase,XOD)的抑制活性为指标,通过单因素试验及正交试验确定了最佳的酶解制备条件。结果显示,最佳酶解条件为采用中性蛋白酶,加酶量0.3%(质量分数),料液比1∶2(g∶mL),pH 7.0,酶解温度50 ℃,酶解时间6 h。在此条件下,蓝圆鲹多肽(round scad peptides,RSPs)的XOD抑制活性为64.03%(15 g/L),Fe2+结合活性为61.56%;此法获得的RSPs分子质量92.35%分布在1 000 Da以下;氨基酸分析表明,RSPs中与XOD抑制活性有关的疏水性氨基酸(如Ala、Val、Leu、Ile)以及与金属离子结合活性相关的氨基酸(如Glu、Asp、Arg、His)含量较高;此外,紫外光谱和红外光谱扫描结果表明,RSPs可与Fe2+发生结合反应,其与Fe2+的结合位点可能是酰胺键、氨基的N原子与羧基的O原子。

关键词： 蓝圆鲹; 酶解; 制备; 黄嘌呤氧化酶抑制肽

本文引用格式

周雅 , 胡晓 , 李来好 , 杨贤庆 , 陈胜军 , 吴燕燕 , 杨少玲 . 蓝圆鲹黄嘌呤氧化酶抑制肽的制备及其活性分析[J]. 食品与发酵工业, 2021 , 47(13) : 146 -153 . DOI: 10.13995/j.cnki.11-1802/ts.026193

Abstract

Round scad (Decapterus maruadsi) muscle was hydrolyzed with five different proteases (trypsin, papain, neutrase, protomax and alcalase). The optimum conditions of enzymatic hydrolysis were obtained by single factor analysis and orthogonal test by the index of xanthine oxidase (XOD) inhibition activity. The optimum conditions of enzymatic hydrolysis were as follows: neutrase of 0.3% (mass fraction), the solid-liquid ratio of 1∶2 (g∶mL), pH 7.0, 50 ℃, and 6 h. Under the optimal conditions, the XOD inhibition activity of round scad peptides (RSPs) was 64.03% (15 g/L) and the Fe2+ chelating activity was 61.56%. The molecular weight of RSPs was 92.35% distributed below 1 000 Da. The analysis of amino acids showed that the content of hydrophobic amino acids related to XOD inhibition activity (such as Ala, Val, Leu, Ile) and metal ion binding activity (such as Glu, Asp, Arg, His) accounted for more. In addition, ultraviolet spectrum and Fourier transform infrared spectroscopy demonstrated that RSPs could bound with Fe2+, and the possible binding sites were located at amide bonds, nitrogen atom of the amino group or oxygen atom of the carboxyl group.

Key words： round scad (Decapterus maruadsi); enzymatic hydrolysis; preparation; xanthine oxidase inhibitory peptides

参考文献

[1] 王爽. 蓝圆鲹脱脂蛋白抗氧化肽的制备及其分离纯化、抗氧化活性研究[D].广州:华南农业大学,2018.
WANG S.Preparation,purification and antioxidant activity of Decapterus maruadsi peptide antioxidant peptide[D].Guangzhou:South China Agricultural University,2018.
[2] GONG F,CHEN M F,CHEN J L,et al.Boiled abalone byproduct peptide exhibits anti-tumor activity in HT1080 cells and HUVECs by suppressing the metastasis and angiogenesis in vitro[J].Journal of Agricultural and Food Chemistry,2019,67(32):8 855-8 867.
[3] 谢丽平. 具有抗氧化、抗衰老活性的多肽筛选、分离纯化及结构鉴定[D].广州:华南理工大学,2019.
XIE L P,Screening,separation and purification,structure identification of peptides with antioxidant and anti-aging effects[D].Guangzhou:South China University of Technology,2019.
[4] 李瑞杰, 胡晓,李来好,等.罗非鱼皮酶解物钙离子结合能力及其结合物的抗氧化活性[J].南方水产科学,2019,15(6):106-111.
LI R J,HU X,LI L H,et al.Calcium ion binding ability of tilapia skin hydrolysate and its antioxidant activity[J].South China Fisheries Science,2019,15(6):106-111.
[5] 朱晓连, 陈华,蔡冰娜,等.具有结合胆酸盐作用卵形鲳鲹蛋白酶解物的制备和分子量分布研究[J].南方水产科学,2017,13(2):101-108.
ZHU X L,CHEN H,CAI B N,et al.Production of bile acid salts binding hydrolysate from Trachinotus ovatus and its molecular weight distribution[J].South China Fisheries Science,2017,13(2):101-108.
[6] 李雯晖, 张健,应欣,等.大豆低聚肽对自发性高血压大鼠血压及血浆血管紧张素的影响[J].食品科学,2019,40(11):152-158.
LI W H,ZHANG J,YING X,et al.Effect of soybean oligopeptides on blood pressure and plasma angiotensin in spontaneously hypertensive rats[J].Food Science,2019,40(11):152-158.
[7] 延海莹, 刘盟梦,乔乐克,等.扇贝裙边活性肽的制备及其降血糖活性研究[J].食品工业,2018,39(3):117-121.
YAN H Y,LIU M M,QIAO L K,et al.Study on scallop skirt active peptide and glucose tolerance activity[J].The Food Industry,2018,39(3):117-121.
[8] 黎青勇. 核桃源降尿酸肽靶向抑制黄嘌呤氧化酶活性的构效机制研究[D].广州:华南理工大学,2018.
LI Q Y.Study on the structure-activity mechanism of targeting inhibition of xanthine oxidase by uric acid-lowering peptides derived from walnut[D].Guangzhou:South China University of Technology,2018.
[9] LIU N X,WANG Y,YANG M F,et al.New rice-derived short peptide potently alleviated hyperuricemia induced by potassium oxonate in rats[J].Journal of Agricultural and Food Chemistry.2019,67(1):220-228.
[10] 盛周煌. 罗非鱼皮胶原蛋白降尿酸活性肽的研究[D].广州:华南理工大学,2018.
SHENG Z H.Study on uric acid-reducing peptide of tilapia skin collagen[D].Guangzhou:South China University of Technology,2018.
[11] 邹琳. 鲣鱼黄嘌呤氧化酶抑制肽的酶解制备及功能活性评价[D].杭州:浙江大学,2019.
ZOU L.Enzymatic preparation and functional evaluation of xanthine oxidase inhibitory peptides from skipjack tuna[D].Hangzhou:Zhejiang University,2019.
[12] 李宇娟. 鲣鱼降尿酸肽的制备分离、结构表征及功效机制研究[D].广州:华南理工大学,2019.
LI Y J.Preparation,isolation,structural characterization,and the effect mechanism of uric acid-lowering peptides derived from bonito[D].Guangzhou:South China University of Technology,2019.
[13] MUROTA I,TAGUCHI S,SATO N,et al.Identification of antihyperuricemic peptides in the proteolytic digest of shark cartilage water extract using in vivo activity-guided fractionation[J].Journal of Agricultural & Food Chemistry,2014,62(11):2 392-2 397.
[14] HE W W,SU G W,SUN D X,et al.In vivo anti-hyperuricemic and xanthine oxidase inhibitory properties of tuna protein hydrolysates and its isolated fractions[J].Food Chemistry,2019,272:453-461.
[15] 王成华, 邢新会.黄嘌呤氧化酶的研究进展及其发展前景[J].广西科学,2017,24(1):15-24.
WANG C H,XING X H.Research progress and prospects of xanthine oxidase[J].Guangxi Sciences,2017,24(1):15-24.
[16] WU X H,RUAN J L,ZHANG J,et al.Pallidifloside D,a saponin glycoside constituent from Smilax riparia,resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice[J].Journal of Ethnopharmacology,2014,157(157):201-205.
[17] 杨伊然, 胡晓,杨贤庆,等.蓝圆鲹蛋白酶解物的螯合矿物离子活性研究[J].食品科学,2017,38(3):88-93.
YANG Y R,HU X,YANG X Q,et al.Mineral ion chelating activity of enzymatic protein hydrolysates from Decapterus maruadsi muscle[J].Food Science,2017,38(3):88-93.
[18] SUN N,CUI P B,JIN Z Q,et al.Contributions of molecular size,charge distribution,and specific amino acids to the iron-binding capacity of sea cucumber (Stichopus japonicus) ovum hydrolysates[J].Food Chemistry,2017,230:627-636.
[19] 赵谋明, 徐巨才,刘洋,等.秋刀鱼制备黄嘌呤氧化酶抑制肽的工艺优化[J].农业工程学报,2015,31(14):291-297.
ZHAO M M,XU J C,LIU Y,et al.Technology optimization on preparation of XOD inhibition peptide from saury[J].Transactions of the Chinese Society of Agricultural Engineering,2015,31(14):291-297.
[20] 田旭静, 段鹏慧,范三红,等.响应面法优化酶解藜麦糠蛋白制备抗氧化肽工艺[J].食品科学,2018,39(10):158-164.
TIAN X J,DUAN P H,FAN S H,et al.Response surface methodology for optimization of hydrolysis conditions for preparing antioxidant peptides from quinoa bran protein[J].Food Science,2018,39(10):158-164.
[21] 王婷婷. 蓝圆鲹蛋白酶解物的制备及其抗氧化活性研究[D].上海:上海海洋大学,2015.
WANG T T.Study on preparation of pound scad (Decapterus maruadsi) protein hydrolysates and its antioxidant activity[D].Shanghai:Shanghai Ocean University,2015.
[22] 何伟炜. 金枪鱼黄嘌呤氧化酶抑制肽的分离鉴定及其作用机制初探[D].广州:华南理工大学,2019.
HE W W.Study on identification and mechanism of xanthine oxidase inhibitory peptides from tuna protein[D].Guangzhou:South China University of Technology,2019.
[23] LI Q Y,KANG X Y,SHI C C,et al.Moderation of hyperuricemia in rats via consuming walnut protein hydrolysates diet and identification of new antihyperuricemic peptides[J].Food and Function,2018,9(1):107-116.
[24] VATTEM D A,MAHONEY R R.Production of dialysable iron by in vitro digestion of chicken muscle protein fractions:The size of the dialysable iron[J].Journal of the Science of Food and Agriculture,2005,85(9):1 537-1 542.
[25] TORRES-FUENTES C,ALAIZ M,VIOQUE J.Iron-chelating activity of chickpea protein hydrolysate peptides[J].Food Chemistry,2012,134(3):1 585-1 588.
[26] CARRASCO-CASTILLA J,HERNÁNDEZ-ÁLVAREZ A J,JIMÉN-EZ-MARTÍNEZ C,et al.Antioxidant and metal chelating activities of Phaseolus vulgaris L.var.Jamapa protein isolates,phaseolin and lectin hydrolysates[J].Food Chemistry,2012,131(4):1 157-1 164.
[27] ZACHARIOU M,HEARN M T W.Application of immobilized metal ion chelate complexes as pseudocation exchange adsorbents for protein separation.[J].Biochemistry,1996,35(1):202-211.
[28] CAETANO-SILVA M E,BERTOLDO-PACHECO M T,PAES-LEME A F,et al.Iron-binding peptides from whey protein hydrolysates:Evaluation,isolation and sequencing by LC-MS/MS[J].Food Research International,2015,71:132-139.
[29] 纪晓雯, 王志耕,阚文翰,等.酪蛋白铁螯合肽的分离纯化及结构解析[J].食品科学,2018,39(6):63-68.
JI X W,WANG Z G,KAN W H,et al.Isolation,purification and structural analysis of iron-binding peptides from casein hydrolysate[J].Food Science,2018,39(6):63-68.
[30] MIZUGUCHI M,FUJISAWA R,NARA M,et al.Fourier-transform infrared spectroscopic study of Ca2+-binding to osteocalcin[J].Calcified Tissue International,2001,69(6):337-342.
[31] YUAN B,ZHAO C,CHENG C,et al.A peptide-Fe(II) complex from Grifola frondosa protein hydrolysates and its immunomodulatory activity[J].Food Bioscience,2019,32:100 459.
[32] WU W F,LI B F,HOU H,et al.Identification of iron-chelating peptides from pacific cod skin gelatin and the possible binding mode[J].Journal of Functional Foods,2017,35:418-427.
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